Yeast RNA Polymerase II Genes: Isolation with Antibody Probes

American Association for the Advancement of Science (AAAS) - Tập 222 Số 4625 - Trang 778-782 - 1983
Richard A. Young1, Ronald W. Davis1
1Department of Biochemistry, Stanford University School of Medicine, Stanford, California 94305

Tóm tắt

Genes encoding yeast RNA polymerase II subunits were cloned. Efficient isolation of these genes was accomplished by probing a phage λgt11 recombinant DNA expression library with polyvalent antibodies directed against purified yeast RNA polymerase II. The identity of genes that specify the largest RNA polymerase II subunits, the 220,000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay. Both of these genes exist in single copy in the yeast Saccharomyces cerevisiae .

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Tài liệu tham khảo

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American Association for the Advancement of Science (AAAS)

Tập 222 Số 4625

778-782

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