The zebrafish, brain‐specific, aromatase <i>cyp19a2</i> is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation

Developmental Dynamics - Tập 236 Số 11 - Trang 3155-3166 - 2007
Evangelia Kallivretaki1, Carlo R. Largiadèr2, Stephan C. F. Neuhauss3, Olivier Kah4, Helmut Segner1
1Centre for Fish and Wildlife Health, University of Bern, Bern, Switzerland
2Eawag, Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland
3Institute of Zoology, University of Zurich, Zurich, Switzerland
4Endocrinologie Moléculaire de la Reproduction, Unité Mixte de Recherche 6026, Centre National de la Recherche Scientifique, Université de Rennes 1, Rennes France

Tóm tắt

AbstractDifferential cyp19 aromatase expression during development leads to sexual dimorphisms in the mammalian brain. Whether this is also true for fish is unknown. The aim of the current study has been to follow the expression of the brain‐specific aromatase cyp19a2 in the brains of sexually differentiating zebrafish. To assess the role of cyp19a2 in the zebrafish brain during gonadal differentiation, we used quantitative reverse transcriptase‐polymerase chain reaction and immunohistochemistry to detect differences in the transcript or protein levels and/or expression pattern in juvenile fish, histology to monitor the gonadal status, and double immunofluorescence with neuronal or radial glial markers to characterize aromatase‐positive cells. Our data show that cyp19a2 expression levels during zebrafish sexual differentiation cannot be assigned to a particular sex; the expression pattern in the brain is similar in both sexes and aromatase‐positive cells appear to be mostly of radial glial nature. Developmental Dynamics 236:3155–3166, 2007. © 2007 Wiley‐Liss, Inc.

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Developmental Dynamics

Tập 236 Số 11

3155-3166

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