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Khám Phá Chương Trình Biểu Thị Nectary Của Arabidopsis thaliana: Điều Tra Sự Biểu Hiện Gene Khác Nhau Trong Tissues Có Hoa Tiết Nectar
Tóm tắt
Nhiều loài thực vật có hoa thu hút thụ phấn bằng cách cung cấp phần thưởng là mật hoa. Đáng chú ý, các sự kiện phân tử liên quan đến sự phát triển của các cơ quan tiết nectar, cũng như quá trình tổng hợp và bài tiết nectar, vẫn còn chưa được hiểu rõ. Thực tế, cho đến nay, chưa có gen nào được chứng minh là có ảnh hưởng trực tiếp đến việc sản xuất de novo hoặc chất lượng của nectar hoa. Để giải quyết khoảng trống về kiến thức này, mảng ATH1 Affymetrix® GeneChip đã được sử dụng để điều tra hệ thống chương trình biểu thị của nectary Arabidopsis nhằm xác định các gen và con đường có thể liên quan đến việc sản xuất nectar. Trong nghiên cứu này, chúng tôi đã xác định một số lượng lớn các gen được biểu hiện khác nhau giữa các loại nectary bên tiết và các mô nectary giữa không tiết, cũng như giữa các nectary bên trưởng thành (sau phát hoa) và các nectary bên chưa trưởng thành (trước phát hoa). Biểu hiện trong các nectary cũng được so sánh với mười ba mô tham chiếu không phải nectary, từ đó 270 gen được xác định là có biểu hiện tăng cường đáng kể trong các nectary. Các mô hình biểu hiện của 14 gen giàu nectary cũng được xác nhận thông qua RT PCR. Khi xem xét các nhóm chức năng của các gen được tăng cường, các con đường liên quan đến việc điều chỉnh gen, chuyển hóa carbohydrate và chuyển hóa lipid đặc biệt phong phú trong các nectary so với các mô tham chiếu. Một số lượng lớn các gen được biểu hiện ưu tiên trong các nectary, cũng như giữa các loại nectary và các giai đoạn phát triển, đã được xác định. Một số giả thuyết liên quan đến các cơ chế sản xuất nectar và quy định việc này được đề xuất, và cung cấp một điểm khởi đầu cho các phương pháp di truyền đảo ngược nhằm xác định các cơ chế phân tử cơ bản của quá trình tổng hợp và bài tiết nectar.
Từ khóa
#nectar #Arabidopsis thaliana #gene expression #nectar production #transcriptomeTài liệu tham khảo
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