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Quy trình sản xuất hai bước dầu giàu axit linoleic liên hợp và diacylglycerol
Tóm tắt
Một quy trình hai bước được sử dụng để sản xuất lipid cấu trúc giàu diacylglycerol chứa chủ yếu các đồng phân c9,t11 và t10,c12 của axit linoleic liên hợp (CLA). Đầu tiên, một triacylglycerol (TAG) cấu trúc được tổng hợp bằng phản ứng axidolysis xúc tác bởi lipase giữa dầu ngô và CLA. Triacylglycerol cấu trúc này chứa 30.4 mol% CLA với 45.5% CLA chủ yếu nằm ở vị trí sn-1,3 của mạch glycerol. Sau đó, quá trình glycerolysis xúc tác bởi lipase được tiến hành giữa triacylglycerol cấu trúc và glycerol để sản xuất lipid cấu trúc giàu diacylglycerol. Sản phẩm cuối cùng chứa 6.8% monoacylglycerol, 31.5% diacylglycerol và 61.1% TAG sau 48 giờ phản ứng. Các thuộc tính hóa học đã chọn (thành phần axit béo, hàm lượng mono-, di- và triacylglycerol trong sản phẩm phản ứng) và tính chất vật lý (đặc điểm nóng chảy) đã được xác định bằng sắc ký lỏng hiệu năng cao (HPLC), sắc ký khí (GC) và nhiệt lượng kế quét vi sai (DSC).
Từ khóa
#axit linoleic liên hợp #diacylglycerol #triacylglycerol #lipase #glycerolysis #axidolysisTài liệu tham khảo
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