ZitB (YbgR), a Member of the Cation Diffusion Facilitator Family, Is an Additional Zinc Transporter in <i>Escherichia coli</i>

Journal of Bacteriology - Tập 183 Số 15 - Trang 4664-4667 - 2001
Gregor Grass1, Baochao Fan2, Barry P. Rosen2, Sylvia Franke3,4,5, Dietrich H. Nies3,4,5, Christopher Rensing1
1Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 857211;
2Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 482012; and
3Department of Soil, Wa-ter, and Environmental Science, University of Arizona, Shantz Bldg. #38 Rm. 429, Tucson, AZ 85721.
4Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona, 85721
5Institut für Mikrobiologie, Martin-Luther-Universität Halle-Wittenberg, 06120 Halle, Germany3

Tóm tắt

ABSTRACT The Escherichia coli zitB gene encodes a Zn(II) transporter belonging to the cation diffusion facilitator family. ZitB is specifically induced by zinc. ZitB expression on a plasmid rendered zntA -disrupted E. coli cells more resistant to zinc, and the cells exhibited reduced accumulation of 65 Zn, suggesting ZitB-mediated efflux of zinc.

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Tài liệu tham khảo

10.1128/JB.181.22.6876-6881.1999

Beard S. J. Hashim R. Membrillo-Hernandez J. Hughes M. N. Poole R. K. Zinc(II) tolerance in Escherichia coli K12; evidence that the zntA gene (o732) encodes a cation transport ATPase.Mol. Microbiol.251997883891

Beard S. J. Hashim R. Wu G. Binet M. R. B. Hughes M. N. Poole R. K. Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli.FEMS Microbiol. Lett.1842000231235

10.1046/j.1365-2958.1999.01229.x

Datsenko K. A. Wanner B. L. One-step inactivation of chromosomal genes in Escherichia coli K12 using PCR products.Proc. Natl. Acad. Sci. USA97200066406645

Eide D. J. The molecular biology of metal ion transport in Saccharomyces cerevisiae.Annu. Rev. Nutr.181998441469

Franke S. Grass G. Nies D. H. The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions.Microbiology1472000965972

Grass G. Große C. Nies D. H. Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.J. Bacteriol.182200113901398

10.1128/JB.181.8.2385-2393.1999

Huang L. Gitschier J. A novel gene involved in zinc transport is deficient in the lethal milk mouse.Nat. Genet.171997292297

Kuroda M. Hayashi H. Ohta T. Chromosome-determined zinc-responsible operon czr in Staphylococcus aureus strain 912.Microbiol. Immunol.431999115125

10.1128/jb.179.20.6228-6237.1997

10.1128/jb.162.1.328-334.1985

Miller J. H. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. 1992 Cold Spring Laboratory Press Cold Spring Harbor N.Y

Miyabe S. Izawa S. Inoue Y. Expression of ZRC1 coding for suppressor of zinc toxicity is induced by zinc-starvation stress in Zap1-dependent fashion in Saccharomyces cerevisiae.Biochem. Biophys. Res. Comm.2762000879884

Mobley H. L. T. Rosen B. P. Energetics of plasmid-mediated arsenate resistance in Escherichia coli.Proc. Natl. Acad. Sci. USA79198261196122

Nies D. H. Silver S. Ion efflux systems involved in bacterial metal resistances.J. Ind. Microbiol.141995186199

Outten C. E. Outten F. W. O'Halloran T. V. DNA distortion mechanism for transcriptional activation by ZntR, a Zn(II)-responsive MerR homologue in Escherichia coli.J. Biol. Chem.27419993751737524

10.1002/j.1460-2075.1995.tb07042.x

Palmiter R. D. Cole T. B. Findley S. D. ZnT-2, a mammalian protein that confers resistance to zinc by facilitating vesicular sequestration.EMBO J.15199617841791

Palmiter R. D. Cole T. B. Quaife C. J. Findley S. D. ZnT-3, a putative transporter of zinc into synaptic vesicles.Proc. Natl. Acad. Sci. USA9319961493414939

10.1046/j.1365-2958.1998.00883.x

Paulsen I. T. Saier M. J. A novel family of ubiquitous heavy metal ion transport proteins.J. Membr. Biol.156199799103

Rensing C. Mitra B. Rosen B. P. The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase.Proc. Natl. Acad. Sci. USA9419971432614331

Rensing C. Sun Y. Mitra B. Rosen B. P. Pb(II)-translocating P-type ATPases.J. Biol. Chem.27319983261432617

10.1128/JB.180.16.4024-4029.1998