Tissue‐specific and developmental expression of human transthyretin gene in transgenic mice

Wiley - Tập 8 Số 4 - Trang 195-205 - 1987
Ken‐Ichi Yamamura1, Shoji Wakasugi1, Shuichiro Maeda2, Takeaki Inomoto1, T. Iwanaga1, Masahiro Uehira1, Kimi Araki1, Jun‐ichi Miyazaki1, Kazunori Shimada2
1Institute for Medical Genetics, Kumamoto University Medical School, Kumamoto Japan
2Department of Biochemistry, Kumamoto University Medical School, Kumamoto, Japan

Tóm tắt

AbstractTo analyze the regulation of transthyretin gene expression we have produced transgenic mice by microinjecting cloned human transthyretin genes into fertilized eggs of C57BL/6 mice. The 7.6‐kilobase (kb) human transthyretin gene containing about 500 base pairs (bp) in the upstream region was used for microinjection. Seven out of nine transgenic mice had detectable amounts of human transthyretin in serum when analyzed by enzyme‐linked immunosorbent assay.Transthyretin mRNA was detected in liver and yolk sac but not in other tissues including brain. The amount of mRNA was variable among transgenic mice and was about one‐tenth of mouse endogenous transthyretin mRNA. Human and mouse transthyretin mRNAs were detected in liver of fetus and yolk sac at 13 days of gestation and unlike yolk sac the level of mRNA in liver increased gradually during development and reached the maximum at around 17 days of gestation. Human transthyretin was associated with mouse transthyretin to form tetramers as judged from the dilution curve of enzyme‐linked immu‐nosorbent assay and the spur formation in Ouchterlony assay.

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Wiley

Tập 8 Số 4

195-205

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