Zebrafish Larvae as a Behavioral Model in Neuropharmacology

Biomedicines - Tập 7 Số 1 - Trang 23
Ram Manohar Basnet1, Daniela Zizioli2, Somrat Taweedet3, Dario Finazzi4,5, Maurizio Memo6
1Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. [email protected].
2Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. [email protected].
3Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. [email protected].
4Clinical Chemistry Laboratory, ASST-Spedali Civili di Brescia, 25123 Brescia, Italy. [email protected].
5Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. [email protected].
6Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy. [email protected].

Tóm tắt

Zebrafish larvae show a clear and distinct pattern of swimming in response to light and dark conditions, following the development of a swim bladder at 4 days post fertilization. This swimming behavior is increasingly employed in the screening of neuroactive drugs. The recent emergence of high-throughput techniques for the automatic tracking of zebrafish larvae has further allowed an objective and efficient way of finding subtle behavioral changes that could go unnoticed during manual observations. This review highlights the use of zebrafish larvae as a high-throughput behavioral model for the screening of neuroactive compounds. We describe, in brief, the behavior repertoire of zebrafish larvae. Then, we focus on the utilization of light-dark locomotion test in identifying and screening of neuroactive compounds.

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