Xác thực các gen tham chiếu cho phân tích biểu hiện định lượng bằng phản ứng chuỗi polymerase thời gian thực (RT-PCR) trong Saccharomyces cerevisiae
Tóm tắt
RT-PCR thời gian thực là phương pháp được khuyến nghị cho phân tích biểu hiện gen định lượng. Một bước bắt buộc là chọn các gen tham chiếu tốt để chuẩn hóa. Một vài gen thường được gọi là gen HouseKeeping (HSK), chẳng hạn như
Từ các tập dữ liệu vi điều hòa công khai, chúng tôi đã chọn các gen tham chiếu tiềm năng mà biểu hiện của chúng rõ ràng vẫn không thay đổi trong quá trình phát triển lâu dài trên glucose. Sử dụng thuật toán geNorm,
Trong công việc này, chúng tôi đã cung cấp một tập hợp các gen phù hợp làm gen tham chiếu cho phân tích biểu hiện gen định lượng bằng RT-PCR thời gian thực trên các mẫu sinh học men bao gồm một bảng lớn các trạng thái sinh lý. Ngược lại, chúng tôi đã không xác nhận và không khuyến khích việc sử dụng
Từ khóa
#RT-PCR #gene biểu hiện #thực vật #Saccharomyces cerevisiae #chuẩn hóa gen #gen tham chiếuTài liệu tham khảo
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