Zinc and ascorbic acid treatment alleviates systemic inflammation and gastrointestinal and renal oxidative stress induced by sodium azide in rats
Tóm tắt
Sodium azide (NaN3) is a chemical of rapidly increasing economic importance but with high toxic attributes. In this study, the effects of zinc (Zn) and ascorbic acid (AsA) supplementation on sodium azide (NaN3)-induced toxicity in the stomach, colon and kidneys were evaluated in Wistar rats. Twenty-eight rats were randomly allocated to four experimental groups as follows: group A (control) given distilled water only; group B (NaN3 only, 20 mg/kg); group C (NaN3 + zinc sulphate, ZnSO4 80 mg/kg); and group D (NaN3 + AsA 200 mg/kg). NaN3 was found to significantly (p < 0.05) induce increases in serum nitric oxide (NO), advanced oxidation protein products (AOPP), myeloperoxidase (MPO) and total protein levels, along with significant (p < 0.05) increase in gastric, colonic and renal malondialdehyde (MDA) and protein carbonyl (PCO) levels. In addition, NaN3 induced significant (p < 0.05) reduction in superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities in the colon and kidneys. Treatment with Zn or AsA caused significant (p < 0.05) reduction in serum levels of oxidative and inflammatory markers, as well as tissue PCO and MDA levels. Moreover, co-treatment with Zn or AsA significantly (p < 0.05) restored colonic and renal levels of antioxidant enzymes, reduced glutathione and protein thiols. This study shows that Zn or AsA supplementation alleviated NaN3 toxicity by suppressing systemic inflammation and preventing oxidative damage in the stomach, colon and kidneys of rats.
Tài liệu tham khảo
Chang S, Lamm SH (2003) Human health effects of sodium azide exposure: a literature review and analysis. Int J Toxicol 22(3):175–186. https://doi.org/10.1080/10915810305109
Betterton EA (2003) Environmental fate of sodium azide derived from automobile airbags. Crit Rev Environ Sci Tech 33(4):423–458. https://doi.org/10.1080/10643380390245002
Rippen HE, Lamm SH, Nicoll PG, Cummings L, Howearth G, Thayer D (1996) Occupational health data as a basis for process engineering changes: development of a safe work environment in the sodium azide industry. Int Arch Occup Environ Health 68(6):459–468. https://doi.org/10.1007/BF00377870
Qamirani E, Razavi HM, Wu X, Davis MJ, Kuo L, Hein TW (2006) Sodium azide dilates coronary arterioles via activation of inward rectifier K+ channels and Na+ -K+ -ATPase. Am J Physiol Heart Circ Physiol 290(4):H1617–H1623
Faqi AS, Richards D, Hauswirth JW, Schroeder R (2008) Maternal and developmental toxicity study of sodium azide in rats. Reg Toxicol Pharmacol 52(2):158–162. https://doi.org/10.1016/j.yrtph.2008.08.001
Van Laar VS, Roy N, Liu A, Rajprohat S, Arnold B, Dukes AA et al (2014) Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy. Neurobiol Dis 74C:180–193. https://doi.org/10.1016/j.nbd.2014.11.015
Gao C, Chang P, Yang L et al (2018) Neuroprotective effects of hydrogen sulfide on sodium azide-induced oxidative stress in PC12 cells. Int J Mol Med 41(1):242–250. https://doi.org/10.3892/ijmm.2017.3227
Bennett MC, Mlady GW, Fleshner M, Rose GM (1996) Synergy between chronic corticosterone and sodium azide treatments in producing a spatial learning deficit and inhibiting cytochrome oxidase activity. Proc Natl Acad Sci USA 93(3):1330–1334. https://doi.org/10.1073/pnas.93.3.1330
Dukoff DJ, Hogg DW, Hawrysh PJ, Buck LT (2014) Scavenging ROS dramatically increase NMDA receptor whole-cell currents in painted turtle cortical neurons. J Exp Biol 217(Pt 18):3346–3355. https://doi.org/10.1242/jeb.105825
Liu Z, Ren Z, Zhang J, Chuang CC, Kandaswamy E, Zhou T et al (2018) Role of ROS and nutritional antioxidants in human diseases. Front Physiol 9:477. https://doi.org/10.3389/fphys.2018.00477
Smith OB, Akinbamizo OO (2000) Micronutrients and reproduction in farm animals. Anim Reprod Sci 60–61:549–560. https://doi.org/10.1016/s0378-4320(00)00114-7
Colagar AH, Marzony ET, Chaichi MJ (2009) Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr Res 29(2):82–88. https://doi.org/10.1016/j.nutres.2008.11.007
Lee SR (2018) Metal and metalloid-induced oxidative damage: biological importance of potential antioxidants. Oxid Med Cell Longev. https://doi.org/10.1155/2018/9156285
Maret W (2008) Metallothionein redox biology in the cyto-protective and cytotoxic functions of Zinc. Exp Gerontol 43(5):363–369. https://doi.org/10.1016/j.exger.2007.11.005
Skrovanek S, DiGuilio K, Bailey R, Huntington W, Urbas R, Mayilvaganan B et al (2014) Zinc and gastrointestinal disease. World J Gastrointest Pathophysiol 5(4):496–513. https://doi.org/10.4291/wjgp.v5.i4.496
Kalender S, Uzun FG, Durak D, Demir F, Kalender F (2010) Malathion-induced hepatotoxicity in rats: the effects of vitamins C and E. Food Chem Toxicol 48(2):633–638. https://doi.org/10.1016/j.fct.2009.11.044
Shyu K-G, Chang C-C, Yeh Y-C, Sheu J-R, Chou DS (2014) Mechanisms of ascorbyl radical formation in human platelet-rich plasma. Biomed Res Int. https://doi.org/10.1155/2014/614506
Padayatty S, Levine M (2016) Vitamin C; the known and the unknown and Goldilocks. Oral Dis 22(6):463–493. https://doi.org/10.1111/odi.12446
Aditi A, Graham DY (2012) Vitamin C, gastritis, and gastric disease: a historical review and update. Dig Dis Sci 57(10):2504–2515. https://doi.org/10.1007/s10620-012-2203-7
Olajide OJ, Enaibe BU, Bankole OO, Akinola OB, Laoye BJ, Ogundele OM (2016) Kolaviron was protective against sodium azide (NaN3) induced oxidative stress in the pre-frontal cortex. Metab Brain Dis 31(1):25–35. https://doi.org/10.1007/s11011-015-9674-0
Troskot B, Simicevic VN, Dodig M, Rotivic I, Ivankovic D, Dunvnjak M (1997) The protective effect of Zinc sulphate pre-treatment against duodenal ulcers in the rat. Biometals 10(4):325–329. https://doi.org/10.1023/A:1018332618512
Fetoui H, Makni M, Garoui M, Zeghal N (2010) Toxic effects of lambda-cyhalothrin, a synthetic pyrethroid pesticide, on the rat kidney: involvement of oxidative stress and protective role of ascorbic acid. Exp Toxicol Pathol 62(6):593–599. https://doi.org/10.1016/j.etp.2009.08.004
Djeffal A, Messarah M, Boumendjel A, Kadeche L, Feki AE (2012) Protective effects of Vitamin C and selenium supplementation of methomyl-induced tissue oxidative stress in adult rats. Toxicol Ind Health 31(1):31–43. https://doi.org/10.1177/07482-33712468020
PHS (PUBLIC HEALTH SERVICE) (1996) Public health service policy on humane care and the use of laboratory animals. US Department of Health and Humane Services, Washington, DC, pp 99–158
Olaleye SB, Adaramoye OA, Erigbali PP, Adeniyi OS (2007) Lead exposure increases oxidative stress in the gastric mucosa of HCl/-ethanol-exposed rats. World J Gastroenterol 13(38):5121–5126. https://doi.org/10.3748/wjg.v13.i38.5121
Kayali R, Cakatay U, Akcay T, Altug T (2006) Effect of alpha-lipoic acid supplementation on markers of protein oxidation in post-mitotic tissues of ageing rat. Cell Biochem Funct 24(1):79–85. https://doi.org/10.1002/cbf.1190
Xia Y, Tsai AL, Berka V et al (1998) Superoxide generation from endothelial nitric oxide synthase. A C2+/calmodulin-dependent and tetrahydrobiopterin regulatory process. J Biol Chem 273(40):25804–25808. https://doi.org/10.1074/jbc.273.40.25804
Wolff SF (1994) Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydrogen peroxides. Methods Enzymol 233(2):182–189. https://doi.org/10.1016/0003-2697(92)90122-n
Varshney R, Kale RK (1990) Effect of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol 58(5):733–743. https://doi.org/10.1080/09553009014552121
Reznick AZ, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357–363. https://doi.org/10.1016/s0076-6879(94)33041-7
Jollow DJ, Mitchell JR, Zampaglione N (1974) Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 11(3):151–169. https://doi.org/10.1159/000136485
Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82(1):70–77. https://doi.org/10.1016/0003-9861(59)90090-6
Rotruck JT (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179(4073):588–590. https://doi.org/10.1126/science.179.4073.588
Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 25:7130–7139 PMID: 4436300
Misra HP, Fridovich I (1972) The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247(10):3170–3175. https://doi.org/10.1016/S0021-9258(19)45228-9 PMID: 4623845
Gornal AG, Bardawill JC, David MM (1949) Determination of serum proteins by means of biuret reaction. J Biol Chem 177(2):751–766. https://doi.org/10.1016/S0021-9258(18)57021-6 PMID:18110453
Aycan IO, Elpek O, Akkay B, Kıraç E, Tuzcuc H, Kayac S et al (2018) Diclofenac induced gastrointestinal and renal toxicity is alleviated by thymoquinone treatment. Food Chem Toxicol 118:795–804. https://doi.org/10.1016/j.fct.2018.06.038
Liu B, Che W, Zheng C, Liu W, Wen J, Fu H et al (2013) SIR T5: a safeguard against oxidative stress-induced apoptosis in cardiomyocytes. Cell Physiol Biochem 32(4):1050–1059. https://doi.org/10.1159/000354505
Demir E, Kaya B, Soriano C, Creus A, Marcos R (2011) Genotoxic analysis of four lipid-peroxidation products in the mouse lymphoma assay. Mut Res 726(2):98–103. https://doi.org/10.1016/j.mrgentox.2011.07.001
Chevion M, Berenshtein E, Stadtman ER (2000) Human studies related to protein oxidation: protein carbonyl content as a marker of damage. Free Radic Res 33(Suppl):S99–S108
Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milazani A (2006) Biomarkers of oxidative stress in human disease. Clin Chem 52(4):601–623. https://doi.org/10.1373/clinchem.2005.061408
Adedara IA, Teberen R, Ebokaiwe AP, Ehwerhemuepha T, Farombi EO (2012) Induction of oxidative stress in liver and kidney of rats exposed to Nigerian bonny light crude oil. Environ Toxicol 27(6):372–379. https://doi.org/10.1002/tox.20660
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V et al (2017) Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev. https://doi.org/10.1155/2017/8416763
Davies MJ (2011) Myeloperoxidase-derived oxidation: mechanisms of biological damage and its prevention. J Clin Biochem Nutr 48(1):8–19. https://doi.org/10.3164/jcbn.11-006FR
Piwowar A (2010) Advanced oxidation protein products. Part I. Mechanism of the formation, characteristics and property. Polski Merkuriusz Lekarski 28(164):166–169
Selmeci L (2011) Advanced oxidation protein products (AOPP): novel uremic toxins, or components of the non-enzymatic antioxidant system of the plasma proteome? Free Radic Res 45(10):1115–1123. https://doi.org/10.3109/10715762.2011.602074
Bryan NS, Grisham MB (2007) Methods to detect nitric oxide and its metabolites in biological samples. Free Radic Biol Med 43(5):645–657. https://doi.org/10.1016/j.freeradbiomed.2007.04.026
Laskin JD, Heck DE, Laskin DL (1994) Multifunctional role of nitric oxide in inflammation. Trends Endocrinol Metab 5(9):377–382. https://doi.org/10.1016/1043-2760(94)90105-8
Rawi SM, Seif Al Nassr FM (2013) Zinc sulphate and vitamin E alleviate reproductive toxicity caused by aluminium sulphate in male albino rats. Toxicol Ind Health 31(3):221–234. https://doi.org/10.1177/0748233712469650
Prasad AS (2014) Zn is an antioxidant and anti-inflammatory agent: its role in human health. Front Nutr 1:14. https://doi.org/10.3389/fnut.2014.00014
Bao B, Ahmad A, Azmi A, Li Y, Prasad AS, Sarkar FH (2013) The biological significance of Zn in inflammation and aging. In: Rahman I, Bagchi D (eds) Inflammation, advancing and nutrition. Elsevier Inc, New York, pp 15–27
Jurczuk M, Brzóska MM, Moniuszko-Jakoniuk J (2007) Hepatic and renal concentrations of vitamins E and C in lead- and ethanol-exposed rats. An assessment of their involvement in the mechanisms of peroxidative damage. Food Chem Toxicol 45(8):1478–1486. https://doi.org/10.1016/j.fct.2007.02.007
Abbas M, Siddiqi MH, Khan K, Zahra K, Naqvi AU (2017) Haematological evaluation of sodium fluoride toxicity in Oryctolagus cunniculus. Toxicol Rep 19(4):450–454. https://doi.org/10.1016/j.toxrep.2017.07.002
Agbasi PU, Abasi N, Onye JJ, Ibeawuchi C, Uzoechi SC, Alagwu EA et al (2015) The effect of subchronic low dose of DDVP and sodium azide on the haematological indices of albino rats. World J Pharm Pharmaceut Sci 4(4):103–110
Naidu KA (2003) Vitamin C in human health and disease is still a mystery? An overview. Nutr J 2(1):7. https://doi.org/10.1186/1475-2891-2-7
Kelada SN, Shelton E, Kaufmann RB et al (2001) δ-Aminolevulinic acid dehydratase genotype and lead toxicity: a HuGE review. Am J Epidemiol 154(1):1–13. https://doi.org/10.1093/aje/154.1.1
Rahfiludin MZ, Ginandjar P (2013) The effect of Zn and vitamin C supplementation on hemoglobin and hematocrit levels and immune response in patients with Plasmodium vivax malaria. Southeast Asian J Trop Med Public Health 44(5):733–739
Somade OT, Olorode SK, Olaniyan TO, Faokunla O (2016) Quercetin, a polyphenolic phytochemical prevents sodium azide-induced extra-hepatic oxidative stress in rats. Cog Biol 2(1):1200798. https://doi.org/10.1080/23312025.2016.1200798