Use of combined conventional and real-time PCR to determine the epidemiology of feline haemoplasma infections in northern Italy

Journal of Feline Medicine and Surgery - Tập 11 - Trang 277-285 - 2009
Fabio Gentilini1,2, Marilisa Novacco1, Maria E. Turba2, Barbara Willi3, Maria L. Bacci2, Regina Hofmann-Lehmann3
1Veterinary Clinical Department, University of Bologna, Ozzano dell’Emilia, Bologna, Italy
2DIMORFIPA, University of Bologna, Ozzano dell'Emilia, Bologna, Italy
3Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland

Tóm tắt

Although knowledge of feline haemotropic mycoplasmas (haemoplasmas) has dramatically improved in recent years, some issues still remain to be elucidated. The aim of the current study was to evaluate the prevalence of feline haemoplasma infections in blood samples collected from cats in northern Italy. A convenience-sample of 307 cats (40 anaemic; 258 non-anaemic; nine with unknown haematocrit [HCT]) was investigated using polymerase chain reaction assays. Furthermore, the date of blood collection, signalment and clinicopathological data were retrospectively evaluated to assess predictors and risk factors for infection. Haemoplasma infections were highly prevalent in the sample investigated with an overall prevalence of 18.9% (95% confidence interval: 14.5–23.3%). The prevalence for the three feline haemoplasmas was 17.3% for ‘ Candidatus Mycoplasma haemominutum’ ( CMhm), 5.9% for Mycoplasma haemofelis ( Mhf) and 1.3% for ‘ Candidatus Mycoplasma turicensis’ ( CMt). Feline immunodeficiency virus-positive status represented a risk factor for infection with an odds ratio of 4.19 ( P=0.02). Moreover, a higher prevalence was observed in summer (odds ratio 1.78; P=0.04) which may be consistent with arthropod-borne disease transmission. Cats infected with Mhf showed significantly lower HCT ( P=0.03), haemoglobin values ( P=0.02) and red blood cell counts ( P=0.04), lower mean corpuscular haemoglobin concentration ( P<0.01) and higher white blood cell counts ( P<0.01) when compared with non-infected cats.

Tài liệu tham khảo

Jain N.C., 1973, Am J Vet Res, 34, 697 10.1128/JCM.02005-06 Berent L.M., 1998, Am J Vet Res, 59, 1215 Foley J.E., 1998, Am J Vet Res, 59, 1581 10.1128/JCM.36.2.462-466.1998 10.1099/00207713-51-3-815 10.1099/00207713-51-3-891 10.1128/JCM.43.6.2581-2585.2005 10.1111/j.1939-1676.2007.tb03009.x 10.2460/ajvr.2001.62.687 10.1016/j.vetmic.2006.06.015 10.1016/j.micinf.2005.08.015 10.1016/j.jfms.2007.12.004 10.2460/ajvr.2001.62.604 10.1136/vr.152.7.193 10.1111/j.1751-0813.2002.tb11306.x Harrus S., 2002, Vet Res, 151, 82 10.1016/S0378-1135(03)00044-0 10.1292/jvms.66.1017 10.1016/j.jfms.2003.12.003 10.1016/j.jfms.2003.11.005 10.1016/j.jfms.2005.12.002 10.1016/j.jfms.2005.08.003 10.1128/JCM.44.3.961-969.2006 10.1128/JCM.00987-06 10.1136/vr.119.15.373 Grindem C.B., 1990, J Am Vet Med Assoc, 196, 96 10.1136/vr.122.2.32 10.2460/ajvr.2002.63.1172 Just F., 2007, Berl Munch Tierarztl Wochenschr, 120, 197 Flint J.C., 1958, Am J Vet Res, 70, 164 10.1016/j.jfms.2006.04.005 10.1016/j.vetmic.2004.06.013 10.1292/jvms.67.1277 10.2460/ajvr.2005.66.1008 10.1128/AEM.02977-06 10.2460/ajvr.67.3.494 10.1016/j.jfms.2007.12.007 10.1093/nar/gkm234 Sukura A., 1992, Acta Vet Scand, 33, 325, 10.1186/BF03547298 10.1128/JCM.41.1.439-441.2003 10.1016/j.ijpara.2006.06.014 10.1111/j.1748-5827.1973.tb06422.x Pennisi M.G., 1994, Veterinaria, 8, 9 Raubel G.H., 1994, J Acquir Immune Defic Syndr, 7, 1003 10.5326/0400423
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Journal of Feline Medicine and Surgery

Tập 11

277-285

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