Widespread pyrethroid resistance in Australian diamondback moth,Plutella xylostella(L.), is related to multiple mutations in theparasodium channel gene

Bulletin of Entomological Research - Tập 101 Số 4 - Trang 393-405 - 2011
N. M. Endersby1, K. Viduka2, Simon W. Baxter3, Jarel T.S. Saw2, David G. Heckel4, Stephen W. McKechnie2
1School of Biological Sciences, Monash University, Victoria, Australia. [email protected]
2School of Biological Sciences, Monash University, Victoria, 3800, Australia
3University of Cambridge
4Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society

Tóm tắt

AbstractPopulations ofPlutella xylostella, extending over 3800 km in southern Australia, show no genetic structure as assessed by microsatellite markers; yet outbreaks of pyrethroid resistance occur sporadically in cropping areas. Since mutations in theparavoltage-gated sodium channel gene have been implicated in pyrethroid resistance, we looked for DNA sequence variation at this target among Australian moths. We found two resistance mutations previously reported for this species (L1014F and T929I), as well as a novel substitution (F1020S). Of the eight possible haplotypes formed by combinations of these three biallelic polymorphisms, only four were found in Australian populations: thewild-typeallele (w), thekdrmutation allele (kdr) with only L1014F, thesuper-kdr-likecombination of L1014F and T929I (skdrl), and thecrashdownallele with only F1020S (cdr). Comparison of genotype frequencies among survivors of permethrin assays with those from untreated controls identified three resistant genotypes:skdrlhomozygotes,cdrhomozygotes and the corresponding heterozygote,cdr/skrdl– the heterozygote being at least as resistant as either homozygote. Spatial heterogeneity of allele frequencies was conspicuous, both across the continent and among local collections, consistent with reported spatial heterogeneity of pyrethroid resistance. Further, high resistance samples were sometimes associated with high frequency ofcdr, sometimes high frequency ofskdrl,or sometimes with a high combinedcdr+skdrlfrequency. Theskdrlandcdralleles explain a high proportion of the Australia-wide resistance variation. These data add to evidence that nerve insensitivity by mutations in thepara-sodium channelgene is a common pyrethroid resistance mechanism inP. xylostella.

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Bulletin of Entomological Research

Tập 101 Số 4

393-405

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