Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation

Plant Molecular Biology - Tập 57 - Trang 129-140 - 2005
Julie Castle1, Miklos Szekeres2, Gwen Jenkins1, Gerard J. Bishop1,3
1Institute of Biological Sciences, University of Wales, Aberystwyth, UK
2Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary
3Department of Agricultural Sciences, Imperial College London, Kent, UK

Tóm tắt

Brassinosteroids (BRs) are steroid hormones that are essential for plant growth and development. To gain insight into potential sites of BR synthesis, we studied promoter activities of the two Arabidopsis BR C-6 oxidase genes (CYP85A1 and CYP85A2) in transgenic plants carrying promoter fusions with the GUS, GFP or LUC reporter genes. BR-dependent feedback regulation of the GUS reporter constructs indicated that their expression corresponded to those of the native genes. Both the CYP85A1 and CYP85A2 promoters showed maximum activity during the first week following germination, particularly in the vascular tissues. Compared to CYP85A2, CYP85A1 expression was weaker and confined to the early stages of seedling development. Stronger CYP85A2 promoter activity was evident in both juvenile and adult plants. Comparison of the 5′-UTR and TATA box sequences of CYP85A1 and CYP85A2 revealed high homology, indicating a relatively recent gene duplication. We also found that transgenic Arabidopsis plants harbouring the tomato DWARF promoter-GUS fusion had similarities in the expression pattern to the Arabidopsis genes suggesting common transcriptional regulation of CYP85 genes in the two species.

Tài liệu tham khảo

T. Ait-Ali S. Frances J.L. Weller J.B. Reid R.E. Kendrick Y. Kamiya (1999) ArticleTitleRegulation of gibberellin 20-oxidase and gibberellin 3β-hydoxylase transcript accumulation during de-etiolation of pea seedlings Plant Physiol. 121 783–791

A.G. Arnim Particlevon X.W. Deng M.G. Stacey (1998) ArticleTitleCloning vectors for the expression of green fluorescent protein in transgenic plants Gene 221 35–43

M. Ashikari A. Sasaki M. Ueguchi-Tanaka H. Itoh A. Nishimura S. Datta K. Ishiyama T. Saito M. Kobayashi G.S. Khush H. Kitano M. Matsuoka (2002) ArticleTitleLoss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice ‘green revolution’ Breed. Sci. 52 143–150

S. Bancos T. Nomura T. Sato G. Molnar G.J. Bishop C. Koncz T. Yokota F. Nagy M. Szekeres (2002) ArticleTitleRegulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis Plant Physiol. 130 504–513

G.J. Bishop K. Harrison J.D.G. Jones (1996) ArticleTitleThe tomato Dwarf gene isolated by heterologous transposon tagging encodes the first member of a new cytochrome P450 family Plant Cell 8 959–969

G.J. Bishop C. Koncz (2002) ArticleTitleBrassinosteroids and plant steroid hormone signalling Plant Cell 14 S97–S110

G.J. Bishop T. Nomura T. Yokota K. Harrison T. Noguchi S. Fujioka S. Takatsuto J.D.G. Jones Y. Kamiya (1999) ArticleTitleThe tomato DWARF enzyme catalyses C-6 oxidation in brassinosteroid biosynthesis Proc. Natl. Acad. Sci. USA 96 1761–1766

G.J. Bishop T. Yokota (2001) ArticleTitlePlant steroid hormones, brassinosteroids: current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response Plant Cell Physiol. 42 114–120

M. Bradford (1976) ArticleTitleA rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 248–254 Occurrence Handle10.1006/abio.1976.9999 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D Occurrence Handle942051

N.K. Clay T. Nelson (2002) ArticleTitleVH1, a provascular cell-specific receptor kinase that influences leaf cell patterns in Arabidopsis Plant Cell 14 2707–2722

S.J. Clough A.F. Bent (1998) ArticleTitleFloral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Plant J. 16 735–743

S.D. Clouse K.A. Feldmann (1999) Molecular genetics of brassinosteroid action A. Sakurai T. Yokota S.D. Clouse (Eds) Brassinosteroids Steroidal Plant Hormones Springer Tokyo 163–190

S.D. Clouse J.M. Sasse (1998) ArticleTitleBrassinosteroids: essential regulators of plant growth and development Annu. Rev. Plant. Physiol. Plant Mol. Biol. 49 427–451

H. Fukuda (1997) ArticleTitleTracheary element differentiation Plant Cell 9 1147–1156

H. Goda Y. Shimada T. Asami S. Fujioka S. Yoshida (2002) ArticleTitleMicroarray analysis of brassinosteroid regulated genes in Arabidopsis thaliana Plant Physiol. 130 1319–1334

Z. Hong M. Ueguchi-Tanaka S. Shimizu-Sato Y. Inukai S. Fujioka Y. Shimada S. Takatsuto M. Agetsuma S. Yoshida Y. Watanabe S. Uozu H. Kitano M. Ashikari M. Matsuoka (2002) ArticleTitleLoss-of-function of a rice brassinosteroid biosynthetic enzyme, C-6 oxidase, prevents the organised arrangement and polar elongation of cells in the leaves and stem Plant J. 32 495–508

J.C. Jang S. Fujioka M. Tasaka H. Seto S. Takatsuto A. Ishii M. Aida S. Yoshida J. Sheen (2000) ArticleTitleA critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana Gene Dev. 14 1485–1497

R.A. Jefferson T.A. Kavanagh M.W. Bevan (1987) ArticleTitleGUS fusions: ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants EMBO J. 6 3901–3907

M. Kaneko H. Itoh Y. Inukai T. Sakamoto M. Ueguchi-Tanaka M. Ashikari M. Matsuoka (2003) ArticleTitleWhere do gibberellin biosynthesis and gibberellin signaling occur in rice plants? Plant J. 35 104–115

C. Koncz N. Martini L. Szabados M. Hrouda A. Brachmair J. Schell (1994) Specialized vectors for gene tagging and expression studies S.B. Gelvin A.R. Schilperoort (Eds) Plant Molecular Biology Manual NumberInSeriesVol. B2 Kluwer Academic Publishers Dordecht, The Netherlands 1–22

J.M. Li J. Chory (1997) ArticleTitleA putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction Cell 90 929–938 Occurrence Handle10.1016/S0092-8674(00)80357-8

J.M. Li J. Chory (1999) ArticleTitleBrassinosteroid action in plants J. Exp. Bot. 50 275–282

J.M. Li P. Nagpal V. Vitart T.C. McMorris J. Chory (1996) ArticleTitleA role for brassinosteroids in light-dependent development of Arabidopsis Science 272 398–401

B.N. Mandava (1988) ArticleTitlePlant growth-promoting brassinosteroids Annu. Rev. Plant Physiol. Plant Mol. Biol. 39 23–52

J. Mathur G. Molnar S. Fujioka S. Takatsuto A. Sakurai T. Yokota G. Adam B. Voigt F. Nagy C. Maas J. Schell C. Koncz M. Szekeres (1998) ArticleTitleTranscription of the Arabidopsis CPDgene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids Plant J. 14 593–602

T. Montoya T. Nomura K. Farrar T. Kaneta T. Yokota G.J. Bishop (2002) ArticleTitleCloning the tomato curl3 gene highlights the putative dual role of the leucine-rich repeat receptor kinase tBRI1/SR160 in plant steroid hormone and peptide hormone signaling Plant Cell 14 3163–3176

M. Mori T. Nomura H. Ooka M. Ishizaka T. Yokota K. Sugimoto K. Okabe H. Kajiwara K. Satoh K. Yamamoto H. Hirochika S. Kikuchi (2002) ArticleTitleIsolation and characterisation of a rice dwarf mutant defective in brassinosteroid biosynthesis Plant Physiol. 130 1152–1161

T. Murashige F. Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plantarum 15 473–497

T. Noguchi S. Fujioka S. Choe S. Takatsuto F.E. Tax S. Yoshida K.A. Feldmann (2000) ArticleTitleBiosynthetic pathways of brassinolide in Arabidopsis Plant Physiol. 124 201–209

S. Pien J. Wyrzykowska A.J. Fleming (2001) ArticleTitleNovel marker genes for early leaf development indicate spatial regulation of carbohydrate metabolism within the apical meristem Plant J. 25 663–674

K. Schrick U. Mayer A. Horrichs C. Kuhnt C. Bellini J. Dangl J. Schmidt G. Jürgens (2000) ArticleTitleFACKEL is a sterol C-14 reductase required for organised cell division and expansion in Arabidopsis embryogenesis Gene Dev. 14 1471–1484

M.A. Schuler D. Werck-Reichhart (2003) ArticleTitleFunctional genomics of P450s Annu. Rev. Plant Biol. 54 629–667

K. Schumacher J. Chory (2000) ArticleTitleBrassinosteroid signal transduction: still casting the actors Curr. Opin. Plant Biol. 3 79–84

Y. Shimada S. Fujioka N. Miyauchi M. Kushiro S. Takatsuto T. Nomura T. Yokota Y. Kamiya G.J. Bishop S. Yoshida (2001) ArticleTitleBrassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis Plant Physiol. 126 770–779

Y. Shimada H. Goda A. Nakamura S. Takatsuto S. Fujioka S. Yoshida (2003) ArticleTitleOrgan-specific expression of brassinosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis Plant Physiol. 131 287–297

S. Yamaguchi Y. Kamiya T.P. Sun (2001) ArticleTitleDistinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination Plant J. 28 443–453

R. Yamamoto S. Fujioka T. Demura S. Takatsuto S. Yoshida H. Fukuda (2001) ArticleTitleBrassinosteroid levels increase drastically prior to morphogenesis of tracheary elements Plant Physiol. 125 556–563

T. Yokota (1997) ArticleTitleThe structure, biosynthesis and function of brassinosteroids Trend. Plant. Sci. 2 137–143

P. Zimmermann M. Hirsch-Hoffmann L. Hennig W. Gruissem (2004) ArticleTitleGENEVESTIGATOR: Arabidopsis microarray database and analysis toolbox Plant Physiol. 136 2621–2632

Thông tin
Thông tin xuất bản

Plant Molecular Biology

Tập 57

129-140

Thông tin tác giả