Tissue‐specific expression of dentin sialophosphoprotein (DSPP) and its polymorphisms in mouse tissues

Cell Biology International - Tập 33 - Trang 816-829 - 2009
Guohua Yuan1,2, Yinghua Wang1, Jelica Gluhak-Heinrich3, Guobin Yang1, Lei Chen1, Tong Li1, Li-An Wu1, Zhi Chen4, Mary MacDougall5, Shuo Chen1
1Department of Pediatric Dentistry, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA
2Key Laboratory of Oral Biomedical Engineering Ministry of Education, Wuhan University School and Hospital of Stomatology, Wuhan, China
3Department of Orthopedics, Dental School, The University of Texas Health Science Center at San Antonio, TX, USA
4Department of Cariology and Endodontics, Wuhan University School and Hospital of Stomatology, Wuhan, China
5Department of Oral/Maxillofacial Surgery, University of Alabama, School of Dentistry, 1919 7th Avenue South, SDB 702, Birmingham, AL 35294-0007, USA

Tóm tắt

AbstractDentin sialophosphoprotein (DSPP) consists of dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). DSPP is highly expressed in mineralized tissues. However, recent studies have shown that DSPP is also expressed in several active metabolic ductal epithelial tissues and exists in a variety of sequences. We have investigated DSPP expression in various mouse tissues using RT‐PCR, in situ hybridization and immunohistochemical analyses. To identify DSPP gene polymorphisms, we screened a mouse tooth cDNA library as well as isolated and characterized DSPP variations. Our results show that DSPP is predominantly expressed in teeth and moderately in bone tissues. We also have characterized a full‐length DSPP cDNA clone with an open‐reading frame of 940 codons and this polyadenylation signal. Compared to previously reported mouse DSPP cDNAs, 13 sequence variations were identified, including 8 non‐synonymous single nucleotide polymorphisms and an in‐frame indel (8 amino acids) at DPP domain of the mouse DSPP. These 8 amino acids are rich in aspartic acid and serine residues. Northern blot assay showed a prominent band at 4.4 kb. RT‐PCR demonstrated that this mouse DSPP gene was dominantly expressed in teeth. The predicted secondary structure of DPP domain of this DSPP showed differences from the previously published mouse DPPs, implying that they play different roles during tooth development and formation.

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Cell Biology International

Tập 33

816-829

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