White cell apoptosis in packed red cells

Transfusion - Tập 38 Số 11-12 - Trang 1082-1089 - 1998
Flavia Frabetti1, D. Musiani2, Marina Marini3, Claudia Fanelli4, Simona Coppola5, Lina Ghibelli6, Pier Luigi Tazzari7, Andrea Bontadini8, Cristina Tassi9, Roberto Conte
1Flavia Frabetti, PhD, Fellow, Istituto Superiore di Sanità, Istituto di Istologia ed Embriologia Generale, Università di Bologna.
2Daniela Musiani, MS, Fellow, Istituto di Istologia ed Embriologia Generale, Università di Bologna.
3Marina Marini, PhD, Professor of Cell Biology, Istituto di Istologia ed Embriologia Generale, Università di Bologna.
4Claudia Fanelli, MS, Fellow, Istituto Superiore di Sanità, Dipartimento di Biologia, Università di Tor Vergata, Rome, Italy.
5Simona Coppola, MS, Fellow, Dipartimento di Biologia, Università di Tor Vergata.
6Lina Ghibelli, PhD, Researcher, Dipartimento di Biologia, Università di Tor Vergata.
7Pier Luigi Tazzari, MD, Clinical Assistant, Servizio di Immunoematologia e Trasfusionale, Policlinico S. Orsola, Bologna.
8Andrea Bontadini, MD, Clinical Assistant, Servizio di Immunoematologia e Trasfusionale, Policlinico S. Orsola.
9Cristina Tassi, MD, Clinical Assistant, Servizio di Immunoematologia e Trasfusionale, Policlinico S. Orsola.

Tóm tắt

BACKGROUND: After the removal of the buffy coat, packed red cell (RBC) transfusion units still contain white cells that may undergo apoptosis as a result of storage conditions (1‐6 degrees C). The aim of the present study was the evaluation of this phenomenon in view of the possible influence it may have on febrile nonhemolytic transfusion reactions. STUDY DESIGN AND METHODS: Three independent methods (microscopy, DNA electrophoresis, and cytometry) were used to evaluate apoptosis in white cells present in 13 RBC units. Of these units, 10 had been collected into CPD/saline‐adenine‐glucose‐mannitol and 3 into CPDA‐1; each bag was split in two parts, one of which was irradiated. RBCs were stored at 1 to 6 degrees C, and samples were periodically withdrawn for study. The proliferative capacity of stored lymphocytes was evaluated after phytohemagglutinin stimulation and tritiated thymidine incorporation. RESULTS: Apoptosis was found to occur in both granulocytes and lymphocytes, starting from the first 48 to 72 hours of storage. The choice of the anticoagulant‐preservative solution and the effect of irradiation did not influence the amount and the timing of the apoptotic phenomenon. Lymphocyte proliferative capacity was found to decrease sharply with storage time. CONCLUSION: Conditions of storage in RBCs induce consistent apoptosis in residual white cells. The possible clinical implications of the relationships between apoptosis and the induction of biologic response modifiers (that may cause interleukin‐mediated febrile non‐hemolytic transfusion reactions) and between apoptosis and immune reactions remain to be elucidated.

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Tài liệu tham khảo

10.1002/path.1711050103

10.1016/0167-5699(93)90214-6

10.1016/S1056-8719(97)00033-6

10.1016/0011-2240(90)90021-U

10.1016/0014-5793(90)80040-P

10.1002/jcp.1041530115

10.1002/ijc.2910570321

10.1002/jlb.57.1.2

10.1161/01.ATV.16.1.19

10.1182/blood.V89.6.1931

10.1016/S1054-3589(08)61060-1

10.1097/00062752-199502060-00013

10.1046/j.1537-2995.1994.34994378276.x

Heddle NM, 1996, Transfusion reactions, 45

10.1046/j.1537-2995.1997.37797369441.x

Reynolds CP, 1986, Sensitive method for detecting viable cells seeded into bone marrow, Cancer Res, 46, 5878

10.1016/S0074-7696(08)62312-8

10.1006/excr.1993.1176

10.1016/0022-1759(91)90198-O

10.1016/0022-1759(94)90373-5

10.1002/(SICI)1097-0215(19961104)68:3<349::AID-IJC13>3.0.CO;2-3

10.1080/095530096145076

10.1126/science.7533326

10.1084/jem.184.2.429

10.1046/j.1537-2995.1993.33393174443.x

10.1016/S0268-960X(95)90028-4

Stack G., 1997, Cytokines in transfusion medicine: a primer, 21

10.1159/000237385

10.1080/09553009014551621

Pelszynski MM, 1994, Effect of γirradiation of red blood cell units on T‐cell inactivation as assessed by limiting dilution analysis: implications for preventing transfusion‐associated graft‐versus‐host disease, Blood, 83, 1683, 10.1182/blood.V83.6.1683.1683

10.1016/S0140-6736(69)90867-8

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Transfusion

Tập 38 Số 11-12

1082-1089

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