Using microarrays and functional genomics to understand and target cancer
Tóm tắt
In our work microarrays are of importance for screening the whole genome to sort out important genes and also for constructing pathways systems for the understanding of the links between genes. Our studies demonstrate the potential clinical usefulness of microarrays for the classification of patients and for understanding cancer biology and identifying new drug targets.
Tài liệu tham khảo
Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Orntoft T, Lukas J, Bartek J. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature. 2005 Apr 14;434(7035):864–870.
Koed K, Wiuf C, Christensen LL, Wikman FP, Zieger K, Moller K, von der Maase H, Orntoft TF. High-density single nucleotide polymorphism array defines novel stage and location-dependent allelic imbalances in human bladder tumors. Cancer Res. 2005 Jan 1;65(1):34–45.
Olesen SH, Christensen LL, Sorensen FB, Cabezon T, Laurberg S, Orntoft TF, Birkenkamp-Demtroder K. Human FK 506 binding protein 65 is associated with colorectal cancer. Mol Cell Proteomics. 2005 Apr;4(4):534–44. Epub 2005 Jan 25.
Dyrskjot L, Thykjaer T, Kruhoffer M, Jensen JL, Marcussen N, Hamilton-Dutoit S, Wolf H, Orntoft TF. Identifying distinct classes of bladder carcinoma using microarrays. Nat Genet. 2003 Jan;33(1):90–96.
De Vos J, Thykjaer T, Tarte K, Ensslen M, Raynaud P, Requirand G, Pellet F, Pantesco V, Reme T, Jourdan M, Rossi JF, Orntoft T, Klein B. Comparison of gene expression profiling between malignant and normal plasma cells with oligonucleotide arrays. Oncogene. 2002 Oct 3;21(44):6848–6857.
Spicker JS, Wikman F, Lu ML, Cordon-Cardo C, Workman C, ORntoft TF, Brunak, Knudsen S. Neural network predicts sequence of TP53 gene based on DNA chip. Bioinformatics. 2002 Aug;18(8):1133–1134.
Orntoft TF, Thykjaer T, Waldman FM, Wolf H, Celis JE. Genome-wide study of gene copy numbers, transcripts, and protein levels in pairs of non-invasive and invasive human transitional cell carcinomas. Mol Cell Proteomics. 2002 Jan;1(1):37–45.
Liang G, Gonzales FA, Jones PA, Orntoft TF, Thykjaer T. Analysis of gene induction in human fibroblasts and bladder cancer cells exposed to the methylation inhibitor 5-aza-2′-deoxycytidine. Cancer Res. 2002 Feb 15;62(4):961–966.
Primdahl H, Wikman FP, von der Maase H, Zhou XG, Wolf H, Orntoft TF. Allelic imbalances in human bladder cancer: genome-wide detection with high-density single-nucleotide polymorphism arrays. J Natl Cancer Inst. 2002 Feb 6;94(3):216–223.
Thykjaer T, Christensen M, Clark AB, Hansen LR, Kunkel TA, Orntoft TF. Functional analysis of the mismatch repair system in bladder cancer. Br J Cancer. 2001 Aug 17;85(4):568–575.
Olesen SH, Thykjaer T, Orntoft TF. Mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in human bladder cancer, screening for mutations and loss of heterozygosity. Carcinogenesis. 2001 May;22(5):813–815.
Thykjaer T, Workman C, Kruhoffer M, Demtroder K, Wolf H, Andersen LD, Frederiksen CM, Knudsen S, Orntoft TF. Identification of gene expression patterns in superficial and invasive human bladder cancer. Cancer Res. 2001 Mar 15;61(6):2492–2499.
Primdahl H, von der Maase H, Christensen M, Wolf H, Orntoft TF. Allelic deletions of cell growth regulators during progression of bladder cancer. Cancer Res. 2000 Dec 1;60(23):6623–6629.
Wikman FP, Lu ML, Thykjaer T, Olesen SH, Andersen LD, Cordon-Cardo C, Orntoft TF. Evaluation of the performance of a p53 sequencing microarray chip using 140 previously sequenced bladder tumor samples. Clin Chem. 2000 Oct;46(10):1555–1561.
Celis JE, Kruhoffer M, Gromova I, Frederiksen C, Ostergaard M, Thykjaer T, Gromov P, Yu J, Palsdottir H, Magnusson N, Orntoft TF. Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics. FEBS Lett. 2000 Aug 25;480(1):2–16. Review.