Uroplakin IIIb, a urothelial differentiation marker, dimerizes with uroplakin Ib as an early step of urothelial plaque assembly

Journal of Cell Biology - Tập 159 Số 4 - Trang 685-694 - 2002
Fang‐Ming Deng1, Feng‐Xia Liang1, Liyu Tu2, Katheryn A. Resing3, Ping Hu1, Mark Supino2, Chih‐Chi Andrew Hu1,4, Ge Zhou1,4, Mingxiao Ding5, Gert Kreibich2,6, Tung‐Tien Sun1,4,7,6
11Epithelial Biology Unit, Ronald O. Perelman Department of Dermatology
22Department of Cell Biology, New York University School of Medicine, New York, NY 10016
36Department of Chemistry, University of Colorado, Boulder, CO 80309
43Department of Pharmacology, New York University School of Medicine, New York, NY 10016
57Department of Cell Biology, College of Life Sciences, Peking University, Beijing, People's Republic of China 100871
65New York University Cancer Institute, New York University School of Medicine, New York, NY 10016
74Department of Urology, New York University School of Medicine, New York, NY 10016

Tóm tắt

Urothelial plaques consist of four major uroplakins (Ia, Ib, II, and III) that form two-dimensional crystals covering the apical surface of urothelium, and provide unique opportunities for studying membrane protein assembly. Here, we describe a novel 35-kD urothelial plaque-associated glycoprotein that is closely related to uroplakin III: they have a similar overall type 1 transmembrane topology; their amino acid sequences are 34% identical; they share an extracellular juxtamembrane stretch of 19 amino acids; their exit from the ER requires their forming a heterodimer with uroplakin Ib, but not with any other uroplakins; and UPIII-knockout leads to p35 up-regulation, possibly as a compensatory mechanism. Interestingly, p35 contains a stretch of 80 amino acid residues homologous to a hypothetical human DNA mismatch repair enzyme-related protein. Human p35 gene is mapped to chromosome 7q11.23 near the telomeric duplicated region of Williams-Beuren syndrome, a developmental disorder affecting multiple organs including the urinary tract. These results indicate that p35 (uroplakin IIIb) is a urothelial differentiation product structurally and functionally related to uroplakin III, and that p35–UPIb interaction in the ER is an important early step in urothelial plaque assembly.

Từ khóa


Tài liệu tham khảo

1979, Pediatr. Radiol., 8, 29, 10.1007/BF00973674

1998, Cancer Res., 58, 1087

1998, Hum. Mol. Genet., 7, 887, 10.1093/hmg/7.5.887

1994, Radiology., 190, 695, 10.1148/radiology.190.3.8115613

1983, J. Mol. Biol., 166, 21, 10.1016/S0022-2836(83)80048-5

2001, Proc. Natl. Acad. Sci. USA., 98, 154, 10.1073/pnas.98.1.154

1996, Proc. Natl. Acad. Sci. USA., 93, 6025, 10.1073/pnas.93.12.6025

1996, Hum. Mol. Genet., 5, 1893, 10.1093/hmg/5.12.1893

1999, Science., 286, 1882, 10.1126/science.286.5446.1882

1999, Hum. Mol. Genet., 8, 1947, 10.1093/hmg/8.10.1947

1999, J. Gen. Physiol., 113, 163, 10.1085/jgp.113.2.163

1995, N. Engl. J. Med., 332, 839, 10.1056/NEJM199503303321302

2000, Annu. Rev. Genet., 34, 359, 10.1146/annurev.genet.34.1.359

2001, J. Cell Biol., 155, 1103, 10.1083/jcb.200108061

1969, Nature., 224, 1304, 10.1038/2241304a0

1999, Genomics., 58, 138, 10.1006/geno.1999.5815

1994, Biochem. Biophys. Res. Commun., 204, 1257, 10.1006/bbrc.1994.2598

2000, J. Cell Biol., 151, 961, 10.1083/jcb.151.5.961

1999, J. Mol. Biol., 285, 595, 10.1006/jmbi.1998.2304

1984, Nucleic Acids Res., 12, 857, 10.1093/nar/12.2.857

2000, Genomics., 70, 396, 10.1006/geno.2000.6412

1999, Clin. Pediatr. (Phila.)., 38, 189, 10.1177/000992289903800401

1999, Differentiation., 65, 59, 10.1046/j.1432-0436.1999.6510059.x

2001, Biochem. J., 355, 13, 10.1042/bj3550013

1994, J. Biol. Chem., 269, 1775, 10.1016/S0021-9258(17)42095-3

1995, Proc. Natl. Acad. Sci. USA., 92, 679, 10.1073/pnas.92.3.679

1997, FASEB J., 11, 428, 10.1096/fasebj.11.6.9194523

1996, Annu. Rev. Biochem., 65, 101, 10.1146/annurev.bi.65.070196.000533

1990, Am. J. Med. Genet. Suppl., 6, 102

1994, Nature., 371, 75, 10.1038/371075a0

1995, Genomics., 30, 195, 10.1006/geno.1995.9885

1996, Am. J. Med. Genet., 63, 301, 10.1002/(SICI)1096-8628(19960503)63:1<301::AID-AJMG49>3.0.CO;2-P

2000, Am. J. Hum. Genet., 66, 47, 10.1086/302722

1996, Am. J. Hum. Genet., 59, 781

1994, Science., 265, 1091, 10.1126/science.8066446

1998, Bioessays., 20, 546, 10.1002/(SICI)1521-1878(199807)20:7<546::AID-BIES5>3.0.CO;2-I

1993, Biochemistry., 32, 10036, 10.1021/bi00089a020

2001, Virchows Arch., 438, 181, 10.1007/s004280000315

1977, Proc. Natl. Acad. Sci. USA., 74, 5463, 10.1073/pnas.74.12.5463

1996, J. Pediatr., 129, 466, 10.1016/S0022-3476(96)70086-0

1996, Anal. Chem., 68, 850, 10.1021/ac950914h

1999, Adv. Exp. Med. Biol., 462, 7, 10.1007/978-1-4615-4737-2_1

1990, J. Cell Sci, 97, 419, 10.1242/jcs.97.3.419

2002, Mol. Biol. Cell., In press

1996, Am. J. Hum. Genet., 59, 958

2000, Genomics, 69, 1, 10.1006/geno.2000.6312

1969, J. Mol. Biol., 46, 593, 10.1016/0022-2836(69)90200-9

1995, J. Mol. Biol., 248, 887, 10.1006/jmbi.1995.0269

1993, J. Cell Sci., 106, 31, 10.1242/jcs.106.1.31

1990, J. Biol. Chem, 265, 19170, 10.1016/S0021-9258(17)30640-3

1995, J. Biol. Chem., 270, 29752, 10.1074/jbc.270.50.29752

1994, J. Cell Biol., 125, 171, 10.1083/jcb.125.1.171

Thông tin
Thông tin xuất bản

Journal of Cell Biology

Tập 159 Số 4

685-694

Thông tin tác giả