Tomographic measurement of local cerebral glucose metabolic rate in humans with (F‐18)2‐fluoro‐2‐deoxy‐D‐glucose: Validation of method

Annals of Neurology - Tập 6 Số 5 - Trang 371-388 - 1979
M. E. Phelps1,2, S.C. Huang1,2, Edward J. Hoffman1,2, Carl Selin1,2, Louis Sokoloff1,2, Dietmar Kuhl1,2
1Division of Nuclear Medicine, UCLA School of Medicine, the Laboratory of Nuclear Medicine and Radiation Biology, University of California, Los Angeles, CA
2Laboratory of Cerebral Metabolism, National Institutes of Health, Bethesda, MD

Tóm tắt

AbstractTracer techniques and quantitative autoradiographic and tissue counting models for measuremnt of metabolic rates were combined with positron computed tomography (PCT) and (F‐18)2‐fluoro‐2‐deoxy‐D‐glucose (FDG) for the measurement of local cerebral metabolic rate for glucose (LCMRGlc) in humans. A three‐compartment model, which incorporates hydrolysis of FDG‐6‐PO4 to FDG, was developed for the measure of kinetic constants and calculation of LCMRGlc. Our model is an extension of that developed by Sokoloff et al. Although small, hydrolysis of FDG‐6‐PO4 was found to be significant. A PCT system, the ECAT, was used to determine the rate constants, lumped constant, and stability of the model in human beings. The data indicate that cerebral FDG‐6‐PO4 in humans increases for about 90 minutes, plateaus, and then slowly decreases. After 10 minutes, cerebral blood FDG activity levels were found to be a minor fraction of tissue activity. Precursor pool turnover rate, distribution volumes, and red blood cell‐plasma concentration ratios were determined. Reproducibility (precision) of LCMRGlc measurements (∼2 cm2 regions) was ± 5.5% over a 5‐hour period. The replacement of arterial blood sampling with venous sampling was validated.

Từ khóa


Tài liệu tham khảo

Anchors JM, 1977, Cerebral glucose‐6‐phosphatase and the movement of 2‐deoxy‐D‐glucose across cell membranes, J Biol Chem, 252, 7035, 10.1016/S0021-9258(19)66931-0

10.1172/JCI104452

10.1172/JCI103324

10.1172/JCI104453

10.1042/bj1280199

Betz AL, 1973, Kinetics of unidirection glucose transport in the isolated dog brain, Am J Physiol, 225, 586, 10.1152/ajplegacy.1973.225.3.586

Buschiazzo PM, 1970, Sugar transport across the blood brain barrier, Am J Physiol, 219, 1505, 10.1152/ajplegacy.1970.219.5.1505

10.1152/jappl.1967.23.2.183

Culter RWP, 1971, Mediated transport of glucose between blood and brain in the cat, Am J Physiol, 220, 1182, 10.1152/ajplegacy.1971.220.5.1182

Fishman RA, 1973, Carrier transport of glucose between blood and cerebral spinal fluid, Am J Physiol, 206, 173

Gallagher BM, 1977, Radiopharmaceutical XXIII. 18F‐labeled glucose metabolism in vivo:tissue distribution and imaging studies in animals, J Nucl Med, 18, 990

Gallagher BM, 1978, Metabolic trapping as a principle of radiopharmaceutical design. The factors responsible for the bio‐distribution of 18F‐2‐deoxy‐2‐fluoro‐D‐glucose (18FDG), J Nucl Med, 19, 1154

10.1016/S0021-9258(18)84948-1

10.1111/j.1471-4159.1975.tb04395.x

10.1007/BF01478536

10.1161/01.RES.43.2.324

10.1001/archneur.1973.00490190056006

10.1161/01.STR.5.5.630

10.1016/0306-4522(78)90106-9

Hers HG, 1957, Le Métabolisme du fructose, 102

Hers HG, 1950, Le Systeme phosphatasique: repartition de l'activité glucose‐6‐phosphatasique dans les tissus, Bull Soc Chim Biol, 32, 20

10.1097/00004728-197906000-00001

HuangSCPhelpsMEHoffmanEJet al: Non invasive determination of local cerebral metabolic rate of glucose in normal man with (F18)2‐fluoro‐2‐deoxyglucose and emission computed tomography: theory and results.Am J Physiol(in press)

Ido T, 1978, Labeled 2‐deoxy‐D‐glucose analogs. 18F‐labeled 2‐deoxy‐2‐fluoro‐D‐glucose, 2‐deoxy‐2‐fluoro‐D‐mannose and 14‐2‐deoxy‐12‐fluoro‐D‐glucose, J Label Compds Radiopharm, 24, 174

Ingvar DH, 1965, Regional cerebral blood flow, Acta Neurol Scand [Suppl], 24, 65

10.1002/ana.410040402

KuhlDEAlaviAHoffmanEJet al: Local cerebral blood volume in head‐injured patients: determination by emission computed tomography of Tc‐99mred cells.J Neurosurg(in press)

Kuhl DE, 1977, Medical Radionuclide Imaging, 309

10.1097/00004728-197811000-00040

Kuhl DE, 1977, Initial clinical experience with 18F‐2‐deoxy‐D‐glucose for determination of local cerebral glucose utilization by emission computed tomography, Acta Neurol Scand, 56, 192

10.1161/01.RES.36.5.610

10.1111/j.1471-4159.1977.tb09610.x

10.1161/01.CIR.7.5.724

Passow H, 1964, The Red Blood Cell, 71

Pennell RB, 1964, The Red Blood Cell, 29

10.1016/S0001-2998(77)80042-1

Phelps ME, 1973, Correlation between PaCO2 and regional cerebral blood volume by x‐ray fluorescence, J Appl Physiol, 35, 274, 10.1152/jappl.1973.35.2.274

10.1152/jappl.1973.35.5.741

Phelps ME, 1977, Positron tomography: an “in vivo” autoradiographic approach to measurement of cerebral hemodynamics and metabolism, Acta Neurol Scand, 56, 446

Phelps ME, 1978, ECAT: a new computerized tomographic imaging system for positron emitting radiopharmaceuticals, J Nucl Med, 19, 635

Phelps ME, 1977, Medical Radionuclide Imaging, 233

Phelps ME, 1978, Investigation of (18F)‐2‐fluoro‐2‐deoxyglucose for the measure of myocardial glucose metabolism, J Nucl Med, 19, 1311

10.1097/00004728-197811000-00042

10.1210/endo-82-1-1

10.3181/00379727-105-26150

Raichle ME, 1975, In vivo measurement of brain glucose transport and metabolism employing glucose‐11C, Am J Physiol, 228, 1936, 10.1152/ajplegacy.1975.228.6.1936

10.1126/science.414358

Reivich M, 1977, Measurement of local cerebral glucose metabolism in man with 18F‐2‐fluoro‐2‐deoxy‐D‐glucose, Acta Neurol Scand, 56, 192

10.1161/01.RES.44.1.127

Robinson GD, 1978, F‐18 fluorodeoxyglucose: remote, semi automated production using a compact cyclotron (abstract), J Nucl Med, 19, 701

10.1172/JCI102150

Smith AL, 1971, Effect of arterial CO2 tension on cerebral blood flow, mean transit time and vascular volume, J Appl Physiol, 31, 701, 10.1152/jappl.1971.31.5.701

10.1111/j.1471-4159.1977.tb10649.x

10.1016/0006-8993(76)90011-1

10.1097/00000542-197201000-00013

Weiner RS, 1955, The use of “arterialized” blood for the determination of arterial O2 and CO2 tensions, J Thorac Surg, 30, 683, 10.1016/S0096-5588(20)30592-4

Yudilevich DL, 1971, Blood‐brain transfer of glucose and other molecules measured by lipid indicator dilution, Am J Physiol, 220, 841, 10.1152/ajplegacy.1971.220.3.841

Thông tin
Thông tin xuất bản

Annals of Neurology

Tập 6 Số 5

371-388

Thông tin tác giả