Tumor-Associated Macrophages as Major Players in the Tumor Microenvironment

Cancers - Tập 6 Số 3 - Trang 1670-1690
Theerawut Chanmee1, Pawared Ontong2, Kenjiro Konno3, Naoki Itano4,2,1
1Institute of Advanced Technology, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan
2Division of Engineering (Biotechnology), Graduate School of Engineering, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan
3Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan
4Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Kita-ku, Kyoto 603-8555, Japan

Tóm tắt

During tumor progression, circulating monocytes and macrophages are actively recruited into tumors where they alter the tumor microenvironment to accelerate tumor progression. Macrophages shift their functional phenotypes in response to various microenvironmental signals generated from tumor and stromal cells. Based on their function, macrophages are divided broadly into two categories: classical M1 and alternative M2 macrophages. The M1 macrophage is involved in the inflammatory response, pathogen clearance, and antitumor immunity. In contrast, the M2 macrophage influences an anti-inflammatory response, wound healing, and pro-tumorigenic properties. Tumor-associated macrophages (TAMs) closely resemble the M2-polarized macrophages and are critical modulators of the tumor microenvironment. Clinicopathological studies have suggested that TAM accumulation in tumors correlates with a poor clinical outcome. Consistent with that evidence, experimental and animal studies have supported the notion that TAMs can provide a favorable microenvironment to promote tumor development and progression. In this review article, we present an overview of mechanisms responsible for TAM recruitment and highlight the roles of TAMs in the regulation of tumor angiogenesis, invasion, metastasis, immunosuppression, and chemotherapeutic resistance. Finally, we discuss TAM-targeting therapy as a promising novel strategy for an indirect cancer therapy.

Từ khóa


Tài liệu tham khảo

Gordon, 2010, Alternative activation of macrophages: Mechanism and functions, Immunity, 32, 593, 10.1016/j.immuni.2010.05.007

Davies, 2013, Tissue-resident macrophages, Nat. Immunol., 14, 986, 10.1038/ni.2705

Murray, 2011, Protective and pathogenic functions of macrophage subsets, Nat. Rev. Immunol., 11, 723, 10.1038/nri3073

Mantovani, 2002, Macrophage polarization: Tumor-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes, Trends Immunol., 23, 549, 10.1016/S1471-4906(02)02302-5

Sica, 2012, Macrophage plasticity and polarization: In vivo veritas, J. Clin. Invest., 122, 787, 10.1172/JCI59643

Hao, 2012, Macrophages in tumor microenvironments and the progression of tumors, Clin. Dev. Immunol., 2012, 948098, 10.1155/2012/948098

Pollard, 2004, Tumour-educated macrophages promote tumour progression and metastasis, Nat. Rev. Cancer, 4, 71, 10.1038/nrc1256

Mantovani, 2010, Macrophages, innate immunity and cancer: Balance, tolerance, and diversity, Curr. Opin. Immunol., 22, 231, 10.1016/j.coi.2010.01.009

Movahedi, 2006, Classical and alternative activation of mononuclear phagocytes: Picking the best of both worlds for tumor promotion, Immunobiology, 211, 487, 10.1016/j.imbio.2006.06.002

Coffelt, 2010, Angiopoietin-2 regulates gene expression in TIE2-expressing monocytes and augments their inherent proangiogenic functions, Cancer Res., 70, 5270, 10.1158/0008-5472.CAN-10-0012

Movahedi, 2010, Different tumor microenvironments contain functionally distinct subsets of macrophages derived from Ly6C(high) monocytes, Cancer Res., 70, 5728, 10.1158/0008-5472.CAN-09-4672

Mantovani, 2008, Cancer-related inflammation, Nature, 454, 436, 10.1038/nature07205

Liao, 2014, LPLUNC1 suppresses IL-6-induced nasopharyngeal carcinoma cell proliferation via inhibiting the Stat3 activation, Oncogene, 33, 2098, 10.1038/onc.2013.161

Zijlmans, 2006, The absence of CCL2 expression in cervical carcinoma is associated with increased survival and loss of heterozygosity at 17q11.2, J. Pathol., 208, 507, 10.1002/path.1918

Tsutsui, 2005, Macrophage infiltration and its prognostic implications in breast cancer: The relationship with VEGF expression and microvessel density, Oncol. Rep., 14, 425

Gordon, 2005, Monocyte and macrophage heterogeneity, Nat. Rev. Immunol., 5, 953, 10.1038/nri1733

Dalton, 2014, Monocyte subpopulations in angiogenesis, Cancer Res., 74, 1287, 10.1158/0008-5472.CAN-13-2825

Roca, 2009, CCL2 and interleukin-6 promote survival of human CD11b+ peripheral blood mononuclear cells and induce M2-type macrophage polarization, J. Biol. Chem., 284, 34342, 10.1074/jbc.M109.042671

Zhang, 2010, Multiple roles of chemokine (C-C motif) ligand 2 in promoting prostate cancer growth, J. Natl. Cancer Inst., 102, 522, 10.1093/jnci/djq044

Qian, 2011, CCL2 recruits inflammatory monocytes to facilitate breast-tumour metastasis, Nature, 475, 222, 10.1038/nature10138

Mizutani, 2009, The chemokine CCL2 increases prostate tumor growth and bone metastasis through macrophage and osteoclast recruitment, Neoplasia, 11, 1235, 10.1593/neo.09988

Negus, 1995, The detection and localization of monocyte chemoattractant protein-1 (MCP-1) in human ovarian cancer, J. Clin. Invest., 95, 2391, 10.1172/JCI117933

Arenberg, 2000, Macrophage infiltration in human non-small-cell lung cancer: The role of CC chemokines, Cancer Immunol. Immunother., 49, 63, 10.1007/s002620050603

Nieto, 2014, CCL2 shapes macrophage polarization by GM-CSF and M-CSF: Identification of CCL2/CCR2-dependent gene expression profile, J. Immunol., 192, 3858, 10.4049/jimmunol.1302821

Gu, 2000, Control of TH2 polarization by the chemokine monocyte chemoattractant protein-1, Nature, 404, 407, 10.1038/35006097

Wu, 2008, CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process, J. Immunol., 181, 6384, 10.4049/jimmunol.181.9.6384

Milliken, 2002, Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites, Clin. Cancer Res., 8, 1108

Lin, 2001, Colony-stimulating factor 1 promotes progression of mammary tumors to malignancy, J. Exp. Med., 193, 727, 10.1084/jem.193.6.727

Kao, 1994, Characterization of a novel tumor-derived cytokine. Endothelial-monocyte activating polypeptide II, J. Biol. Chem., 269, 25106, 10.1016/S0021-9258(17)31505-3

Scholl, 1994, Anti-colony-stimulating factor-1 antibody staining in primary breast adenocarcinomas correlates with marked inflammatory cell infiltrates and prognosis, J. Natl. Cancer Inst., 86, 120, 10.1093/jnci/86.2.120

Dorsch, 1993, Macrophage colony-stimulating factor gene transfer into tumor cells induces macrophage infiltration but not tumor suppression, Eur. J. Immunol., 23, 186, 10.1002/eji.1830230129

Balkwill, 2004, Cancer and the chemokine network, Nat. Rev. Cancer, 4, 540, 10.1038/nrc1388

Murdoch, 2004, Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues, Blood, 104, 2224, 10.1182/blood-2004-03-1109

Solinas, 2009, Tumor-associated macrophages (TAM) as major players of the cancer-related inflammation, J. Leukoc. Biol., 86, 1065, 10.1189/jlb.0609385

Kobayashi, 2010, Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization, Cancer Res., 70, 7073, 10.1158/0008-5472.CAN-09-4687

Hascall, 1999, Mononuclear leukocytes preferentially bind via CD44 to hyaluronan on human intestinal mucosal smooth muscle cells after virus infection or treatment with poly(I·C), J. Biol. Chem., 274, 30747, 10.1074/jbc.274.43.30747

Day, 2002, Hyaluronan-binding proteins: Tying up the giant, J. Biol. Chem., 277, 4585, 10.1074/jbc.R100036200

Hascall, 2003, Mononuclear leukocytes bind to specific hyaluronan structures on colon mucosal smooth muscle cells treated with polyinosinic acid:polycytidylic acid: Inter-alpha-trypsin inhibitor is crucial to structure and function, Am. J. Pathol., 163, 121, 10.1016/S0002-9440(10)63636-X

Chiodoni, 2010, Matricellular proteins: From homeostasis to inflammation, cancer, and metastasis, Cancer Metastasis Rev., 29, 295, 10.1007/s10555-010-9221-8

Houghton, 2006, Elastin fragments drive disease progression in a murine model of emphysema, J. Clin. Invest., 116, 753, 10.1172/JCI25617

Solinas, 2010, Tumor-conditioned macrophages secrete migration-stimulating factor: A new marker for M2-polarization, influencing tumor cell motility, J. Immunol., 185, 642, 10.4049/jimmunol.1000413

Schaefer, 2005, The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages, J. Clin. Invest., 115, 2223, 10.1172/JCI23755

Midwood, 2009, Tenascin-C is an endogenous activator of Toll-like receptor 4 that is essential for maintaining inflammation in arthritic joint disease, Nat. Med., 15, 774, 10.1038/nm.1987

Kim, 2009, Carcinoma-produced factors activate myeloid cells through TLR2 to stimulate metastasis, Nature, 457, 102, 10.1038/nature07623

Scheibner, 2006, Hyaluronan fragments act as an endogenous danger signal by engaging TLR2, J. Immunol., 177, 1272, 10.4049/jimmunol.177.2.1272

Kuang, 2007, Tumor-derived hyaluronan induces formation of immunosuppressive macrophages through transient early activation of monocytes, Blood, 110, 587, 10.1182/blood-2007-01-068031

Lewis, 2000, Expression of vascular endothelial growth factor by macrophages is up-regulated in poorly vascularized areas of breast carcinomas, J. Pathol., 192, 150, 10.1002/1096-9896(2000)9999:9999<::AID-PATH687>3.0.CO;2-G

Grimshaw, 2002, Endothelin-2 is a macrophage chemoattractant: Implications for macrophage distribution in tumors, Eur. J. Immunol., 32, 2393, 10.1002/1521-4141(200209)32:9<2393::AID-IMMU2393>3.0.CO;2-4

Matschurat, 2003, Regulation of EMAP II by hypoxia, Am. J. Pathol., 162, 93, 10.1016/S0002-9440(10)63801-1

Korsisaari, 2007, Inhibition of VEGF-A prevents the angiogenic switch and results in increased survival of Apc+/min mice, Proc. Natl. Acad. Sci. USA, 104, 10625, 10.1073/pnas.0704213104

Casazza, 2013, Impeding macrophage entry into hypoxic tumor areas by Sema3A/Nrp1 signaling blockade inhibits angiogenesis and restores antitumor immunity, Cancer Cell, 24, 695, 10.1016/j.ccr.2013.11.007

Burke, 2002, Expression of HIF-1alpha by human macrophages: Implications for the use of macrophages in hypoxia-regulated cancer gene therapy, J. Pathol., 196, 204, 10.1002/path.1029

Talks, 2000, The expression and distribution of the hypoxia-inducible factors HIF-1alpha and HIF-2alpha in normal human tissues, cancers, and tumor-associated macrophages, Am. J. Pathol., 157, 411, 10.1016/S0002-9440(10)64554-3

Schioppa, 2003, Regulation of the chemokine receptor CXCR4 by hypoxia, J. Exp. Med., 198, 1391, 10.1084/jem.20030267

Ceradini, 2004, Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1, Nat. Med., 10, 858, 10.1038/nm1075

Eubank, 2011, Opposing roles for HIF-1alpha and HIF-2alpha in the regulation of angiogenesis by mononuclear phagocytes, Blood, 117, 323, 10.1182/blood-2010-01-261792

Grimshaw, 2001, Inhibition of monocyte and macrophage chemotaxis by hypoxia and inflammation—a potential mechanism, Eur. J. Immunol., 31, 480, 10.1002/1521-4141(200102)31:2<480::AID-IMMU480>3.0.CO;2-L

Sica, 2000, Defective expression of the monocyte chemotactic protein-1 receptor CCR2 in macrophages associated with human ovarian carcinoma, J. Immunol., 164, 733, 10.4049/jimmunol.164.2.733

Qian, 2010, Macrophage diversity enhances tumor progression and metastasis, Cell, 141, 39, 10.1016/j.cell.2010.03.014

Zaynagetdinov, 2011, A critical role for macrophages in promotion of urethane-induced lung carcinogenesis, J. Immunol., 187, 5703, 10.4049/jimmunol.1100558

Gordon, 2011, Diversity and plasticity of mononuclear phagocytes, Eur. J. Immunol., 41, 2470, 10.1002/eji.201141988

Sica, 2008, Macrophage polarization in tumour progression, Semin. Cancer Biol., 18, 349, 10.1016/j.semcancer.2008.03.004

Murdoch, 2008, The role of myeloid cells in the promotion of tumour angiogenesis, Nat. Rev. Cancer, 8, 618, 10.1038/nrc2444

Nakao, 2005, Infiltration of COX-2-expressing macrophages is a prerequisite for IL-1 beta-induced neovascularization and tumor growth, J. Clin. Invest., 115, 2979, 10.1172/JCI23298

Etoh, 2000, Angiogenin expression in human colorectal cancer: The role of focal macrophage infiltration, Clin. Cancer Res., 6, 3545

Lin, 2007, Vascular endothelial growth factor restores delayed tumor progression in tumors depleted of macrophages, Mol. Oncol., 1, 288, 10.1016/j.molonc.2007.10.003

Chen, 2011, Tumor-associated macrophages promote angiogenesis and melanoma growth via adrenomedullin in a paracrine and autocrine manner, Clin. Cancer Res., 17, 7230, 10.1158/1078-0432.CCR-11-1354

Laoui, 2014, Tumor hypoxia does not drive differentiation of tumor-associated macrophages but rather fine-tunes the M2-like macrophage population, Cancer Res., 74, 24, 10.1158/0008-5472.CAN-13-1196

Bingle, 2006, Macrophages promote angiogenesis in human breast tumour spheroids in vivo, Br. J. Cancer, 94, 101, 10.1038/sj.bjc.6602901

Guruvayoorappan, 2008, Tumor versus tumor-associated macrophages: How hot is the link?, Integr. Cancer Ther., 7, 90, 10.1177/1534735408319060

Murdoch, 2005, Macrophage migration and gene expression in response to tumor hypoxia, Int. J. Cancer, 117, 701, 10.1002/ijc.21422

Burke, 2003, Hypoxia-induced gene expression in human macrophages: Implications for ischemic tissues and hypoxia-regulated gene therapy, Am. J. Pathol., 163, 1233, 10.1016/S0002-9440(10)63483-9

Du, 2008, HIF1alpha induces the recruitment of bone marrow-derived vascular modulatory cells to regulate tumor angiogenesis and invasion, Cancer Cell, 13, 206, 10.1016/j.ccr.2008.01.034

Murdoch, 2007, Expression of Tie-2 by human monocytes and their responses to angiopoietin-2, J. Immunol., 178, 7405, 10.4049/jimmunol.178.11.7405

Venneri, 2005, Tie2 identifies a hematopoietic lineage of proangiogenic monocytes required for tumor vessel formation and a mesenchymal population of pericyte progenitors, Cancer Cell, 8, 211, 10.1016/j.ccr.2005.08.002

Pucci, 2009, A distinguishing gene signature shared by tumor-infiltrating Tie2-expressing monocytes, blood “resident” monocytes, and embryonic macrophages suggests common functions and developmental relationships, Blood, 114, 901, 10.1182/blood-2009-01-200931

Lewis, 2006, Distinct role of macrophages in different tumor microenvironments, Cancer Res., 66, 605, 10.1158/0008-5472.CAN-05-4005

Sangaletti, 2008, Macrophage-derived SPARC bridges tumor cell-extracellular matrix interactions toward metastasis, Cancer Res., 68, 9050, 10.1158/0008-5472.CAN-08-1327

Wyckoff, 2007, Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors, Cancer Res., 67, 2649, 10.1158/0008-5472.CAN-06-1823

Wyckoff, 2004, A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors, Cancer Res., 64, 7022, 10.1158/0008-5472.CAN-04-1449

Gocheva, 2010, IL-4 induces cathepsin protease activity in tumor-associated macrophages to promote cancer growth and invasion, Genes Dev., 24, 241, 10.1101/gad.1874010

Almholt, 2005, Reduced metastasis of transgenic mammary cancer in urokinase-deficient mice, Int. J. Cancer, 113, 525, 10.1002/ijc.20631

Ferjancic, 2012, Recruitment of monocytes/macrophages by tissue factor-mediated coagulation is essential for metastatic cell survival and premetastatic niche establishment in mice, Blood, 119, 3164, 10.1182/blood-2011-08-376426

Erler, 2009, Hypoxia-induced lysyl oxidase is a critical mediator of bone marrow cell recruitment to form the premetastatic niche, Cancer Cell, 15, 35, 10.1016/j.ccr.2008.11.012

Hiratsuka, 2006, Tumour-mediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis, Nat. Cell Biol., 8, 1369, 10.1038/ncb1507

Hiratsuka, 2008, The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase, Nat. Cell Biol., 10, 1349, 10.1038/ncb1794

Hiratsuka, 2013, Primary tumours modulate innate immune signalling to create pre-metastatic vascular hyperpermeability foci, Nat. Commun., 4, 1853, 10.1038/ncomms2856

Hiratsuka, 2011, Endothelial focal adhesion kinase mediates cancer cell homing to discrete regions of the lungs via E-selectin up-regulation, Proc. Natl. Acad. Sci. USA, 108, 3725, 10.1073/pnas.1100446108

Kryczek, 2006, B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma, J. Exp. Med., 203, 871, 10.1084/jem.20050930

Kuang, 2009, Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1, J. Exp. Med., 206, 1327, 10.1084/jem.20082173

Bates, 2006, Quantification of regulatory T cells enables the identification of high-risk breast cancer patients and those at risk of late relapse, J. Clin. Oncol., 24, 5373, 10.1200/JCO.2006.05.9584

Gobert, 2009, Regulatory T cells recruited through CCL22/CCR4 are selectively activated in lymphoid infiltrates surrounding primary breast tumors and lead to an adverse clinical outcome, Cancer Res., 69, 2000, 10.1158/0008-5472.CAN-08-2360

Curiel, 2004, Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival, Nat. Med., 10, 942, 10.1038/nm1093

Ishida, 2006, Specific recruitment of CC chemokine receptor 4-positive regulatory T cells in Hodgkin lymphoma fosters immune privilege, Cancer Res., 66, 5716, 10.1158/0008-5472.CAN-06-0261

Iellem, 2001, Unique chemotactic response profile and specific expression of chemokine receptors CCR4 and CCR8 by CD4(+)CD25(+) regulatory T cells, J. Exp. Med., 194, 847, 10.1084/jem.194.6.847

Mizukami, 2008, CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer, Int. J. Cancer, 122, 2286, 10.1002/ijc.23392

Clarke, 2004, Neurobiology: At the root of brain cancer, Nature, 432, 281, 10.1038/432281a

Dick, 2008, Stem cell concepts renew cancer research, Blood, 112, 4793, 10.1182/blood-2008-08-077941

Yi, 2011, Glioma-initiating cells: A predominant role in microglia/macrophages tropism to glioma, J. Neuroimmunol., 232, 75, 10.1016/j.jneuroim.2010.10.011

Yang, 2013, Tumor-associated macrophages regulate murine breast cancer stem cells through a novel paracrine EGFR/Stat3/Sox-2 signaling pathway, Stem Cells, 31, 248, 10.1002/stem.1281

Okuda, 2012, Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells, Cancer Res., 72, 537, 10.1158/0008-5472.CAN-11-1678

Fischer, 2007, Anti-PlGF inhibits growth of VEGF(R)-inhibitor-resistant tumors without affecting healthy vessels, Cell, 131, 463, 10.1016/j.cell.2007.08.038

Zhang, 2010, Depletion of tumor-associated macrophages enhances the effect of sorafenib in metastatic liver cancer models by antimetastatic and antiangiogenic effects, Clin. Cancer Res., 16, 3420, 10.1158/1078-0432.CCR-09-2904

Gazzaniga, 2007, Targeting tumor-associated macrophages and inhibition of MCP-1 reduce angiogenesis and tumor growth in a human melanoma xenograft, J. Invest. Dermatol., 127, 2031, 10.1038/sj.jid.5700827

Dineen, 2008, Vascular endothelial growth factor receptor 2 mediates macrophage infiltration into orthotopic pancreatic tumors in mice, Cancer Res., 68, 4340, 10.1158/0008-5472.CAN-07-6705

Ries, 2014, Targeting tumor-associated macrophages with Anti-CSF-1R antibody reveals a strategy for cancer therapy, Cancer Cell, 25, 846, 10.1016/j.ccr.2014.05.016

Mok, 2014, Inhibition of CSF-1 receptor improves the antitumor efficacy of adoptive cell transfer immunotherapy, Cancer Res., 74, 153, 10.1158/0008-5472.CAN-13-1816

Pyonteck, 2012, Deficiency of the macrophage growth factor CSF-1 disrupts pancreatic neuroendocrine tumor development, Oncogene, 31, 1459, 10.1038/onc.2011.337

Welford, 2011, TIE2-expressing macrophages limit the therapeutic efficacy of the vascular-disrupting agent combretastatin A4 phosphate in mice, J. Clin. Invest., 121, 1969, 10.1172/JCI44562

Tang, 2013, Anti-tumour strategies aiming to target tumour-associated macrophages, Immunology, 138, 93, 10.1111/imm.12023

Shime, 2012, Toll-like receptor 3 signaling converts tumor-supporting myeloid cells to tumoricidal effectors, Proc. Natl. Acad. Sci. USA, 109, 2066, 10.1073/pnas.1113099109

Coscia, 2010, Zoledronic acid repolarizes tumour-associated macrophages and inhibits mammary carcinogenesis by targeting the mevalonate pathway, J. Cell Mol. Med., 14, 2803, 10.1111/j.1582-4934.2009.00926.x

Zhang, 2013, Hydrazinocurcumin encapsuled nanoparticles “re-educate” tumor-associated macrophages and exhibit anti-tumor effects on breast cancer following STAT3 suppression, PLoS One, 8, e65896, 10.1371/journal.pone.0065896

Rolny, 2011, HRG inhibits tumor growth and metastasis by inducing macrophage polarization and vessel normalization through downregulation of PlGF, Cancer Cell, 19, 31, 10.1016/j.ccr.2010.11.009

Germano, 2013, Role of macrophage targeting in the antitumor activity of trabectedin, Cancer Cell, 23, 249, 10.1016/j.ccr.2013.01.008

Cieslewicz, 2013, Targeted delivery of proapoptotic peptides to tumor-associated macrophages improves survival, Proc. Natl. Acad. Sci. USA, 110, 15919, 10.1073/pnas.1312197110