Sự phân hóa tự phát và mất chromosome ở iPSCs của hội chứng trisomy 18 ở người
Tóm tắt
Aneuploidy, bao gồm cả trisomy, dẫn đến những khuyết tật phát triển và là nguyên nhân hàng đầu gây sẩy thai ở người. Khác với trisomy 21, các cơ chế bệnh lý của trisomy 18 vẫn chưa rõ ràng. Ở đây, chúng tôi đã thành công trong việc tạo ra các tế bào gốc trung mô cảm ứng (iPSCs) từ các tế bào dịch ối của người (AFCs) trong các thai kỳ có trisomy 18. Chúng tôi phát hiện rằng các iPSCs 18T (trisomy 18) có xu hướng phân hóa một cách tự phát. Điều thú vị, sau 10 thế hệ, các 18T-iPSCs đã mất đi 18 chromosome dư thừa và chuyển đổi thành các tế bào lưỡng bội. Phân tích hybridization huỳnh quang
Từ khóa
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