The structure of the Met144Leu mutant of copper nitrite reductase from Alcaligenes xylosoxidans provides the first glimpse of a protein–protein complex with azurin II
Tóm tắt
Cu-containing nitrite reductases (NiRs) perform the reduction of nitrite to NO via an ordered mechanism in which the delivery of a proton and an electron to the catalytic type 2 Cu site is highly orchestrated. Electron transfer from a redox partner protein, azurin or pseudoazurin, to the type 1 Cu site is assumed to occur through the formation of a protein–protein complex. We report here a new crystal form in space group P212121 of the Met144Leu mutant of NiR from Alcaligenes xylosoxidans (AxNiR), revealing a head-to-head packing motif involving residues around the hydrophobic patch of domain 1. Superposition of the structure of azurin II with that of domain 1 of one of the Met144Leu molecules provides the first glimpse of an azurin II–NiR protein–protein complex. Mutations of two of the residues of AxNiR, Trp138His (Barrett et al. in Biochemistry 43:16311–16319, 2004) and Met87Leu, highlighted in the AxNiR–azurin complex, results in substantially decreased activity when azurin is used as the electron donor instead of methyl viologen, providing direct evidence for the importance of this region for complex formation.
Tài liệu tham khảo
Zumft WG, Harder W, Schleifer KH (1992) In: Balows HG, Trüper A, Dworkin M (eds) The prokaryotes: a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, vol III. Springer, New York, pp 554–582
Zumft WG (1997) Microbiol Mol Biol Rev 61:533–616
Eady RR, Hasnain SS (2003) In: McCleverty JA, Meyer TJ (eds) Comprehensive coordination chemistry II, vol 1. Elsevier, Amsterdam, pp 759–786
Hough MA, Ellis MJ, Antonyuk S, Strange RW, Sawers G, Eady RR, Hasnain SS (2005) J Mol Biol 350:300–309
Strange RW, Murphy LM, Dodd FE, Abraham ZH, Eady RR, Smith BE, Hasnain SS (1999) J Mol Biol 287:1001–1009
Murphy LM, Dodd FE, Yousafzai FK, Eady RR, Hasnain SS (2002) J Mol Biol 315:859–871
Boulanger MJ, Kukimoto M, Nishiyama M, Horinouchi S, Murphy MEP (2000) J Biol Chem 275:23957–23964
Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS (2005) Proc Natl Acad Sci USA 102:12041–12046
Tocheva EI, Rosell FI, Mauk AG, Murphy MEP (2004) Science 304:867–870
Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS (2004) Inorg Chem 43:7591–7593
Dodd FE, Van Beeumen J, Eady RR, Hasnain SS (1998) J Mol Biol 282:369–382
Ellis MJ, Dodd FE, Sawers G, Eady RR, Hasnain SS (2003) J Mol Biol 328:429–438
Kukimoto M, Nishiyama M, Ohnuki T, Turley S, Adman ET, Horinouchi S, Beppu T (1995) Protein Eng 8:153–158
Kukimoto M, Nishiyama M, Tanokura M, Adman ET, Horinouchi S (1996) J Biol Chem 271:13680–13683
Kukimoto M, Nishiyama M, Tanokura M, Murphy MEP, Adman ET, Horinouchi S (1996) FEBS Lett 394:87–90
Dodd FE, Hasnain SS, Hunter WN, Abraham ZHL, Debenham M, Kanzler H, Eldridge M, Eady RR, Ambler RP, Smith BE (1995) Biochemistry 34:10180–10186
Murphy MEP, Turley S, Adman ET (1998) In: Canters GW, Vijgenboom E (eds) Biological electron transfer chains: genetics, composition, and mode of operation. NATO science series. Kluwer, Dordrecht, pp 115–128
Tang C, Iwahara J, Clore GM (2006) Nature 444:383–386
Blundell TL, Recio JF (2006) Nature 444:279–280
Barrett ML, Harris RL, Antonyuk S, Hough MA, Ellis MJ, Sawers G, Eady RR, Hasnain SS (2004) Biochemistry 43:16311–16319
Dodd FE, Abraham ZHL, Eady RR, Hasnain SS (2000) Acta Crystallogr Sect D 56:690–696
Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW (1991) J Mol Biol 221:765–772
Hough MA, Hall JF, Kanbi LD, Hasnain SS (2001) Acta Crystallogr Sect D 57:355–360
Wijma HJ, Jeuken LJC, Verbeet MP, Armstrong FA, Canters GW (2006) J Biol Chem 281:16340–16346
Paraskevopoulos K, Sundararajan M, Surendran R, Hough MA, Eady RR, Hillier IH, Hasnain SS (2006) Dalton Trans 25:3067–3076
Otwinowski Z, Minor W (1997) In: Carter CWJ, Sweet RM (eds) Methods in enzymology: macromolecular crystallography, part A 276. Academic, New York, pp 307–326
Vagin A, Teplyakov A (1997) J Appl Crystallogr 30:1022–1025
Murshudov GN, Vagin AA, Dodson EJ (1997) Acta Crystallogr Sect D 53:240–255
CCP4 (1994) Acta Crystallogr Sect D 50:760–763
Kleywegt GJ, Jones TA (1996) Acta Crystallogr Sect D 52:829–832
Lamzin VS (1993) Acta Crystallogr Sect D 49:129–17
Laskowski RA, MacArthur MW, Moss DS, Thornton JM (1993) J Appl Crystallogr 25:283–291
Ramachandran GN, Ramakrishnan C, Sasisekharan V (1963) J Mol Biol 7:955
Cruickshank DWJ (1996) Proceedings of CCP4 study weekend: refinement of macromolecular structures, Chester
Nicholls A, Sharp KA, Honig B (1991) Proteins Struct Funct Genet 11:281–296
Groeneveld CM, Canters GW (1985) Eur J Biochem 153:559–564
Abraham ZHL, Lowe DJ, Smith BE (1993) Biochem J 295:587–593