Three new species of Paraboea (Gesneriaceae) from limestone karsts of China based on morphological and molecular evidence
Tóm tắt
The limestone karsts of Southeast Asia and South China are a major biodiversity hotspot of global terrestrial biomes. With more than 130 described species, Paraboea has become one of the most characteristic plant groups in the Southeast Asian limestone flora. During the course of extensive field work on the limestone formations of southern and southwestern China, three unknown species of Paraboea were collected. Molecular phylogenetic analyses based on nuclear ITS and chloroplast trnL-F sequences strongly confirm the placements of the three new species in Paraboea sensu Puglisi et al. (Taxon 65:277–292.
https://doi.org/10.12705/652.5
, 2016). Moreover, these three novelties can be distinguished from known Paraboea species with distinct morphological characters, further supporting their recognition as new species. With the support of detailed morphological studies and molecular phylogenetic analyses, Paraboea dushanensis, P. sinovietnamica and P. xiangguiensis are recognized as three species new to science.
Tài liệu tham khảo
Burtt BL (1984) Studies in the Gesneriaceae of the Old World: XLVII. Revised generic concepts for Boea and its allies. Notes R Bot Gard Edinb 41:401–452
Chen W-H, Möller M, Shui Y-M, Zhang M-D (2008) A new species of Paraboea (Gesneriaceae) from a karst cave in Guangxi, China, and observations on variations in flower and inflorescence architecture. Bot J Linn Soc 158:681–688. https://doi.org/10.1111/j.1095-8339.2008.00873.x
Chen W-H, Möller M, Zhang M-D, Shui Y-M (2012) Paraboea hekouensis and P. manhaoensis, two new species of Gesneriaceae from China. Ann Bot Fenn 49:179–187. https://doi.org/10.5735/085.049.0304
Chung K-F, Leong W-C, Rubite RR, Repin R, Kiew R, Liu Y, Peng C-I (2014) Phylogenetic analyses of Begonia sect. Coelocentrum and allied limestone species of China shed light on the evolution of Sino-Vietnamese karst flora. Bot Stud 55:e1. https://doi.org/10.1186/1999-3110-55-1
Clements R, Sodhi NS, Schilthuizen M, Ng PKL (2006) Limestone karsts of Southeast Asia: imperiled arks of biodiversity. Bioscience 56:733–742
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797. https://doi.org/10.1093/Nar/Gkh340
Guo J, Pan B, Liu J, Xu W-B, Chung K-F (2015) Three new species of Primulina (Gesneriaceae) from limestone karsts of China based on morphological and molecular evidence. Bot Stud 56:1–13. https://doi.org/10.1186/s40529-015-0115-5
Guo J, Lu Z-C, Liu J, Xu W-B (2016) Paraboea crassifila, a new species of Paraboea (Gesneriaceae) from Danxia landform in Guangxi, China. Taiwania 61:8–12. https://doi.org/10.6165/tai.2016.61.8
He H, Zhang L-B (2011) Polystichum cavernicola, sp. nov. (sect. Haplopolystichum, Dryopteridaceae) from a karst cave in Guizhou, China and its phylogenetic affinities. Bot Stud 52:121–127
Hou M-F, López-Pujol J, Qin H-N, Wang L-S, Liu Y (2010) Distribution pattern and conservation priorities for vascular plants in Southern China: Guangxi Province as a case study. Bot Stud 51:377–386
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649. https://doi.org/10.1093/bioinformatics/bts199
Kiew R (2010) Two new species of Paraboea (Gesneriaceae) from peninsular Malaysia and Thailand. Edinb J Bot 67:209–217. https://doi.org/10.1017/S0960428610000107
Li Z-Y, Wang Y-Z (2004) Plants of Gesneriaceae in China. Henan Science and Technology Publishing House, Zhengzhou
Liu Y, Kono Y, Lin C-R, Xu W-B, Peng C-I (2011) Aspidistra erecta (Asparagaceae), a new species from limestone areas in Guangxi, China. Bot Stud 52:367–373
Liu J, Huang J, Xu WB, Lin CR (2016) Aspidistra ronganensis (Asparagaceae), a new species from limestone areas in Guangxi, China. Phytotaxa 270:63–68. https://doi.org/10.11646/phytotaxa.270.1.7
López-Pujol J, Zhang F-M, Sun H-Q, Ying T-S, Ge S (2011) Centres of plant endemism in China: places for survival or for speciation? J Biogeogr 38:1267–1280. https://doi.org/10.1111/j.1365-2699.2011.02504.x
Middleton DJ, Puglisi C, Triboun P, Möller M (2010) Proposal to conserve Paraboea against Phylloboea and Trisepalum (Gesneriaceae). Taxon 59:1603
Möller M, Cronk QCB (1997) Origin and relationships of Saintpaulia (Gesneriaceae) based on ribosomal DNA internal transcribed spacer (ITS) sequences. Am J Bot 84:956–965
Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858. https://doi.org/10.1038/35002501
Peng C-I, Kuo S-M, Yang H-A, Leong W-C, Liu Y, Nguyen TH, Kono Y, Chung K-F (2014) Two new species of Begonia sect. Coelocentrum, B. guixiensis and B. longa, from Sino-Vietnamese limestone karsts. Bot Stud 55:e52. https://doi.org/10.1186/s40529-014-0052-8
Peng C-I, Yang H-A, Kono Y, Jung M-J, Nguyen TH (2015) Four new species of Begonia (Begoniaceae) from Vietnam: B. abbreviata, B. calciphila, B. sphenantheroides and B. tamdaoensis. Phytotaxa 222:83–99. https://doi.org/10.11646/phytotaxa.222.2.1
Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256. https://doi.org/10.1093/molbev/msn083
Puglisi C, Middleton DJ, Triboun P, Möller M (2011) New insights into the relationships between Paraboea, Trisepalum, and Phylloboea (Gesneriaceae) and their taxonomic consequences. Taxon 60:1693–1702
Puglisi C, Suddee S, Triboun P, Middleton DJ (2015) A new species of Paraboea (Gesneriaceae). Gard Bull Singap 67:101–106
Puglisi C, Yao TL, Milne R, Moller M, Middleton DJ (2016) Generic recircumscription in the Loxocarpinae (Gesneriaceae), as inferred by phylogenetic and morphological data. Taxon 65:277–292. https://doi.org/10.12705/652.5
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542. https://doi.org/10.1093/sysbio/sys029
Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771. https://doi.org/10.1080/10635150802429642
Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109
Tan Y-H, Liu Y-N, Jiang H, Zhu X-X, Zhang W, Yu S-X (2015) Impatiens pandurata (Balsaminaceae), a new species from Yunnan, China. Bot Stud 56:e29. https://doi.org/10.1186/s40529-015-0108-4
Thiers B (2017) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/science/ih/. Accessed 10 Oct 2017
Triboun P (2013) Paraboea middletonii (Gesneriaceae), a new species from Thailand. Thai For Bull 41:45–47
Triboun P, Middleton DJ (2012) Twenty new species of Paraboea (Gesneriaceae) from Thailand. Gard Bull Singap 64:333–370
Triboun P, Middleton DJ (2015) Three new species of Paraboea (Gesneriaceae) from Thailand. Thai For Bull 43:18–23
Wang W-T, Pan K-Y, Li Z-Y (1990) Gesneriaceae. In: Wang W-T (ed) Flora Reipublicae Popularis Sinicae, vol 69. Science Press, Beijing, pp 125–581
Wang W-T, Pan K-Y, Li Z-Y, Weitzman AL, Skog LE (1998) Gesneriaceae. In: Wu Z-Y, Raven PH (eds) Flora of China, vol 18. Science Press, Beijing, pp 244–401
Wang W, Li H-L, Chen Z-D (2014) Analysis of plastid and nuclear DNA data in plant phylogenetics—evaluation and improvement. Sci China Life Sci 57:280–286. https://doi.org/10.1007/s11427-014-4620-7
Wei Y-G (2010) Gesneriaceae of South China. Guangxi Science and Technology Publishing House, Guilin
Wei Y-G, Monro AK, Wang W-T (2011) Addition to the flora of China: seven new species of Elatostema (Urticaceae) from the karst landscapes of Guangxi and Yunnan. Phytotaxa 29:1–27
Wen F, Wei Y-G (2016) Paraboea yunfuensis: a new calcicolous species of Gesneriaceae from Yunfu, Guangdong Province, China. Telopea 19:125–129. https://doi.org/10.7751/telopea10316
Wen F, Hong X, Chen LY, Zhou SB, Wei YG (2013) A new species of Paraboea (Gesneriaceae) from a karst limestone hill in southwestern Guangdong, China. Phytotaxa 131:1–8. https://doi.org/10.11646/phytotaxa.131.1.1
Xu Z, Burtt BL, Skog LE, Middleton DJ (2008) A revision of Paraboea (Gesneriaceae). Edinb J Bot 65:161–347. https://doi.org/10.1017/S0960428608005106
Xu W-B, Huang Y-S, Wei G-F, Tan W-N, Liu Y (2012a) Paraboea angustifolia (Gesneriaceae): a new species from limestone areas in northern Guangxi, China. Phytotaxa 62:39–43. https://doi.org/10.11646/phytotaxa.62.1.8
Xu W-B, Pan B, Liu Y, Peng C-I, Chung K-F (2012b) Two new species, Primulina multifida and P. pseudomollifolia (Gesneriaceae), from karst caves in Guangxi, China. Bot Stud 53:165–175
Xu W-B, Meng T, Zhang Q, Wu W-H, Liu Y, Chung K-F (2014) Petrocodon (Gesneriaceae) in the limestone karsts of Guangxi, China: three new species and a new combination based on morphological and molecular evidence. Syst Bot 39:965–974. https://doi.org/10.1600/036364414X681437
Yu S-X, Janssens SB, Zhu X-Y, Lidén M, Gao T-G, Wang W (2015) Phylogeny of Impatiens (Balsaminaceae): integrating molecular and morphological evidence into a new classification. Cladistics 32:179–197. https://doi.org/10.1111/cla.12119
Zhang LB, He H (2011) Polystichum fengshanense, sp. nov. (sect. Haplopolystichum, Dryopteridaceae) from Karst caves in Guangxi, China based on morphological, palynological, and molecular evidence. Syst Bot 36:854–861. https://doi.org/10.1600/036364411x604877