Variation of lysosomal enzyme activity with gestational age in chorionic villi
Tóm tắt
The activities of 14 lysosomal enzymes in chorionic villi at gestational ages of 6–12 weeks were assayed. Arylsulphatases A and B, α-glucosidase and β-glucuronidase activities increased with advancing gestational age. When compared with the activity in cultured amniotic fluid cells, arylsulphatase A, β-galactosidase, α-glucosidase, heparan N-sulphatase, α-l-iduronidase, α-mannosidase, neuraminidase and sphingomyelinase showed significant differences. All except β-glucuronidase showed lower activity in chorionic villi than in cultured amniotic fluid cells. Prenatal diagnosis using chorionic villi was possible except for α-l-iduronidase. Storage at −20°C up to 42 days did not significantly affect activity. The results emphasize the importance of using fresh or frozen age-matched control tissue for diagnosis.
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