Tramtrack69 interacts with the dMi‐2 subunit of the <i>Drosophila</i> NuRD chromatin remodelling complex

EMBO Reports - Tập 2 Số 12 - Trang 1089-1094 - 2001
Christopher M. Murawsky1, Alexander Brehm2, Paul Badenhorst3, Nicholas J. Lowe1, Peter B. Becker2, Andrew Travers1
1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
2Adolf‐Butenandt‐Institut, Ludwig‐Maximilians‐Universität Schillerstrasse 44 D‐80336 München Germany
3Present address: NCI Laboratory of Molecular Cell Biology, National Institutes of Health Bethesda MD 20892-4255 USA

Tóm tắt

dMi‐2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two‐hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co‐purifies with the dNuRD complex isolated from embryo extracts. dMi‐2 and Ttk69 co‐immunoprecipitate from embryonic extracts, indicating that they can associate in vivo. Both dMi‐2 and Ttk69 co‐localize at a number of discrete sites on polytene chromosomes, showing that they bind common target loci. We also demonstrate that dMi‐2 and Ttk interact genetically, indicating a functional interaction in vivo. We propose that Ttk69 represses some target genes by remodelling chromatin structure through the recruitment of the dNuRD complex.

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EMBO Reports

Tập 2 Số 12

1089-1094

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