Vitamin D3 compounds regulate human immunodeficiency virus type 1 replication in U937 monoblastoid cells and in monocyte-derived macrophages

Journal of Leukocyte Biology - Tập 53 Số 2 - Trang 157-164 - 1993
C. David Pauza1, Richard S. Kornbluth2, Peter Emau1, Douglas D. Richman2,3, Leonard J. Deftos4,3
1Department of Pathology and Laboratory Mediane, University of Wisconsin , Madison, San Diego, California
2Departments of Pathology and Mediane, University of California , San Diego, California
3San Diego Veterans Administration Medical Center, San Diego, California
4Department of Mediane, University of California , San Diego, California

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Abstract We studied the effects of vitamin D3 compounds on the replication of human immunodeficiency virus type 1 (HIV-1) in the monoblastoid cell line U937 and in primary monocyte-derived macrophage cultures to understand how modulators of monocyte/macrophage effector function might affect the pathogenesis of HIV-1 infection. U937 cell cultures exposed to 1,α25-dihydro- xyvitamin D3 prior to HIV-1 infection showed enhanced virus replication that was apparently due to increased cellular resistance to viral cytopathic effects; a marked inhibition of virus replication was noted in cells exposed to 1,α25-dihydroxyvitamin D3 subsequent to infection. Exposure of blood-derived monocyte/macrophages to vitamin D3 compounds prior to infection also affected virus growth; in most cases, substantial inhibition of HIV- 1 replication was noted in vitamin D3-treated macrophage cultures. Our results demonstrate that vitamin D3 compounds with recognized abilities to induce cellular differentiation can modulate HIV-1 infection of human macrophages. J. Leukoc. Biol. 53: 157–164; 1993.

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Journal of Leukocyte Biology

Tập 53 Số 2

157-164

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