Treatment of growth plate injury with microencapsulated chondrocytes

Springer Science and Business Media LLC - Tập 18 - Trang 655-662 - 2013
Wen-Chao Li1, Rui-Jiang Xu1, Yi-Long Xue2, Jing-Xiang Huang3, Yu-Hong Gao2
1Department of Pediatric Orthopaedic Surgery, Chinese People’s Liberation Army General Hospital, Beijing, China
2Institute of Basic Medical Sciences, Chinese People’s Liberation Army General Hospital, Beijing, China
3Institute of Orthopedics, Chinese People’s Liberation Army General Hospital, Beijing, China

Tóm tắt

Tissue-engineered cartilage has provided a promising method in the treatment of physeal growth arrest. This study was designed to investigate transplantation of microencapsulated allogeneic chondrocytes to treat the injured growth plate. Allogeneic chondrocytes were encapsulated within alginate-polylysinealginate semipermeable membranes. Microencapsulated chondrocytes co-cultured with Bone Mesenchymal Stem Cells (BMSCs) were evaluated whether it could promote chondrogenesis of BMSCs. An experiment model of an injured growth plate was made by resecting the lateral half of the right distal femur physis in rabbits. Microencapsulated allogeneic chondrocytes, allogeneic chondrocytes as well as empty microcapsules were grafted into growth plate defects of 6-week-old rabbits. Histological and radiographic examinations were examined after transplantation up to 16 weeks. The histological study showed that BMSCs co-cultured with microencapsulated chondrocytes could produce GAG and II collagen similarly with chondrocytes. Angular deformity and length discrepancy in the group with microencapsulated allogeneic chondrocytes were less than those in other groups (p < 0.001). The histological study confirmed the viability of microencapsulated chondrocytes at 16 weeks postoperatively. The neogenetic chondrocytes of columnar arrangement have been found in the growth plate defect to prevent early ossification and closure of the growth plate. The histological study confirmed the viability of microencapsulated chondrocytes at 16 weeks postoperatively. The neogenetic chondrocytes of columnar arrangement have been found in the growth plate defect to prevent early ossification and closure of the growth plate.

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Tài liệu tham khảo

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Springer Science and Business Media LLC

Tập 18

655-662

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