Thiamine-dependent Accumulation of Tetramethylpyrazine Accompanying a Mutation in the Isoleucine-Valine Pathway

Journal of Bacteriology - Tập 94 Số 2 - Trang 323-326 - 1967
A. L. Demain1, M. Jackson1, Nelson R. Trenner1
1Merck Sharp & Dohme Research Laboratories, Merck & Co., Inc., Rahway, New Jersey 07065

Tóm tắt

A mutant of Corynebacterium glutamicum was found to accumulate high concentrations of a material which crystallized upon cooling of the broth. The compound was identified as tetramethylpyrazine. The mutant was found to require isoleucine, valine, leucine, and pantothenate for growth. All four requirements probably result from the loss of a single enzyme of the isoleucine-valine pathway. Since similar mutants of Neurospora crassa accumulate acetoin, the present mutant probably forms tetramethylpyrazine from acetoin. Accumulation of tetramethylpyrazine was dependent upon addition of thiamine. This observation is consistent with the known activity of diphosphothiamine as a cofactor for the formation of acetolactate (a precursor of acetoin) from pyruvate.

Từ khóa


Tài liệu tham khảo

ADACHI , T., H. KAMIYA , A ND T . KOSUGE. 1964 . Studies on the metabolic products of Bacillus subtilis. IV. Determination and mechanisms of formation of tetramethylpyrazine . J. Pharm. Soc. Japan 84 : 545 - 548 .

HALPERN , Y. S. , AND H. E. UMBARGER. 1959 . Evidence for two distinct enzyme systems forming acetolactate in Aerobacter aerogenes . J. Biol. Chem. 234 : 3067 - 3071 .

KINOSHrIA S. 1963. Amino acids p. 206-226. In C. Rainbow and A. H. Rose [ed.] Biochemistry of industrial microorganisms. Academic Press Inc. New York.

KIPPING , F. B. 1929 . The stereoisomeric 2:3:5:6- tetramethyl piperazines. I . J. Chem. Soc., p.2889-2897.

KLEIN B. AND J. BERKOWITZ. 1959. Pyrazines. J. BAC-TERIOL.

KOSUGE , T. , AND H. KAMIYA. 1962 . Discovery of a pyrazine in a natural product: tetramethylpyrazine from cultures of a strain of Bacillus subtilis . Nature 193 : 776 .

MOYED , H. S. 1964 . Inhibition of the biosynthesis of the pyrimidine portion of thiamine by adenosine . J. Bacteriol. 88 : 1024 - 1029 .

UMBARGER , E. , AND B. BROWN. 1958 . Isoleucine and valine metabolism in Escherichia coli. VIII. The formation of acetolactate . J. Biol. Chem. 233 : 1156 - 1160 .

UMBARGER E. AND B. D. DAVis. 1962. Pathways of amino acid biosynthesis p. 167-251. In I. C. Gunsalus and R. Y. Stanier [ed.] The bacteria a treatise on structure and function vol. 3. Academic Press Inc. New York.

WAGNER , R. P. , A. BERGQUIST , T. BARBEE , A ND K . KIRITANI. 1964 . Genetic blocks in the isoleucine-valine pathway of Neurospora crassa . Genetics 49 : 865 - 882 .

WAGNER , R. P. , A. BERGQUIST , A ND H . S. FORREST. 1959 . The accumulation of acetylmethylcarbinol and acetylethylcarbinol by a mutant of Neurospora crassa and its significance in the biosynthesis of isoleucine and valine . J. Biol. Chem. 234 : 99 - 104 .