Wave of cortical actin polymerization in the sea urchin egg

Wiley - Tập 7 Số 1 - Trang 46-53 - 1987
Shigenobu Yonemura1, Issei Mabuchi2,3
1Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro‐ku, Tokyo, Japan
2Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan
3Department of Cell Biology, National Institute for Basic Biology, Okazaki, Aichi, Japan

Tóm tắt

AbstractThe distribution of actin filaments in the cortical layer of sea urchin eggs during fertilization has been investigated by light microscopy using fluorescently labeled phallotoxins. The cortical layer of both whole eggs and cortices isolated on a glass surface was examined. In cortices of unfertilized eggs, numerous fluorescent spots were seen, which may correspond to short actin filament cores in microvilli. After insemination, one of the sperm‐attaching points on the egg surface first became strongly fluorescent. This fluorescence grew around the point of sperm penetration with the growth of the fertilization cone. Then, the cortical layer of the egg around the fertilization cone became strongly fluorescent and the fluorescence propagated in a wavelike manner over the entire cortex. The mechanism of the propagation of actin polymerization is discussed.

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Tài liệu tham khảo

10.1073/pnas.77.2.980

10.1083/jcb.83.1.241

10.1083/jcb.93.1.24

10.1016/0012-1606(82)90301-3

10.1016/0012-1606(81)90463-2

10.1002/cm.970030518

10.1016/0012-1606(83)90254-3

10.1083/jcb.71.1.35

10.1083/jcb.99.5.1647

10.1016/0014-4827(70)90257-0

10.1016/0014-4827(83)90443-3

10.1083/jcb.76.2.448

10.1111/j.1440-169X.1982.00443.x

10.1002/cm.970020203

10.1083/jcb.87.3.841

10.1083/jcb.99.3.994

10.1093/oxfordjournals.jbchem.a134115

10.1111/j.1432-1033.1986.tb09387.x

10.1038/262661a0

10.1083/jcb.66.2.305

10.1016/0012-1606(80)90443-1

Mabuchi I., 1981, Purification from starfish eggs of a protein that depolymerizes actin, J. Biochem., 89, 1341

10.1093/oxfordjournals.jbchem.a134068

10.1083/jcb.97.5.1612

10.1083/jcb.100.2.375

10.2220/biomedres.3.465

10.2220/biomedres.1.417

Mabuchi I., 1981, Formation of actin paracrystals from sea urchin egg extract under actin polymerizing conditions, Biomed. Res., 2, 143, 10.2220/biomedres.2.143

10.1016/0092-8674(79)90154-5

10.1016/0012-1606(84)90275-6

10.1016/0014-4827(76)90444-4

10.1016/0012-1606(79)90030-7

10.2307/1541271

10.1038/272253a0

10.1016/0045-6039(82)90036-7

10.1083/jcb.82.1.212

10.1016/0012-1606(77)90084-7

Tilney L. G., 1975, Molecules and Cell Movement, 339

10.1083/jcb.87.3.771

10.1016/0012-1606(81)90366-3

10.1016/0012-1606(80)90465-0

10.1083/jcb.99.3.844

10.1083/jcb.81.3.672

10.1016/0012-1606(86)90238-1

Yonemura S., 1985, Changes in the cortical actin filament distribution of the sea urchin egg during fertilization, Zool. Sci., 2, 949

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Wiley

Tập 7 Số 1

46-53

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