Tyrosine‐rich acidic matrix protein (TRAMP) is a tyrosine‐sulphated and widely distributed protein of the extracellular matrix

FEBS Letters - Tập 351 - Trang 433-436 - 1994
Euan G. Forbes1, Andrew D. Cronshaw1, Jonathan R.E. MacBeath1, David J.S. Hulmes1
1Department of Biochemistry, University of Edinburgh, Hugh Robson Buiding, George Square, Edinburgh, EH8 9XD, UK

Tóm tắt

Tyrosine‐rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co‐purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell—matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine TRAMP, which cross‐reacts with both the human and murine forms of the protein, we show by immunoblotting that TRAMP has a widespread tissue distribution, including skin, skeletal muscle, heart, lung, kidney, cartilage and bone. In cultures of human skin fibroblasts, TRAMP incorporates both [35S]sulphate and [3H]tyrosine and is secreted into the medium, as shown by immunoprecipitation. Amino acid analysis of immunoprecipitated TRAMP demonstrates that many of the tyrosine residues in TRAMP are sulphated.


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FEBS Letters

Tập 351

433-436

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