Therapeutic Angiogenesis Using Basic Fibroblast Growth Factor in Combination with a Collagen Matrix in Chronic Hindlimb Ischemia

Hindawi Limited - Tập 2012 - Trang 1-8 - 2012
Jianyin Zhou1, Yilin Zhao1, Jinling Wang2, Sheng Zhang1, Zhengjin Liu3, Maochuan Zhen1, Yun Liu1, Pingguo Liu1, Zhenyu Yin1, Xiaomin Wang1,4
1Department of Hepatobiliary Surgery, Zhongshan Hospital, Xiamen University, Xiamen 361001, China
2Department of Emergency, Zhongshan Hospital, Xiamen University, Xiamen, 361001, China
3Department of Pathology Zhongshan Hospital, Xiamen University, Xiamen 361001, China
4Digestive Institute of Xiamen University, Xiamen 361001, China

Tóm tắt

Although therapeutic angiogenesis by angiogenic cytokines is a feasible strategy to improve regional blood flow in ischemic regions, the optimal delivery mode needs to be established. Here we designed a complex of collagen matrix (CM) and basic fibroblast growth factor (bFGF) and evaluated its proangiogenic effect in ischemic hindlimbs. The bFGF-CM was prepared using lyophilization. The morphology, porosity and toxicity of CM were examined. The bFGF releasing profile and bioactivity of released bFGF were assessed. bFGF-CM was intramuscularly implanted into the rabbit ischemic hindlimb model. Oxygen saturation parameters (OSP) of ischemic hindlimbs was measured to evaluate the extremity perfusion at intervals. Histological examination was performed to evaluate the level of angiogenesis. The CM and bFGF-CM were of identical multiporous structure lacking cytotoxicity. The releasing profile lasted 10 days and the released bFGF remained bioactive. OSP in bFGF-CM group was significantly higher than that in CM, bFGF and ischemic groups at 2 and 4 weeks. The number of capillaries and mature vessels in bFGF-CM group were significantly greater than that in untreated control, CM and bFGF groups. Therefore, bFGF-CM enables the safe and effective long-term release of bFGF with improved angiogenesis in ischemic hindlimbs compared with CM devoid of bFGF.

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Hindawi Limited

Tập 2012

1-8

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