The use of desiccation to treat Staphylococcus aureus biofilm‐infected wounds

Wound Repair and Regeneration - Tập 24 Số 2 - Trang 394-401 - 2016
Eugene Park1,2, Sarah A. Long1,2, Akhil K. Seth1, Matthew R. Geringer1, Wei Xu1, Claudia Chávez‐Muñoz1, Kai P. Leung3, Seok Jong Hong1, Robert D. Galiano1, Thomas A. Mustoe1
1Division of Plastic Surgery, Northwestern University Feinberg School of Medicine, Chicago, Illinois
2these authors contributed equally to this work
3Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, Texas

Tóm tắt

AbstractChronic wounds colonized with biofilm present a major burden to our healthcare system. While the current paradigm for wound healing is to maintain a moist environment, we sought to evaluate the effects of desiccation, and the ability of honey to desiccate wounds, on wound healing characteristics in Staphylococcus aureus biofilm wounds. In vivo biofilm wound healing after exposure to open‐air desiccation, honey, molasses, and saline was analyzed using a rabbit ear model of S. aureus biofilm wounds previously developed by our group. Wound morphology was examined using scanning electron microscopy and granulation tissue deposition was measured using light microscopy with hematoxylin and eosin staining. Viable bacterial counts in rabbit ear biofilm wounds and scabs were measured using a drop dilution method. In vitro S. aureus growth curves were established using tryptic soy broth containing honey and glycerol. Gene expression analysis of rabbit ear wounds was performed using reverse transcription quantitative PCR. Rabbit ear S. aureus biofilm wounds exposed to open‐air desiccation, honey, and molasses developed a dry scab, which displaced the majority of biofilm bacteria off of the wound bed. Wounds treated with open‐air desiccation, honey, and molasses expressed lower levels of the inflammatory markers tumor necrosis factor‐α and interleukin‐1β at postoperative day 12 compared with wounds treated with saline, and had increased levels of granulation tissue formation. In vitro growth of S. aureus in tryptic soy broth was inhibited by the presence of honey to a greater extent than by the presence of osmolality‐matched glycerol. Desiccation of chronic wounds colonized with biofilm via exposure to open air or honey leads to improved wound healing by decreasing bacterial burden and inflammation, and increasing granulation tissue formation. The ability of honey to help heal chronic wounds is at least in part due to its ability to desiccate bacterial biofilm, but other factors clearly contribute.

Từ khóa


Tài liệu tham khảo

10.1111/j.1524-475X.2009.00543.x

10.1056/NEJMra055289

10.1016/S0140-6736(05)67700-8

10.1371/journal.pone.0059979

10.1001/jama.299.22.2682

10.1128/JCM.00501-08

10.1111/j.1524-475X.2007.00321.x

10.1126/science.295.5559.1487

10.1073/pnas.0610226104

10.1111/j.1524-475X.2011.00690.x

10.1111/j.1524-475X.2009.00489.x

10.1111/j.1524-475X.2012.00764.x

10.1097/PRS.0b013e31823aeb3b

10.1001/archsurg.1994.01420340057010

10.1038/193293a0

10.1038/200377a0

10.1016/j.clindermatol.2006.12.005

10.1086/590972

Herszage L, 1980, Tratamiento de las heridas supuradas con azùcar granulado comercial, Bol Trab Soc Argent Cir, 41, 315

10.1128/AAC.23.5.766

10.1016/S0140-6736(82)92065-7

10.1016/S0140-6736(85)91498-9

10.1016/0140-6736(91)91135-H

10.1093/jac/dki193

10.1371/journal.pone.0018229

10.1016/j.carres.2007.12.011

10.1002/mnfr.200700282

Jull AB, 2013, Honey as a topical treatment for wounds, Cochrane Database Syst Rev, 2, CD005083

10.2165/11538930-000000000-00000

10.1016/j.burns.2013.06.014

10.1111/j.1524-475X.2010.00608.x

Thông tin
Thông tin xuất bản

Wound Repair and Regeneration

Tập 24 Số 2

394-401

Thông tin tác giả