Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging

Proteomics - Tập 16 Số 11-12 - Trang 1742-1746 - 2016
Matthias Holzlechner1, Sonja Reitschmidt1, Sabine Gruber2, Susanne Zeilinger3,2, Martina Marchetti‐Deschmann1
1Institute of Chemical Technologies and Analytics, Vienna University of Technology, Vienna, Austria
2Institute of Microbiology, University of Innsbruck, Innsbruck, Austria
3Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria

Tóm tắt

Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home‐built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.

Từ khóa


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Thông tin
Thông tin xuất bản

Proteomics

Tập 16 Số 11-12

1742-1746

Thông tin tác giả