Triticum mosaic virus: A New Virus Isolated from Wheat in Kansas

Plant Disease - Tập 92 Số 5 - Trang 808-817 - 2008
Dallas L. Seifers1, T. J. Martin1, T. L. Harvey2, John P. Fellers3, James P. Stack4, Marietta Ryba-White4, Stephen Haber5, Oleg V. Krokhin6, Victor Spicer6, Nicole E.J. Lovat6, Andrej Yamchuk6, Kenneth G. Standing6
1Professors, Kansas State University, Agricultural Research Center-Hays, Hays 67601-9228
2Professor, Department of Entomology, Kansas State University, Manhattan 66506.
3United States Department of Agriculture–Agricultural Research Service–PSERU, Department of Plant Pathology, Manhattan, KS 66506
4Department of Plant Pathology, Kansas State University, Manhattan,
5Cereal Research Centre, Agriculture & Agri-Food Canada, Winnipeg, Manitoba, Canada
6Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada

Tóm tắt

In 2006, a mechanically-transmissible and previously uncharacterized virus was isolated in Kansas from wheat plants with mosaic symptoms. The physiochemical properties of the virus were examined by purification on cesium chloride density gradients, electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), sequencing of the nucleotides and amino acids of the coat protein, and immunological reactivity. Purified preparations contained flexuous, rod-shaped particles that resembled potyviruses. The coat protein was estimated from SDS-PAGE to have a mass of approximately 35 kDa. Its amino acid sequence, as deduced from DNA sequencing of cloned, reverse-transcribed viral RNA and separately determined by time-of-flight mass spectrometry, was most closely related (49% similarity) to Sugarcane streak mosaic virus, a member of the Tritimovirus genus of the family Potyviridae. The virus gave strong positive reactions during enzyme-linked immunosorbent assays using polyclonal antibodies raised against purified preparations of the cognate virus but gave consistent negative reactions against antibodies to Wheat streak mosaic virus (WSMV), other wheat potyviruses, and the High Plains virus. When the virus was inoculated on the WSMV-resistant wheat cv. RonL, systemic symptoms appeared and plant growth was diminished significantly in contrast with WSMV-inoculated RonL. Taken together, the data support consideration of this virus as a new potyvirus, and the name Triticum mosaic virus (TriMV) is proposed.

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Tài liệu tham khảo

10.1007/s00705-006-0776-1

Berger, P. H., Barnett, O. W., Brunt, A. A., Colinet, D., Edwardson, J. R., Hammond, J. Hill, J. H., Jordan, R. L., Kashiwazaki, S., Makkouk, K., Morales, F. J., Rybicki, E., Spence, N., Ohki, S. T., Uyeda, I., van Zaayen, A., and Vetten, H. J. 2000. Family Potyviridae. Pages 703-724 in: Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses. M. H. V van Regenmortel, C. M. Fauquet, D. H. L Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, C. R. Pringle, and R. B. Wickner, eds. Academic Press, New York.

Brakke, M. K. 1971. Soil-borne wheat mosaic virus. In: Descriptions of Plant Viruses, No. 77. CMI/AAB, Kew, Surrey, UK.

10.1094/Phyto-80-1401

Brandes J., 1957, Nachrbl. Deut. Pflanzenschutzdientz (Braunschweig), 9, 151

10.1016/0042-6822(59)90022-4

Bollig, D. M., and Edelstein, S. J. 1991. Immunobloting. Pages 181-208 in: Protein Methods. Wiley-Liss, New York.

10.1099/0022-1317-34-3-475

10.2135/cropsci2002.1381

10.1006/mpev.1998.0535

10.1007/s007050050519

10.1007/s00705-003-0015-y

Hema M., 1997, Indian J. Virol., 13, 125

10.1007/s00705-002-0851-1

10.1094/PD-80-1387

Kinter, M., and Sherman, N. E. 2000. Protein Sequencing and Identification Using Tandem Mass Spectrometry. Wiley Interscience, New York.

10.1021/ac061017o

10.1074/mcp.M400031-MCP200

Krokhin, O. V., Spicer, V., Standing, K. G., Wilkins, J. A, and Ens, W. 2006. "SMART" (Search by Mass and Retention Time)-a new tool for HPLC-MALDI MS peptide mass fingerprinting. Poster 533, 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle.

10.1038/227680a0

10.1016/0003-2697(78)90792-3

10.1016/0076-6879(86)18109-2

Lengkeck, V. H. 1979. Wheat Virus Disease in Southwestern Kansas. Coop. Ext. Rep. Kansas State University, Manhattan.

10.1002/1097-0231(20000630)14:12<1047::AID-RCM990>3.0.CO;2-E

10.1016/j.jviromet.2006.03.023

10.1099/0022-1317-83-4-895

Rao G. P., 2004, Indian J. Biotechnol., 3, 542

Rochow, W. F. 1970. Barley yellow dwarf virus. In: Descriptions of Plant Viruses, No. 4. CMI/AAB, Kew, Surrey, UK.

10.1094/PD-76-0564

Seifers, D. L. 2006. Identification of variants of the High Plains virus in Kansas. (Abstr.) Phytopathology 96:S106.

10.1094/Phyto-78-208

10.1007/s00705-004-0411-y

10.1094/PD-77-0335

10.1094/PD-79-0853

10.1094/PDIS.1999.83.10.905

10.1094/PD-80-0179

10.1094/PDIS.1997.81.10.1161

10.1094/PD-89-0888

10.1094/Phyto-78-703

10.1094/PD-79-1104

10.1094/PDIS-91-8-1029

10.1094/PD-90-0623

10.1094/PHYTO.2000.90.5.505

10.1094/PDIS.2004.88.8.824

10.1074/jbc.M100189200

10.1074/jbc.M308506200

10.1002/(SICI)1097-0231(19970615)11:9<1015::AID-RCM958>3.0.CO;2-H

10.1021/ac0013709

10.2307/3625622

10.1094/PD-72-0832

10.1016/j.virol.2005.11.030

Slykhuis J. T., 1955, Phytopathology, 45, 116

Slykhuis, J. T. 1976. Wheat spindle streak mosaic virus. In: Descriptions of Plant Viruses, No. 167. CMI/AAB, Kew, Surrey, UK.

10.1016/j.sbi.2003.09.005

10.1073/pnas.76.9.4350

van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L, Carstens, E. B., Estes, M. K., Lemon, S. M., Maniloff, J., Mayo, M. A., Pringle, C. R., and Wickner, R. B. 2000. Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, New York.

Willis, W. G. 1981. The 1981 Wheat Streak Mosaic Epidemic in Kansas. Coop. Ext. Rep. Kansas State University, Manhattan.

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Plant Disease

Tập 92 Số 5

808-817

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