Tác động độc hại của hạt nano Zirconia trong các tế bào giống như nguyên bào xương 3T3-E1

Nanoscale Research Letters - Tập 13 - Trang 1-12 - 2018
Mingfu Ye1,2, Bin Shi1,3
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedical Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China
2Department of Implantology, Xiamen Stomatology Hospital, Hospital and School of Stomatology, Xiamen Medical University, Xiamen, People’s Republic of China
3Department of Implantology, School and Hospital of Stomatology, Wuhan University, Wuhan, People’s Republic of China

Tóm tắt

Zirconia (ZrO2) là một trong những oxit kim loại được sử dụng rộng rãi cho các ứng dụng sinh học tiềm năng như cảm biến sinh học, liệu pháp ung thư, cấy ghép, và nha khoa nhờ vào độ bền cơ học cao và độ độc thấp. Do sự ứng dụng rộng rãi này, khả năng tiếp xúc với các hạt nano (NPs) đã tăng lên, điều này đã thu hút sự chú ý đáng kể. Do đó, việc nghiên cứu hồ sơ độc tính của hạt nano ZrO2 là rất cấp bách. Titanium dioxide (TiO2) là một vật liệu nano khác được sử dụng rộng rãi, được biết đến với độc tính yếu. Trong nghiên cứu này, các hạt nano TiO2 được sử dụng làm kiểm soát để đánh giá tính tương hợp sinh học của hạt nano ZrO2. Chúng tôi phát hiện độc tính tế bào của các hạt nano TiO2 và ZrO2 trên các tế bào 3T3-E1 như nguyên bào xương và nhận thấy các loài oxy phản ứng (ROS) đóng vai trò quan trọng trong độc tính do hạt nano TiO2 và ZrO2 gây ra theo phương thức phụ thuộc vào nồng độ. Chúng tôi cũng cho thấy rằng các hạt nano TiO2 và ZrO2 có thể gây ra hiện tượng apoptosis và thay đổi hình thái sau khi nuôi cấy với tế bào 3T3-E1 ở nồng độ cao. Hơn nữa, các hạt nano TiO2 và ZrO2 ở nồng độ cao có thể ức chế sự khác biệt tạo xương của tế bào, so với các hạt nano ở nồng độ thấp. Kết luận, các hạt nano TiO2 và ZrO2 có thể gây ra phản ứng độc tế bào in vitro theo phương thức phụ thuộc vào nồng độ, điều này cũng có thể ảnh hưởng đến quá trình tạo xương; các hạt nano ZrO2 cho thấy tác động độc hại mạnh hơn so với hạt nano TiO2.

Từ khóa

#Zirconia #hạt nano #độc tính #tế bào nguyên bào xương #oxy phản ứng #apoptosis

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