Three sympatrically occurring species of Metarhizium show plant rhizosphere specificity

Microbiology (United Kingdom) - Tập 157 Số 10 - Trang 2904-2911 - 2011
Michael Wyrebek1, Cristina Huber1, Ramanpreet Kaur Sasan1, Michael Bidochka1
1Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, ON L2S 3A1, Canada

Tóm tắt

Here we tested the hypothesis that species of the soil-inhabiting insect-pathogenic fungusMetarhiziumare not randomly distributed in soils but show plant-rhizosphere-specific associations. We isolatedMetarhiziumfrom plant roots at two sites in Ontario, Canada, sequenced the 5′ EF-1α gene to discernMetarhiziumspecies, and developed an RFLP test for rapid species identification. Results indicated a non-random association of threeMetarhiziumspecies (Metarhizium robertsii,Metarhizium brunneumandMetarhizium guizhouense) with the rhizosphere of certain types of plant species (identified to species and categorized as grasses, wildflowers, shrubs and trees).M. robertsiiwas the only species that was found associated with grass roots, suggesting a possible exclusion ofM. brunneumandM. guizhouense. Supporting this,in vitroexperiments showed thatM. robertsiiconidia germinated significantly better inPanicum virgatum(switchgrass) root exudate than didM. brunneumorM. guizhouense.M. guizhouenseandM. brunneumonly associated with wildflower rhizosphere when co-occurring withM. robertsii. With the exception of these co-occurrences,M. guizhouensewas found to associate exclusively with the rhizosphere of tree species, predominantlyAcer saccharum(sugar maple), whileM. brunneumwas found to associate exclusively with the rhizosphere of shrubs and trees. These associations demonstrate that different species ofMetarhiziumassociate with specific plant types.

Từ khóa


Tài liệu tham khảo

10.1614/WS-08-151.1

10.1351/PAC-CON-09-02-09

10.1128/AEM.67.3.1335-1342.2001

10.1111/j.1462-5822.2005.00823.x

10.3852/mycologia.98.5.737

10.3852/07-202

Bridge, 1993, Morphological, biochemical and molecular characteristics of Metarhizium anisopliae and M. flavoviride, J Gen Microbiol, 139, 1163, 10.1099/00221287-139-6-1163

10.1023/A:1018387918686

10.1016/j.biocontrol.2004.09.003

10.1007/s10526-009-9236-7

10.1128/AEM.00584-08

10.1007/s005720050280

10.1139/w06-014

Fegan, 1993, Random amplified polymorphic DNA markers reveal a high degree of genetic diversity in the entomopathogenic fungus Metarhizium anisopliae var. anisopliae, J Gen Microbiol, 139, 2075, 10.1099/00221287-139-9-2075

Felsenstein, 2009, phylip (phylogeny inference package) version 3.69. Distributed by the author

10.1104/pp.109.147231

10.1128/AEM.68.12.6383-6387.2002

Hunter, 2001, Aerial treatment of the Australian plague locust, Chortoicetes terminifera (Orthoptera: Acrididae) with Metarhizium anisopliae (Deuteromycotina: Hyphomycetes), Bull Entomol Res, 91, 93, 10.1079/BER200080

10.1890/08-1291.1

10.1034/j.1600-0706.2003.12415.x

Klironomos, 2000, Host-specificity and functional diversity among arbuscular mycorrhizal fungi, Microbial Biosystems: New Frontiers. Proceedings of the 8th International Symposium on Microbial Ecology, 845

10.1093/bioinformatics/btm404

10.1016/S0953-7562(09)80436-X

10.1017/S0953756296002560

10.1128/AEM.02287-08

10.4039/entm129171265-1

10.1146/annurev.ento.46.1.667

10.1016/S0261-2194(02)00221-1

10.1016/j.pbi.2009.05.007

10.1128/AEM.67.7.3046-3052.2001

10.1007/978-1-4020-5933-9_34

Molina, 1992, Specificity phenomena in mycorrhizal symbioses: community-ecological consequences and practical implications, Mycorrhizal Functioning, 357

Pinior, 1999, Plants colonized by AM fungi regulate further root colonization by AM fungi through altered root exudation, Can J Bot, 77, 891

10.1016/j.soilbio.2007.10.005

10.1007/s005720100115

10.3852/mycologia.97.1.84

10.1007/BF00437383

Shah, 2003, Entomopathogenic fungi as biological control agents, Appl Microbiol Biotechnol, 61, 413, 10.1007/s00253-003-1240-8

10.1111/j.1399-3054.2007.00983.x

10.1007/s00284-003-4228-9

10.1111/j.1365-294X.2007.03225.x

10.1016/0022-2011(92)90159-2

10.1006/jipa.1995.1031

10.1046/j.1365-294x.1998.00410.x

10.1016/j.funeco.2009.05.001

10.1007/s00572-002-0219-0

10.1128/EC.00409-06

10.1111/j.1365-2745.2008.01389.x

Thông tin
Thông tin xuất bản

Microbiology (United Kingdom)

Tập 157 Số 10

2904-2911

Thông tin tác giả