Transplantation of cultured sympathetic ganglionic neurons into Parkinsonian rat brain: Survival and function of graft
Tóm tắt
The superior cervical ganglia (SCG) of newborn rats, which had been cultured as expiants for varying periods of time, were transplanted into the striatum of rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal dopamine pathway to examine the survival and functional properties of the sympathetic neurons maintained in long-term culture prior to grafting. In the rats given the SCG cultured in vitro for 2 weeks, apomorphine-induced rotational behaviour was satisfactory reduced. The rats receiving the SCG from 4-week-old cultures showed only modest behavioural changes. The grafting of the SCG cultured for 6 weeks in vitro did not affect the rotational behaviour. These behavioural data corresponded with the histological assessment of the graft survival by use of catecholamine histofluorescence. The present results suggest the critical time period in vitro which might allow the cultured sympathetic neurons to be successfully grafted.
Tài liệu tham khảo
Bandtlow CE, Heumann R, Schwab ME, Thoenen HJ (1987) Cellular localization of nerve growth factors synthesis by in situ hybridization. EMBO J 6: 891–899
Barbin G, Selak I, Manthorpe M, Varon S (1984) Use of central neuronal cultures for the detection of neuronotrophic agents. Neuroscience 12: 33–43
Brundin P, Barbin G, Strecker RE, Isacson O, Prochiantz A, Björklund A (1988) Survival and function of dissociated rat dopamine neurones grafted at different developmental stages or after being cultured in vitro. Dev Brain Res 39: 233–243
Collins F (1980) Neurite outgrowth induced by the substrate associated material from nonneuronal cells. Dev Biol 79: 247–252
de la Torre JC (1980) An improved approach to histofluorescence using the SPG method for tissue monoamines. J Neurosci Meth 3: 1–5
Doupe AJ, Patterson PH, Landis SC (1985) Environmental influences in the development of neural crest derivatives: glucocorticoids, growth factors, and chromaffin cell plasticity. J Neurosci 5: 2119–2142
Doupe AJ, Patterson PH, Landis SC (1985) Small intensely fluorescent cells in culture: Role of gluococorticoid and growth factors in their development and interconversions with neural crest derivatives. J Neurosci 5: 2143–2160
Dunnett SB, Björklund A, Stenevi U (1983) Dopamine-rich transplants in experimental parkinsonism. Trends Neurosci 6: 266–270
Elfvin LG, Hökfelt T, Goldstein M (1975) Fluorescence microscopical, immunohistochemical and ultrastructural studies on sympathetic ganglia of the guinea pig, with special reference to the SIF cells and their catecholamine content. J Ultrastruct Res 51: 377–396
Eränkö O, Eränkö L, Hill CE, Burnstock G (1972) Hydrocortisone-induced increase in the number of small intesely fluorescent cells and their histochemical demonstrable catecholamine content in cultures of sympathetic ganglia of the newborn rat. Histochem J 4: 49–58
Ezerman EB, Kromer LF (1987) Transplants of cholinergic septal explants reinnervate adult rodent hippocampus. Brain Res Bull 18: 337–344
Freed WJ, Karoum F, Spoor E, Morihisa JM, Olson L, Wyatt RJ (1983) Catecholamine content of intracerebral adrenal medulla graft. Brain Res 269: 184–189
Freed WJ, Morihisa JM, Spoor E, Hoffer BJ, Olson L, Seiger A, Wyatt RJ (1981) Transplanted adrenal chromaffin cells in rat brain reducelesion-induced rotational behavior. Nature 292: 351–352
Fukuda J, Yamaguchi K, Akimoto S, Tada Y (1985) NGF-dependent and -independent growth of neurites from sympathetic ganglion cells of the aged human in a serum-free culture. Neurosci Res 2: 460–471
Gerold N, Enz A, Scröder H (1982) Biochemical analysis of catecholamines in small intensely fluorescent (SIF) cell clusters of the rat superior cervical ganglion. J Neurosci Meth 6: 287–292
Gibbs RB, Pixley SKR, Cotoman CW (1986) Transplantation of septal neurons maintained in long-term culture. Brain Res 382: 409–415
Hökfelt T, Ungerstedt U (1973) Specificity of 6-hydroxydopamine induced degeneration of central monoamine neurones: an electron and fluorescence microscopic study with special reference to the intracerebral injection on the nigro-striatal dopamine system. Brain Res 60: 269–297
Itakura T (1983) Aminergic and cholinergic innervations of the spinal cord blood vessels of cats. A histochemical study. J Neurosurg 58: 900–905
Itakura T, Kamei I, Nakai K, Naka Y, Nakakita K, Imai H, Komai N (1989) Autotransplantation of the superior cervical ganglion into the brain. A possible therapy for Parkinson's disease. J Neurosurg 68: 955–959
Itakura T, Kasamatsu T, Pettigrew JD (1981) Norepinephrinecontaining terminals in kitten visual cortex: laminar distribution and ultrastructure. Neuroscience 6: 159–175
Itakura T, Komai N, Nakai M, Nakai K, Ooiwa Y, Ryujin Y (1991) Transplantation of autologous sympathetic ganglion in the brain with parkinsonism. Functional Neurosurgery 30: 157–164
Johnson MI, Ross CD, Bunge RP (1980) Morphological and biochemical studies on the development of cholinergic properties in cultured sympathetic neurons. Dependence on postnatal sympathetic neurons. J Cell Biol 84: 692–704
Kamo H, Kim SU, McGeer PL, Shin DH (1986) Functional recovery in a rat model of Parkinson's disease following transplantation of cultured human sympathetic neurons. Brain Res 397: 372–376
Lindsay RM, Raisman G (1984) An autoradiographic study of neuronal development, vascularization and glial cell migration from hippocampal transplants labeled in intermediate expiant culture. Neuroscience 12: 513–530
McLoon LK, McLoon SC, Lund RD (1985) Cultured embryonic retinae transplanted to rat brain: differentiation and formation of projections to host superior colliculus. Brain Res 226: 15–31
Nagatsu T, Kato T, Numata Y, Ikuta K, Sabo M, Nagatsu I, Kondo Y, Inagaki S, Iizuka R, Hori A, Narabayashi H (1977) Phenylethanolamine N-methyltransferase and other enzymes of catecholamine metabolism in human brain. Clin Chim Acta 75: 221–232
Nakai M, Itakura T, Kamei I, Nakai K, Naka Y, Imai H, Komai N (1990) Autologous transplantation of the superior cervical ganglion into the brain of parkinsonian monkeys. J Neurosurg 72: 91–95
Narabayashi H, Kondo T, Hayashi A, Suzuki T, Nagatsu T (1981) L-threo-3,4-dihydroxylserine treatment for akinesia and freezing of parkinsonism. Proc Jpn Acad 57: 351–354
Ohta H, Ishiyama J, Saito H, Nishiyama N (1991) Effects of pretreatment with basic fibroblast growth factor, epidermal growth factor and nerve growth factor on neuron survival and neovascularization of superior cervical ganglion transplanted into the third ventricle in rats. Japan J Pharmacol 55: 255–262
Ohta H, Nishizuka M, Arai Y, Saito H (1991) Effect of nerve grwoth factor (NGF) on survival of superior cervical ganglion (SCG) transplanted into the third ventricle in rats. Jp J Pharmacol 55: 255–262
Pelligriono LJ, Pelligrino AS, Cushman AJ (1979) A stereotaxic atlas of the rat brain, 2nd Ed. Plenum, New York
Perlow MJ, Freed WJ, Hoffer BJ, Seiger A, Olson L, Wyatt RJ (1979) Brain grafts reduce motor abnormalities produced by destruction of nigrostriatal dopamine system. Science 204: 643–647
Riederer P, Birkmayer W, Seemann D (1977) Brain noradrenalin and 3-methoxy-4-hydroxyphenylglycol in Parkinson's syndrome. J Neural Transm 41: 241–251
Rinne UK, Sonninen V (1973) Brain catecholamines and their metabolites in parkinsonian patients. Treatment with levodopa alone or combined with a decarboxylase inhibitor. Arch Neurol 28: 107–110
Rogers SL, Letourneau PC, Palm SL, McGarthy J, Furcht LT (1983) Neurite extension by peripheral and central nervous system neurons in response to substratum-bound fibronectin and laminin. Dev Biol 98: 212–220
Strecker RE, Miao R, Loring JF (1989) Survival and function aggregate cultures of rat fetal dopamine neurons grafted in a rat model of Parkinson's disease. Exp Brain Res 76: 315–322
Thoenen H, Barde Y-A (1980) Physiology of nerve growth factor. Physiol Rev 60: 1284–1335
Ungerstedt U, Arbuthnott GW (1970) Quantitative recording of rotational behavior in rats after 6-hydroxy-dopamine lesions of the nigrostriatal dopamine system. Brain Res 24: 485–493
Varon S, Skapper SD, Barbin G, Selack I, Manthorpe M (1984) Low molecular weight agents support survival of cultured neurons from the central nervous system. J Neurosci Res 4: 654–658
Varon S, Skaper SD, Manthorpe M (1981) Trophic activities for dorsal root and sympathetic ganglionic neurons in media conditioned by Schwann and other peripheral cells. Dev Brain Res 1: 73–87