Giải trình tự transcriptome của mô thân rễ của Sinopodophyllum hexandrum ở hai nhiệt độ

Shaw JMH: New combinations for the varieties of Sinopodophyllum hexandrum. Hanburyana. 2009, 4: 33-39. Stähelin HF, von Wartburg A: The chemical and biological route from podophyllotoxin glucoside to etoposide: ninth Cain memorial Award lecture. Cancer Res. 1991, 51: 5-15. Nadeem M, Palni LMS, Purohit AN, Pandey H, Nandi SK: Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb. Biol Conserv. 2000, 92: 121-129. 10.1016/S0006-3207(99)00059-2. Giri A, Lakshmi Narasu M: Production of podophyllotoxin from Podophyllum hexandrum: a potential natural product for clinically useful anticancer drugs. Cytotechnology. 2000, 34: 17-26. 10.1023/A:1008138230896. You Y: Podophyllotoxin derivatives: current synthetic approaches for new anticancer agents. Curr Pharm Des. 2005, 11: 1695-1717. 10.2174/1381612053764724. Kim E, Donohue K: Local adaptation and plasticity of Erysimum capitatum to altitude: its implications for responses to climate change. J Ecol. 2013, 101: 796-805. 10.1111/1365-2745.12077. Kushwaha R, Pandey S, Chanda S, Bhattacharya A, Ahuja PS: Temperature dependent growth and emergence of functional leaves: an adaptive mechanism in the seedlings of the western Himalayan plant Podophyllum hexandrum. J Plant Res. 2008, 121: 299-309. 10.1007/s10265-008-0149-9. Singh A, Purohit AN: Light and temperature effects on physiological reactions on alpine and temperate populations of Podophyllum hexandrum Royle. J Herbs Spices Med Plants. 1997, 5: 57-66. Schrader J, Moyle R, Bhalerao R, Hertzberg M, Lundeberg J, Nilsson P, Bhalerao RP: Cambial meristem dormancy in trees involves extensive remodelling of the transcriptome. Plant J. 2004, 40: 173-187. 10.1111/j.1365-313X.2004.02199.x. Derory J, Léger P, Garcia V, Schaeffer J, Hauser MT, Salin F, Luschnig C, Plomion C, Glössl J, Kremer A: Transcriptome analysis of bud burst in sessile oak (Quercus petraea). New Phytol. 2006, 170: 723-738. 10.1111/j.1469-8137.2006.01721.x. Paul A, Kumar S: Responses to winter dormancy, temperature, and plant hormones share gene networks. Funct Integr Genomics. 2011, 11: 659-664. 10.1007/s10142-011-0233-4. Gahlan P, Singh HR, Shankar R, Sharma N, Kumari A, Chawla V, Ahuja PS, Kumar S: De novo sequencing and characterization of Picrorhiza kurrooa transcriptome at two temperatures showed major transcriptome adjustments. BMC Genomics. 2012, 13: 126-10.1186/1471-2164-13-126. Venkataraman K: India’s Biodiversity Act 2002 and its role in conservation. Trop Ecol. 2009, 50: 23-30. Bhattacharyya D, Sinha R, Ghanta S, Chakraborty A, Hazra S, Chattopadhyay S: Proteins differentially expressed in elicited cell suspension culture of Podophyllum hexandrum with enhanced podophyllotoxin content. Proteome Sci. 2012, 10: 34-10.1186/1477-5956-10-34. Sultan P, Shawl AS, Abdellah AA, Ramteke PW: Isolation, Characterization and Comparative Study on Podophyllotoxin and Related Glycosides of Podophyllum heaxandrum. Curr Res J Biol Sci. 2010, 2: 345-351. Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG: Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis. J Biol Chem. 2013, 288: 466-479. 10.1074/jbc.M112.400689. Compound extraction of Podophyllum peltatum. [http://uic.edu/pharmacy/MedPlTranscriptome/d_podo_p_3.html] Muoki RC, Paul A, Kumari A, Singh K, Kumar S: An improved protocol for the isolation of RNA from roots of tea (Camellia sinensis (L.) O. Kuntze). Mol Biotechnol. 2012, 52: 82-88. 10.1007/s12033-011-9476-5. GenScript real-time PCR primer design. [https://www.genscript.com/ssl-bin/app/primer] Pfaffl WP, Horgan GW, Dempfle L: Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002, 30: e36-10.1093/nar/30.9.e36. Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, Li S, Yang H, Wang J, Wang J: De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2009, 20: 265-272. Li W, Godzik A: Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences. Bioinformatics. 2006, 22: 1658-1659. 10.1093/bioinformatics/btl158. UniProt Consortium: Reorganizing the protein space at the Universal Protein Resource (UniProt). Nucleic Acids Res. 2011, 40 (Database issue): D71-D75. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G: Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000, 25: 25-29. 10.1038/75556. Ogata H, Goto S, Sato K, Fujibuchi W, Bono H, Kanehisa M: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Res. 1999, 27: 29-34. 10.1093/nar/27.1.29. Webb EC: International Union of Biochemistry and Molecular Biology. Nomenclature Committee. Enzyme nomenclature: Recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology on the nomenclature and classification of enzymes. 1992, San Diego: Academic Press, 862- Schmid R, Blaxter ML: annot8r: GO, EC and KEGG annotation of EST datasets. BMC Bioinformatics. 2008, 9: 180-10.1186/1471-2105-9-180. Pérez-Rodríguez P, Riaño-Pachón DM, Corrêa LG, Rensing SA, Kersten B, Mueller-Roeber B: PlnTFDB: updated content and new features of the plant transcription factor database. Nucleic Acids Res. 2010, 40 (Database issue): D822-D827. Ruepp A, Zollner A, Maier D, Albermann K, Hani J, Mokrejs M, Tetko I, Güldener U, Mannhaupt G, Münsterkötter M, Mewes HW: The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes. Nucleic Acids Res. 2004, 32: 5539-5545. 10.1093/nar/gkh894. Lamesch P, Berardini TZ, Li D, Swarbreck D, Wilks C, Sasidharan R, Muller R, Dreher K, Alexander DL, Garcia-Hernandez M, Karthikeyan AS, Lee CH, Nelson WD, Ploetz L, Singh S, Wensel A, Huala E: The Arabidopsis Information Resource (TAIR): improved gene annotation and new tools. Nucleic Acids Res. 2012, 40 (Database issue): D1202-D1210. Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Lu F, Marchler GH, Mullokandov M, Omelchenko MV, Robertson CL, Song JS, Thanki N, Yamashita RA, Zhang D, Zhang N, Zheng C, Bryant SH: CDD: a Conserved domain database for the functional annotation of proteins. Nucleic Acids Res. 2011, 39: 225-229. 10.1093/nar/gkq769. Trapnell C, Pachter L, Salzberg SL: TopHat: discovering splice junctions with RNA-seq. Bioinformatics. 2009, 25: 1105-1111. 10.1093/bioinformatics/btp120. Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ, Pachter L: Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010, 28: 511-515. 10.1038/nbt.1621. MISA Program. [http://pgrc.ipk-gatersleben.de/misa] Zhang P, Dreher K, Karthikeyan A, Chi A, Pujar A, Caspi R, Karp P, Kirkup V, Latendresse M, Lee C, Mueller LA, Muller R, Rhee SY: Creation of a genome-wide metabolic pathway database for Populus trichocarpa using a new approach for reconstruction and curation of metabolic pathways for plants. Plant Physiol. 2010, 153: 1479-1491. 10.1104/pp.110.157396. Salick J, Fangb Z, Byg A: Eastern Himalayan alpine plant ecology, Tibetan ethnobotany, and climate change. Global Environ Chang. 2009, 19: 147-155. 10.1016/j.gloenvcha.2009.01.008. Zobayed SM, Afreen F, Kozai T: Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’s wort. Plant Physiol Biochem. 2005, 43: 977-984. 10.1016/j.plaphy.2005.07.013. Alam MA, Gulati P, Aswini KG, Gyan PM, Pradeep KN: Assessment of genetic diversity among Podophyllum hexandrum genotypes of northwestern Himalayan region for podophyllotoxin production. Indian J Biotechnol. 2009, 8: 391-399. Chandra S: Effect of altitude on energy exchange characteristics of some alpine medicinal crops from central Himalayas. J Agron Crop Sci. 2004, 190: 13-20. 10.1046/j.0931-2250.2003.00064.x. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410. 10.1016/S0022-2836(05)80360-2. Vinogradov AE: DNA helix: the importance of being GC-rich. Nucleic Acids Res. 2003, 31: 1838-1844. 10.1093/nar/gkg296. Nestor CE, Monckton DG: Correlation of inter-locus polyglutamine toxicity with CAG•CTG triplet repeat expandability and flanking genomic DNA GC content. PLoS One. 2011, 6: e28260-10.1371/journal.pone.0028260. Holland JB, Helland SJ, Sharopova N, Rhyne DC: Polymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat. Genome. 2001, 44: 1065-1076. 10.1139/g01-110. Musashi M, Ota S, Shiroshita N: The role of protein kinase C isoforms in cell proliferation and apoptosis. Int J Hematol. 2000, 72: 12-19. Annadurai RS, Jayakumar V, Mugasimangalam RC, Katta MA, Anand S, Gopinathan S, Sarma SP, Fernandes SJ, Mullapudi N, Murugesan S, Rao SN: Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties. BMC Genomics. 2012, 13: 663-10.1186/1471-2164-13-663. Smith IK, Polle A, Rennenberg H: Glutathione. Stress Responses in Plants: Adaptation and Acclimation Mechanisms. Edited by: Alscher RG, Cumming JR. 1992, New York: Wiley Liss Inc, 201-215. Vu JCV, Allen LH, Gesch RW: Up-regulation of photosynthesis and sucrose metabolism enzymes in young expanding leaves of sugarcane under elevated growth CO2. Plant Sci. 2006, 171: 123-131. 10.1016/j.plantsci.2006.03.003. An D, Yang J, Zhang P: Transcriptome profiling of low temperature treated cassava apical shoots showed dynamic responses of tropical plant to cold stress. BMC Genomics. 2012, 13: 64-10.1186/1471-2164-13-64. Hotton SK, Callis J: Regulation of cullin RING ligases. Annu Rev Plant Biol. 2008, 59: 467-489. 10.1146/annurev.arplant.58.032806.104011. TAIR. [https://www.arabidopsis.org/servlets/TairObject?name=AT3G12670&type=locus] Henriksson E, Olsson AS, Johannesson H, Johansson H, Hanson J, Engström P, Söderman E: Homeodomain leucine zipper class I genes in Arabidopsis. Expression patterns and phylogenetic relationships. Plant Physiol. 2005, 139: 509-518. 10.1104/pp.105.063461. Ha CM, Jun JH, Nam HG, Fletcher JC: BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana. Plant Cell Physiol. 2004, 45: 1361-1370. 10.1093/pcp/pch201. Jofuku KD, den Boer BG, Van Montagu M, Okamuro JK: Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell. 1994, 6: 1211-1225. 10.1105/tpc.6.9.1211. Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF, Coupland G: Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science. 2000, 288: 1613-1616. 10.1126/science.288.5471.1613. Kim WC, Reca IB, Kim Y, Park S, Thomashow MF, Keegstra K, Han KH: Transcription factors that directly regulate the expression of CSLA9 encoding mannan synthase in Arabidopsis thaliana. Plant Mol Biol. 2014, 84: 577-587. 10.1007/s11103-013-0154-9. Apuya NR, Yadegari R, Fischer RL, Harada JJ, Zimmerman JL, Goldberg RB: The Arabidopsis embryo mutant schlepperless has a defect in the chaperonin-60alpha gene. Plant Physiol. 2001, 126: 717-730. 10.1104/pp.126.2.717. Davletova S, Schlauch K, Coutu J, Mittler R: The zinc-finger protein Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis. Plant Physiol. 2005, 139: 847-856. 10.1104/pp.105.068254. Polidoros AN, Mylona PV, Pasentsis K, Scandalios JG, Tsaftaris AS: The maize alternative oxidase 1a (Aox1a) gene is regulated by signals related to oxidative stress. Redox Rep. 2005, 10: 71-78. 10.1179/135100005X21688. Diédhiou CJ, Popova OV, Dietz KJ, Golldack D: The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice. BMC Plant Biol. 2008, 8: 49-10.1186/1471-2229-8-49. Wang W, Vinocur B, Shoseyov O, Altman A: Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends Plant Sci. 2004, 9: 244-252. 10.1016/j.tplants.2004.03.006. Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K: AP2/ERF family transcription factors in plant abiotic stress responses. Biochim Biophys Acta. 2012, 1819: 86-96. 10.1016/j.bbagrm.2011.08.004. Yi Y, Guerinot ML: Genetic evidence that induction of root Fe(III) chelate reductase activity is necessary for iron uptake under iron deficiency. Plant J. 1996, 10: 835-844. 10.1046/j.1365-313X.1996.10050835.x. Sun S, Yu JP, Chen F, Zhao TJ, Fang XH, Li YQ, Sui SF: TINY, a dehydration-responsive element (DRE)-binding protein-like transcription factor connecting the DRE- and ethylene-responsive element-mediated signaling pathways in Arabidopsis. J Biol Chem. 2008, 283: 6261-6271. 10.1074/jbc.M706800200. Wang J, Chen L, Huang S, Liu J, Ren X, Tian X, Qiao J, Zhang W: RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803. Biotechnol Biofuels. 2012, 5: 89-10.1186/1754-6834-5-89. Xu L, Zhu L, Tu L, Liu L, Yuan D, Jin L, Long L, Zhang X: Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry. J Exp Bot. 2011, 62: 5607-5621. 10.1093/jxb/err245. Heim MA, Jakoby M, Werber M, Martin C, Weisshaar B, Bailey PC: The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity. Mol Biol Evol. 2003, 20: 735-747. 10.1093/molbev/msg088. Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L: MYB transcription factors in Arabidopsis. Trends Plant Sci. 2010, 15: 573-581. 10.1016/j.tplants.2010.06.005. Bienz M: The PHD finger, a nuclear protein-interaction domain. Trends Biochem Sci. 2006, 31: 35-40. 10.1016/j.tibs.2005.11.001. Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K: Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell. 2004, 16: 2481-2498. 10.1105/tpc.104.022699. Englbrecht CC, Schoof H, Böhm S: Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome. BMC Genomics. 2004, 5: 39-10.1186/1471-2164-5-39. Jackson DE, Dewick PM: Biosynthesis of Podophyllum lignans-II. Interconversion of aryltetralin lignans in Podophyllum hexandrum. Phytochemistry. 1984, 23: 1037-1042. 10.1016/S0031-9422(00)82604-7. Hoffmann L, Maury S, Martz F, Geoffroy P, Legrand M: Purification, cloning and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism. J Biol Chem. 2003, 278: 95-103. Schoch G, Goepfert S, Morant M, Hehn A, Meyer D, Ullmann P, Werck-Reichhart D: CYP98A3 from Arabidopsis thaliana is a 3′-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway. J Biol Chem. 2001, 276: 36566-36574. 10.1074/jbc.M104047200. Fraser CM, Chapple C: The phenylpropanoid pathway in Arabidopsis. Arabidopsis Book. 2011, 9: e0152- Davin LB, Wang HB, Crowell AL, Bedgar DL, Martin DM, Sarkanen S, Lewis NG: Stereoselective bimolecular phenoxy radical coupling by an auxiliary (dirigent) protein without an active center. Science. 1997, 275: 362-366. 10.1126/science.275.5298.362. Katayama T, Davin LB, Lewis NG: An extraordinary accumulation of (-)-pinoresinol in cell-free extracts of Forsythia intermedia: evidence for enantiospecific reduction of (+)-pinoresinol. Phytochemistry. 1992, 31: 3875-3881. 10.1016/S0031-9422(00)97545-9. Katayama T, Davin LB, Chu A, Lewis NG: Novel benzylic ether reductions in lignan biogenesis in Forsythia intermedia. Phytochemistry. 1993, 33: 581-591. 10.1016/0031-9422(93)85452-W. Umezawa T, Davin LB, Lewis NG: Formation of lignans, (-)-secoisolariciresinol and (-)-matairesinol with Forsythia intermedia cell-free extracts. J Biol Chem. 1991, 266: 10210-10217. Sakakibara N, Suzuki S, Umezawa T, Shimada M: Biosynthesis of yatein in Anthriscus sylvestris. Org Biomol Chem. 2003, 1: 2474-2485. 10.1039/b304411d. Broomhead AJ, Rahman MA, Dewick PM, Jackson DE, Lucas JA: Matairesinol as precursor of Podophyllum lignans. Phytochemistry. 1991, 30: 1489-1492. 10.1016/0031-9422(91)84194-W. Kuhlmann S, Kranz K, Lücking B: Aspects of cytotoxic lignan biosynthesis in suspension cultures of Linum nodiflorum. Phytochem Rev. 2002, 1: 37-43. 10.1023/A:1015876001812. Kamil WM, Dewick PM: Biosynthesis of the lignans α- and β-peltatin. Phytochemistry. 1986, 25: 2089-2092. 10.1016/0031-9422(86)80071-1. van Uden W, Bouma AS, Bracht Waker JF, Middel O, Wichers HJ, Waard PD, Woerdenbag HJ, Kellogg RM, Pras N: The production of podophyllotoxin and its 5-methoxy derivative through bioconversion of cyclodextrin complexed deoxy-podophyllotoxin by plant cell cultures. Plant Cell Tissue Organ Cult. 1995, 42: 73-79. 10.1007/BF00037684. van Uden W, Bos JA, Boeke GM, Woerdenbag HJ, Pras N: The large-scale isolation of deoxypodophyllotoxin from rhizomes of Anthriscus sylvestris followed by its bioconversion into 5-methoxypodophyllotoxin β-d-glucoside by cell cultures of Linun flavum. J Natural Prod. 1997, 60: 401-403. 10.1021/np960748o. Yousefzadi M, Sharifi M, Behmanesh M, Moyano E, Bonfill M, Cusido RM, Palazon J: Podophyllotoxin: current approaches to its biotechnological production and future challenges. Eng Life Sci. 2010, 10: 281-292. 10.1002/elsc.201000027. Singh K, Rani A, Paul A, Dutt S, Joshi R, Gulati A, Ahuja PS, Kumar S: Differential display mediated cloning of anthocyanidin reductase gene from tea (Camellia sinensis) and its relationship with the concentration of epicatechins. Tree Physiol. 2009, 29: 837-846. 10.1093/treephys/tpp022. Kawoosa T, Singh H, Kumar A, Sharma SK, Devi K, Dutt S, Vats SK, Sharma M, Ahuja PS, Kumar S: Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa. Funct Integr Genomics. 2010, 10: 393-404. 10.1007/s10142-009-0152-9. Kumar H, Singh K, Kumar S: 2C-methyl-d-erythritol-2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene. Mol Biol Rep. 2012, 12: 10971-10978. Singh RS, Gara RK, Bhardwaj PK, Kaachra A, Malik S, Kumar R, Sharma M, Ahuja PS, Kumar S: Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]. BMC Mol Biol. 2010, 11: 88-10.1186/1471-2199-11-88. Schuler MA: Plant cytochrome P450 monooxygenases. Crit Rev Plant Sci. 1996, 15: 235-284. 10.1080/07352689609701942. Federolf K, Alfermann AW, Fuss E: Aryltetralin-lignan formation in two different cell suspension cultures of Linum album: Deoxypodophyllotoxin 6-hydroxylase, a key-enzyme in the formation of 6-methoxypodophyllotoxin. Phytochemistry. 2007, 68: 1397-1406. 10.1016/j.phytochem.2007.02.031. Lam KC, Ibrahim RK, Behdad B, Dayanandan S: Structure, function, and evolution of plant O-methyltransferases. Genome. 2007, 50: 1001-1013. 10.1139/G07-077. Lewis NG, Yamamoto E: Lignin: occurrence, biogenesis and biodegradation. Annu Rev Plant Physiol Plant Mol Biol. 1990, 41: 455-496. 10.1146/annurev.pp.41.060190.002323. Boerjan W, Ralph J, Baucher M: Lignin biosynthesis. Annu Rev Plant Biol. 2003, 54: 519-546. 10.1146/annurev.arplant.54.031902.134938. Zhong R, Morrison WH, Himmelsbach DS, Poole FL, Ye ZH: Essential role of caffeoyl coenzyme A O-methyltransferase in lignin biosynthesis in woody poplar plants. Plant Physiol. 2000, 124: 563-578. 10.1104/pp.124.2.563. Ross J, Li Y, Lim E, Bowles DJ: Higher plant glycosyltransferases. Genome Biol. 2001, 2: REVIEWS3004- Vogt T, Jones P: Glycosyltransferases in plant natural product synthesis: characterization of a supergene family. Trends Plant Sci. 2000, 5: 380-386. 10.1016/S1360-1385(00)01720-9. Wang J, Hou B: Glycosyltransferases: key players involved in the modification of plant secondary metabolites. Front Biol China. 2009, 4: 39-46. 10.1007/s11515-008-0111-1. Berim A, Ebel R, Schneider B, Petersen M: UDP-glucose:(6-methoxy) podophyllotoxin 7-O-glucosyltransferase from suspension cultures of Linum nodiflorum. Phytochemistry. 2008, 69: 374-381. 10.1016/j.phytochem.2007.07.030. Jha A, Mehra M, Shankar R: The regulatory epicenter of miRNAs. J Biosci. 2011, 36: 621-638. 10.1007/s12038-011-9109-y. Jha A, Shankar R: miReader: Discovering novel miRNAs in species without sequenced genome. PLoS One. 2013, 8: e66857-10.1371/journal.pone.0066857.