View on a mechanistic model of Chlorella vulgaris in incubated shake flasks

Bioprocess and Biosystems Engineering - Tập 45 - Trang 15-30 - 2021
Fabian Kuhfuß1, Veronika Gassenmeier1, Sahar Deppe2, George Ifrim3, Tanja Hernández Rodríguez1, Björn Frahm1
1Biotechnology and Bioprocess Engineering, Ostwestfalen-Lippe University of Applied Sciences and Arts, Lemgo, Germany
2Fraunhofer IOSB, Lemgo, Germany
3Dunarea de Jos University of Galati, Galati, Romania

Tóm tắt

Kinetic growth models are a useful tool for a better understanding of microalgal cultivation and for optimizing cultivation conditions. The evaluation of such models requires experimental data that is laborious to generate in bioreactor settings. The experimental shake flask setting used in this study allows to run 12 experiments at the same time, with 6 individual light intensities and light durations. This way, 54 biomass data sets were generated for the cultivation of the microalgae Chlorella vulgaris. To identify the model parameters, a stepwise parameter estimation procedure was applied. First, light-associated model parameters were estimated using additional measurements of local light intensities at differ heights within medium at different biomass concentrations. Next, substrate related model parameters were estimated, using experiments for which biomass and nitrate data were provided. Afterwards, growth-related model parameters were estimated by application of an extensive cross validation procedure.

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