Transglutaminase 2 activity promotes membrane resealing after mechanical damage in the lung cancer cell line A549

Cell Biology International - Tập 32 - Trang 928-934 - 2008
Yoshiki Kawai1, Fumitaka Wada1, Yoshiaki Sugimura1, Masatoshi Maki1, Kiyotaka Hitomi1
1Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan

Tóm tắt

AbstractTransglutaminase is a Ca2+‐dependent enzyme catalyzing protein cross‐linking reactions. We investigated the contribution of this enzyme to the resealing of the injured plasma membrane in animal cells, using a lung cancer‐derived cell line, A549. After mechanical injury by razor‐scratching, the level of membrane resealing was estimated by differential incorporation of dextrans labeled with two distinct fluorescent dyes. The recovery level was decreased in the presence of excess primary amine, as a competitive inhibitor of transglutaminase. We established a cell line that stably expresses shRNA (short hairpin RNA) to specifically inhibit the expression of TGase 2 (tissue‐type isozyme of transglutaminase) and confirmed the suppressed resealing level in the cell. Furthermore, additional expression of TGase 2 rescued the ability for membrane resealing. These results show that, after mechanical damage, this enzyme appeared to contribute to membrane resealing.

Tài liệu tham khảo

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Thông tin xuất bản

Cell Biology International

Tập 32

928-934

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