Tổng hợp DNA không theo lịch trình trong tinh hoàn, một thử nghiệm thứ cấp để đánh giá các tác nhân gây đột biến hóa học

Fühner-Wieland's Sammlung von Vergiftungsfällen - Tập 46 - Trang 139-149 - 1980
G. Zbinden1
1Institut für Toxikologie, Eidgenössische Technische Hochschule und Universität Zürich, Schwerzenbach, Switzerland

Tóm tắt

Tổn thương DNA do methylmethane sulfonate, cyclophosphamide, doxorubicin và procarbazine gây ra trong các tế bào mầm đực đã được đánh giá ở thỏ thông qua việc chứng minh tổng hợp DNA không theo lịch trình (UDS) trong các giai đoạn chín giác quan và sau giác quan. Ngay sau khi điều trị qua đường tĩnh mạch, thymidine có gắn triti được tiêm vào cả hai tinh hoàn. Sau đó, thỏ đã được xuất tinh theo chuỗi, đầu tinh trùng được tách ra và thử nghiệm độ phóng xạ bằng phương pháp đếm scintillation lỏng. UDS phụ thuộc vào liều lượng đã được chứng minh ở các tế bào spermatocyte muộn và spermatid sớm. Liều cao của hycanthone cũng gây ra UDS, nhưng isoniazid và metronidazole không có tác dụng. Thử nghiệm UDS ở tinh hoàn thỏ xem xét các khía cạnh chuyển hóa và dược động học của các chất thử nghiệm và cung cấp thông tin về khả năng thâm nhập của chúng qua hàng rào máu-tinh hoàn. Do đó, thử nghiệm này hữu ích cho việc đánh giá thứ cấp các tác nhân gây đột biến tiềm năng. UDS do procarbazine gây ra đã bị loại bỏ khi điều trị đồng thời với Ara-C. Như vậy, bài thử nghiệm này cũng nhận diện các chất ức chế tổng hợp sửa chữa DNA.

Từ khóa

#DNA damage #methylmethane sulfonate #cyclophosphamide #doxorubicin #procarbazine #male germ cells #unscheduled DNA synthesis #testis #mutagens.

Tài liệu tham khảo

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