Vitexin, orientin and other flavonoids from Spirodela polyrhiza inhibit adipogenesis in 3T3‐L1 cells

Phytotherapy Research - Tập 24 Số 10 - Trang 1543-1548 - 2010
Jin-Pyo Kim1, Ik‐Soo Lee1, JeongJu Seo2, Mun Yhung Jung3, Young‐Hee Kim4, Nam–Hui Yim1, KiHwan Bae1
1College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea
2Department of Molecular Medicine, Kyungpook National University School of Medicine, Korea
3Department of Food Industry and Management, Woosuk University, Samrea-Up, Wanju-Kun, Jeonbuk 565-701, Korea
4Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, #N#Korea

Tóm tắt

AbstractTo investigate the adipogenesis inhibitory effect on lipid accumulation, 3T3‐L1 cells were treated with fractions and isolated flavonoids of Spirodela polyrhiza. An ethanol extract of S. polyrhiza was fractionated into three fractions. The butanol soluble fraction (SPB) exhibited potent antiadipogenesis activity and decreased C/EBPα and PPARγ protein expression level in 3T3‐L1 cells without significant cytotoxicity. The flavonoids were isolated from SPB and their chemical structures were identified as chrysoeriol (1), apigenin (2), luteolin (3), vitexin (4), cosmosin (5), orientin (6) and luteolin‐7‐O‐β‐d‐glucoside (7) by spectroscopic analysis. Studies on the adipogenesis and intracellular triglyceride accumulation inhibitory effect showed that compounds 4 and 6 had the highest activity and decreased C/EBPα and PPARγ protein expression level in 3T3‐L1 cells. These results suggest that the flavonoids isolated from SPB, especially compounds 4 and 6, contribute to the inhibitory activity of S. polyrhiza in 3T3‐L1 cells. Copyright © 2010 John Wiley & Sons, Ltd.

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Phytotherapy Research

Tập 24 Số 10

1543-1548

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