Phát hiện cysteine bằng phương pháp màu sắc khả kiến dựa trên vật liệu ZIF-67 biến đổi nano vàng

Chemical Papers - Tập 74 - Trang 1839-1847 - 2019
Li Liu1, Yan-jun Hao1,2, Zhen Li1, Cheng Chen1, Ming-yu Wu1, Shun Feng1
1School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, China
2Hohhot Food Inspection Institute, Hohhot Food and Drug Administration, Hohhot, China

Tóm tắt

Một vật liệu khung imidazolate zeolitic (ZIF-67) đã được biến đổi bằng nano vàng (Au@ZIF-67) được chuẩn bị từ oxit cobalt(II). Hình thái, thành phần nguyên tố và tính chất màu sắc của nó đã được xác định bằng máy quang phổ UV–Vis, kính hiển vi quét phát xạ trường, kính hiển vi điện tử truyền qua và phổ tán xạ năng lượng. Trong sự hiện diện của 20 axit amin cấu tạo nên protein, độ hấp thụ đặc trưng của dung dịch Au@ZIF-67 tại 592 nm giảm đi tương ứng chỉ trong sự hiện diện của cysteine (Cys) và gần như không thay đổi khi thêm 19 axit amin còn lại vào hoặc trộn lẫn. Do đó, cả phương pháp quan sát bằng mắt thường và phương pháp quang phổ khả kiến được đề xuất cho việc phát hiện Cys với giới hạn phát hiện lần lượt là 0.5 µM và 0.1 µM. Sự thay đổi màu sắc khác biệt, thời gian phản ứng ngắn, độ đặc hiệu và độ nhạy cao đã khiến Au@ZIF-67 trở thành một cảm biến màu sắc khả kiến đầy hứa hẹn cho Cys trong nước. Hơn nữa, cơ chế tiềm năng đã được nghiên cứu. Kết quả chỉ ra rằng các hạt nano Au đóng vai trò chính.

Từ khóa

#cystein #nano vàng #ZIF-67 #phương pháp quang phổ #cảm biến màu sắc

Tài liệu tham khảo

Ahmed KBA, Sengan M, Kumar PS, Veerappan A (2016) Highly selective colorimetric cysteine sensor based on the formation of cysteine layer on copper nanoparticles. Sens Actuators B 233:431–437. https://doi.org/10.1016/j.snb.2016.04.125 Banerjee R, Phan A, Wang B, Knobler C, Furukawa H, O’Keeffe M, Yaghi OM (2008) High-throughput synthesis of zeolitic imidazolate frameworks and application to CO2 capture. Science 319:939–943. https://doi.org/10.1126/science.1152516 Burford N, Eelman MD, Mahony DE, Morash M (2002) Definitive identification of cysteine and glutathione complexes of bismuth by mass spectrometry: assessing the biochemical fate of bismuth pharmaceutical agents. Chem Commun 9:146–147. https://doi.org/10.1039/b210570e Chen G, Zhang L, Wang J (2004) Miniaturized capillary electrophoresis system with a carbon nanotube microelectrode for rapid separation and detection of thiols. Talanta 64:1018–1023. https://doi.org/10.1016/j.talanta.2004.04.022 Chen X, Zhou Y, Peng X, Yoon J (2010) Fluorescent and colorimetric probes for detection of thiols. Chem Soc Rev 39:2120–2135. https://doi.org/10.1039/b925092a Dobrotvorskaya IS, Fedorova TN, Dobrota D, Berezov TT (2011) Characteristics of oxidative stress in experimental rat brain ischemia aggravated by homocysteic acid. Neurochem J 5:42–46. https://doi.org/10.1134/S1819712410041014 Fei S, Chen J, Yao S, Deng G, He D, Kuang Y (2005) Electrochemical behavior of l-cysteine and its detection at carbon nanotube electrode modified with platinum. Anal Biochem 339:29–35. https://doi.org/10.1016/j.ab.2005.01.002 Go YM, Jones DP (2011) Cysteine/cystine redox signaling in cardiovascular disease. Free Radic Biol Med 50:495–509. https://doi.org/10.1016/j.freeradbiomed.2010.11.029 Goodman MT, Mcduffie K, Hernandez B, Wilkens LR, Selhub J (2000) Case-control study of plasma folate, homocysteine, vitamin B12, and cysteine as markers of cervical dysplasia. Cancer 89:376–382. https://doi.org/10.1002/1097-0142(20000715)89:2%3c376:AID-CNCR24%3e3.0.CO;2-O Hai X, Lin X, Chen X, Wang J (2018) Highly selective and sensitive detection of cysteine with a graphene quantum dots-gold nanoparticles based core-shell nanosensor. Sens Actuators B 257:228–236. https://doi.org/10.1016/j.snb.2017.10.169 Han C, Xu K, Liu Q, Liu X, Li J (2014) Colorimetric sensing of cysteine using label-free silver nanoparticles. Sens Actuators B 202:574–582. https://doi.org/10.1016/j.snb.2014.05.139 Han B, Hu X, Yan Q, Jiang J, He G (2019) Ag-location-based color-tunable fluorescent AuAg nanoclusters for “turn-on” and “turn-off” detection of l-cysteine. Sens Actuators B 284:695–703. https://doi.org/10.1016/j.snb.2019.01.014 Inoue T, Kirchhoff JR (2002) Determination of thiols by capillary electrophoresis with amperometric detection at a coenzyme pyrroloquinoline quinone modified electrode. Anal Chem 74:1349–1354. https://doi.org/10.1021/ac0108515 Ivanov AR, Nazimov IV, Baratova L, Lobazov AP, Popkovich GB (2001) Determination of biologically active low-molecular-mass thiols in human blood: III. Highly sensitive narrow-bore isocratic reversed-phase high-performance liquid chromatography with fluorescence detection. J Chromatogr A 913:315–318. https://doi.org/10.1016/S0021-9673(00)00993-6 Javad S et al (2011) Serum homocystein levels in cardiovascular disease. Clin Biochem 44:S187–S187. https://doi.org/10.1016/j.clinbiochem.2011.08.956 Jiang Y, Yang QM, Xu QJ, Lu SY, Hu LY, Xu MW, Liu YS (2019) Metal organic framework MIL-53(Fe) as an efficient artificial oxidase for colorimetric detection of cellular biothiols. Anal Biochem 577:82–88. https://doi.org/10.1016/j.ab.2019.04.020 Johnson JM, Strobel FH, Reed M, Pohl J, Jones DP (2008) A rapid LC-FTMS method for analysis of cysteine, cystine and cysteine/cystine steady-stateredox potential in human plasma. Clin Chim Acta 396:43–48. https://doi.org/10.1016/j.cca.2008.06.020 Kubalczyk P, Bald E, Furmaniak P, Glowacki R (2014) Simultaneous determination of total homocysteine and cysteine in human plasma by capillary zone electrophoresis with pH-mediated sample stacking. Anal Methods 6:4138–4143. https://doi.org/10.1039/c4ay00287c Lawrence NS, Deo RP, Wang J (2004) Detection of homocysteine at carbon nanotube paste electrodes. Talanta 63:443–449. https://doi.org/10.1016/j.talanta.2003.11.024 Li YS, Liang FY, Bux H, Feldhoff A, Yang WS, Caro J (2010) Molecular sieve membrane: supported metal-organic framework with high hydrogen selectivity. Angew Chem-Int Edit 49:548–551. https://doi.org/10.1002/anie.200905645 Li J et al (2013) Copper(II) imidazolate frameworks as highly efficient photocatalysts for reduction of CO2 into methanol under visible light irradiation. J Solid State Chem 203:154–159. https://doi.org/10.1016/j.jssc.2013.04.016 Li JJ, Qiao D, Zhao J, Weng GJ, Zhu J, Zhao JW (2019) Fluorescence turn-on sensing of l-cysteine based on FRET between Au–Ag nanoclusters and Au nanorods. Spectrochim Acta Part A Mol Biomol Spectrosc 217:247–255. https://doi.org/10.1016/j.saa.2019.03.092 Lima PR, Santos WJR, Luz RDCS, Damos FS, Oliveira AB, Goulart MOF, Kubota LT (2008) An amperometric sensor based on electrochemically triggered reaction: redox-active Ar–NO/Ar–NHOH from 4-nitrophthalonitrile-modified electrode for the low voltage cysteine detection. J Electroanal Chem 612:87–96. https://doi.org/10.1016/j.jelechem.2007.09.013 Lin KYA, Chang HA (2015) Zeolitic Imidazole Framework-67 (ZIF-67) as a heterogeneous catalyst to activate peroxymonosulfate for degradation of Rhodamine B in water. J Taiwan Inst Chem Eng 53:40–45. https://doi.org/10.1016/j.jtice.2015.02.027 Meng W, Wen Y, Dai L, He Z, Wang L (2018) A novel electrochemical sensor for glucose detection based on Ag@ZIF-67 nanocomposite. Sens Actuators B 260:852–860. https://doi.org/10.1016/j.snb.2018.01.109 Nolin TD, Mcmenamin ME, Himmelfarb J (2007) Simultaneous determination of total homocysteine, cysteine, cysteinylglycine, and glutathione in human plasma by high-performance liquid chromatography: application to studies of oxidative stress. J Chromatogr B 852:554–561. https://doi.org/10.1016/j.jchromb.2007.02.024 Ogasawara Y, Mukai Y, Togawa T, Suzuki T, Tanabe S, Ishii K (2007) Determination of plasma thiol bound to albumin using affinity chromatography and high-performance liquid chromatography with fluorescence detection: ratio of cysteinyl albumin as a possible biomarker of oxidative stress. J Chromatogr B 845:157–163. https://doi.org/10.1016/j.jchromb.2006.08.006 Park KS et al (2006) Exceptional chemical and thermal stability of zeolitic imidazolate frameworks. Proc Natl Acad Sci USA 103:10186–10191. https://doi.org/10.1073/pnas.0602439103 Potesil D et al (2005) Simultaneous femtomole determination of cysteine, reduced and oxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high-performance liquid chromatography with electrochemical detection. J Chromatogr A 1084:134–144. https://doi.org/10.1016/j.chroma.2005.06.019 PukaSundvall M, Eriksson P, Nilsson M, Sandberg M, Lehmann A (1995) Neurotoxicity of cysteine: interaction with glutamate. Brain Res 705:65–70. https://doi.org/10.1016/0006-8993(95)01139-0 Qian Q, Deng J, Wang D, Yang L, Yu P, Mao L (2012) Aspartic acid-promoted highly selective and sensitive colorimetric sensing of cysteine in rat brain. Anal Chem 84:9579–9584. https://doi.org/10.1021/ac3024608 Rafii M, Elango R, Courtney-Martin G, House JD, Fisher L, Pencharz PB (2007) High-throughput and simultaneous measurement of homocysteine and cysteine in human plasma and urine by liquid chromatography-electrospray tandem mass spectrometry. Anal Biochem 371:71–81. https://doi.org/10.1016/j.ab.2007.07.026 Shahrokhian S (2001) Lead phthalocyanine as a selective carrier for preparation of a cysteine-selective electrode. Anal Chem 73:5972–5978. https://doi.org/10.1021/ac010541m Shin IS, Gwon SY, Kim SH (2014) A specific colorimetric signaling of biological thiolsbased on intermolecular n-π charge transfer interaction. Fibers Polym 15:891–893. https://doi.org/10.1007/s12221-014-0891-7 Tcherkas YV, Denisenko AD (2001) Simultaneous determination of several amino acids, including homocysteine, cysteine and glutamic acid, in human plasma by isocratic reversed-phase high-performance liquid chromatography with fluorimetric detection. J Chromatogr A 913:309–313. https://doi.org/10.1016/S0021-9673(00)01201-2 Tian H, Fan H, Li M, Ma L (2015) Zeolitic imidazolate framework coated ZnO nanorods as molecular sieving to improve selectivity of formaldehyde gas sensor. ACS Sens 1:243–250. https://doi.org/10.1021/acssensors.5b00236 Vellasco AP, Haddad R, Eberlin MN, Höehr NF (2002) Combined cysteine and homocysteine quantitation in plasma by trap and release membrane introduction mass spectrometry. Analyst 127:1050–1053. https://doi.org/10.1039/B203832C Wang W et al (2005) Detection of homocysteine and cysteine. J Am Chem Soc 127:15949–15958. https://doi.org/10.1021/ja054962n Wang L, Xu R, Hu B, Li W, Sun Y, Tu Y, Zeng X (2010) Analysis of free amino acids in Chinese teas and flower of tea plant by high performance liquid chromatography combined with solid-phase extraction. Food Chem 123:1259–1266. https://doi.org/10.1016/j.foodchem.2010.05.063 Wen XF, Li CC, Meng FC (2014) Synthesis and Characterization of CePO4 nanorods via solvothermal process. J Wuhan Univ Technol Mater Sci Ed 29:229–232. https://doi.org/10.1007/s11595-014-0898-3 Wu LL, Wang LY, Xie ZJ, Pan N, Peng CF (2016) Colorimetric assay of l-cysteine based on peroxidase-mimicking DNA-Ag/Pt nanoclusters. Sens Actuators B 235:110–116. https://doi.org/10.1016/j.snb.2016.04.125 Xiao W, Hu H, Huang J (2012) Colorimetric detection of cysteine by surface functionalization of natural cellulose substance. Sens Actuators B 171–172:878–885. https://doi.org/10.1016/j.snb.2012.05.087 Xu S, Wang Y, Sun Y, Shan G, Chen Y, Liu Y (2017) The detection of copper ions based on photothermal effect of cysteine modified Au nanorods. Sens Actuators B 248:761–768. https://doi.org/10.1016/j.snb.2017.04.016 Yang H, He XW, Wang F, Kang Y, Zhang J (2012) Doping copper into ZIF-67 for enhancing gas uptake capacity and visible-light-driven photocatalytic degradation of organic dye. J Mater Chem 22:21849–21851. https://doi.org/10.1039/c2jm35602c Zhang J, Xu X, Yuan Y, Yang C, Yang X (2011) A Cu@Au nanoparticle-based colorimetric competition assay for the detection of sulfide anion and cysteine. ACS Appl Mater Interfaces 3:2928–2931. https://doi.org/10.1021/am2007678 Zhao XD et al (2018) Design of “turn-on” fluorescence sensor for l-cysteine based on the instability of metal-organic frameworks. Microporous Mesoporous Mat 268:88–92. https://doi.org/10.1016/j.micromeso.2018.04.019