Urokinase gene 3′‐UTR T/C polymorphism is associated with oral cancer

Journal of Clinical Laboratory Analysis - Tập 18 Số 5 - Trang 276-279 - 2004
Ming‐Hsui Tsai1, Wen‐Chi Chen2,3, Huey‐Yi Chen2,4, Fuu‐Jen Tsai2,5
1Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan
2Department of Medical Genetics, China Medical University Hospital, Taichung, Taiwan
3Department of Urology, China Medical University Hospital, Taichung, Taiwan
4Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan
5Department of Pediatrics, China Medical University Hospital, Taichung, Taiwan

Tóm tắt

AbstractUrokinase is thought to be involved in the formation of oral cancer, although there is a lack of genetic evidence. Our aim was to study single nucleotide polymorphisms in order to investigate the possibility. A total of 130 oral cancer patients and 105 controls were studied. Polymerase chain reaction (PCR) based restriction analysis was used to identify the C/T polymorphism of the urokinase gene, which is located on the 3′‐untranslated region (3′‐UTR) of chromosome 10. There was a significant difference in the distribution of the urokinase gene 3′‐UTR C/T polymorphism frequency between cancer patients and the normal control group (P<0.05). The “T” allele was prominent in the cancer group. The odds ratio for the risk of the “T” allele in cancer patients was 2.71 (95% CI=1.325∼5.562). The cancer patients were further categorized according to gender and whether or not they were habitual smokers or betel nut chewers. These clinical parameters were then compared with tumor cell differentiation and tumor progression. No significant differences were found. Therefore, the urokinase gene 3′‐UTR “T” allele is associated with oral cancer and may play a role in oral cancer formation. However, we did not find the relationship between tumor progression and this polymorphism. J. Clin. Lab. Anal. 18:276–279, 2004. © 2004 Wiley‐Liss, Inc.

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Tài liệu tham khảo

10.1002/ijc.2910540113

10.1016/S0300-9785(84)80041-1

10.1016/S1368-8375(97)00028-6

10.1111/j.1600-0714.1995.tb01132.x

10.1038/bjc.1977.31

10.1177/00220345940730050501

Das BR, 2002, Understanding the biology of oral cancer, Med Sci Monit, 8, RA258

10.1073/pnas.82.13.4448

Conne B, 1997, Detection of polymorphism in the human urokinase‐type plasminogen activator gene, Thromb Hemost, 77, 434, 10.1055/s-0038-1655983

10.1002/(SICI)1097-0347(199710)19:7<611::AID-HED8>3.0.CO;2-A

10.1016/0003-9969(92)90082-J

Epstein JB, 2002, Advances in the diagnosis of oral premalignant and malignant lesions, J Can Dent Assoc, 68, 617

Sundqvist K, 1989, Cytotoxic and genotoxic effects of areca nut‐related compounds in cultured human buccal epithelial cells, Cancer Res, 49, 5294

10.1034/j.1600-0714.2001.030008458.x

Thông tin
Thông tin xuất bản

Journal of Clinical Laboratory Analysis

Tập 18 Số 5

276-279

Thông tin tác giả