Three Hen Strains Fed Photoisomerized trans,trans CLA-Rich Soy Oil Exhibit Different Yolk Accumulation Rates and Source-Specific Isomer Deposition
Tóm tắt
Most CLA chicken feeding trials used cis,trans (c,t) and trans,cis (t,c) CLA isomers to produce CLA-rich eggs, while reports of trans,trans (t,t) CLA enrichment in egg yolks are limited. The CLA yolk fatty acid profile changes and the 10–12 days of feeding needed for maximum CLA are well documented, but there is no information describing CLA accumulation during initial feed administration. In addition, no information on CLA accumulation rates in different hen strains is available. The aim of this study was to determine a mathematical model that described yolk CLA accumulation and depletion in three hen strains by using t,t CLA-rich soybean oil produced by photoisomerization. Diets of 30-week Leghorns, broilers, and jungle fowl were supplemented with 15 % CLA-rich soy oil for 16 days, and eggs were collected for 32 days. Yolk fatty acid profiles were measured by GC-FID. CLA accumulation and depletion was modeled by both quadratic and piecewise regression analysis. A strong quadratic model was proposed, but it was not as effective as piecewise regression in describing CLA accumulation and depletion. Broiler hen eggs contained the greatest concentration of CLA at 3.2 mol/100 g egg yolk, then jungle fowl at 2.9 mol CLA, and Leghorns at 2.3 mol CLA. The t,t CLA isomer levels remained at 55 % of total yolk CLA during CLA feeding. However, t-10,c-12 (t,c) CLA concentration increased slightly during CLA accumulation and was significantly greater than c-9,t-11 CLA. Jungle fowl had the smallest increase in yolk saturated fat with CLA yolk accumulation.
Tài liệu tham khảo
Chamruspollert M, Sell JL (1999) Transfer of dietary conjugated linoleic acid to egg yolks of chicken hens. Poult Sci 78:1138–1150
Schaefer K (2001) Incorporation of dietary linoleic and conjugated linoleic acids and related effects on eggs of laying hens. Lipids 36:1217–1222
Grobas S, Mendez J, Lazaro R, de Bias C, Mateos GG (2001) Influence of source and percentage of fat added to diet on performance and fatty acid composition of egg yolks of two strains of laying hens. Poult Sci 80:1171–1179
Du M, Ahn DU, Sell JL (1999) Effects of dietary conjugated linoleic acid on the composition of egg yolk lipids. Poult Sci 78:1639–1645
Yin JD, Shang XG, Li DF, Wang FL (2008) Effects of dietary conjugated Linoleic acid on the fatty acid profile and cholesterol content of egg yolks from different breeds of layers. Poult Sci 87:284–290
Szymczyk B, Pisulewski PM, Szczurek W, Hanczakowski P (2001) Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br J Nutr 85:465–473
Watkins BA, Devitt AA, Feng S (2001) Designed eggs containing conjugated linoleic acids and omega-3 polyunsaturated fatty acids. World Rev Nutr Diet 90:162–182
Islam MA, Kim YS, Oh TW, Kim GS, Won CK, Kim HG, Choi MS, Kim JO, Ha YL (2010) Superior anticarcinogenic activity of trans,trans-conjugated linoleic acid in N-methyl-N-nitrosourea- induced rat mammary tumorigenesis. J Agric Food Chem 58:5670–5678
Beppu F, Hosokawa M, Tanaka L, Kohno H, Tanaka T, Mi-yashita K (2006) Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells. J Nutr Biochem 17:830–883
Islam MA, Kim YS, Jang WJ, Lee SM, Kim HG, Kim SY, Kim JO, Ha YL (2008) A mixture of trans,trans conjugated linoleic acid induces apoptosis in MCF-7 human breast cancer cells with reciprocal expression of bax and Bcl-2. J Agric Food Chem 56:5970–5976
Shah U, Baum JI, Proctor A (2013) A new perspective on trans,trans conjugated linoleic acid (CLA), an overlooked CLA isomer. J Am Oil Chem Soc. doi:10.1007/s11746-013-2382
Shinn S, Liyanage R, Lay J, Proctor A (2014) Improved fatty acid analysis of conjugated linoleic acid-rich egg yolk triacylglycerols and phospholipid species. J Agric Food Chem. doi:10.1021/jf501100y
Jain VP, Proctor A, Lall R (2008) Pilot-scale production of conjugated linoleic acid- rich soy oil by photoirradiation. J Food Sci 73:183–192
Gilbert W, Gadang V, Proctor A, Jain V, Devareddy L (2011) trans–trans Conjugated linoleic acid enriched soybean oil reduces fatty liver and lowers serum cholesterol in obese Zucker rats. Lipids 46:961–968
Tokle T, Jain VP, Proctor A (2009) Effect of minor oil constituents on soy oil conjugated linoleic acid production. J Agric Food Chem 57:8989–8997
Shinn SE, Proctor A (2013) Rapid lipid extraction from egg yolks. J Amer Oil Chem Soc 90:367–369
Lall RK, Proctor A, Jain VP (2009) A rapid, micro FAME preparation method for vegetable oil fatty acid analysis by gas chromatography. J Am Oil Chem Society 86:309–314
Shah U, Proctor A, Lay JO Jr, Moon K (2012) Determination of CLA trans,trans positional isomerism in CLA-rich soy oil by GC–MS and silver ion HPLC. J Am Oil Chem Soc 89:979–985
Hara A, Radin NS (1978) Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem 90:420–426
Sessler AM, Ntambi JM (1998) Polyunsaturated fatty acid regulation of gene expression. J Nutr 128:923–926
Cherian G, Ai W, Goeger MP (2005) Maternal dietary conjugated linoleic acid alters hepatic triacylglycerol and tissue fatty acids in hatched chicks. Lipids 40(2):131–136
Cherian G, Holsonbake TB, Goeger MP, Bildfell R (2002) Dietary CLA alters yolk and tissue FA composition and hepatic histopathology of laying hens. Lipids 37:751–757