The roles of different salts and a novel osmotic pressure control strategy for improvement of DHA production by Schizochytrium sp.

Bioprocess and Biosystems Engineering - Tập 38 - Trang 2129-2136 - 2015
Xue-Chao Hu1, Lu-Jing Ren1, Sheng-Lan Chen1, Li Zhang1, Xiao-Jun Ji1, He Huang1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People’s Republic of China

Tóm tắt

The effects of different osmotic pressure, changed by six salts (NaCl, Na2SO4, (NH4)2SO4, KH2PO4 and MSG), on cell growth and DHA synthesis by Schizochytrium sp. were investigated. Six optimal mediums were obtained to study different osmotic pressure combinations at cell growth stage and DHA synthesis stage. Results showed that cultivated cell in higher osmotic pressure condition and fermented in lower osmotic pressure condition was benefit to enhance DHA synthesis. Combination 17-6 could get the maximum cell dry weight of 56.95 g/L and the highest DHA percentage in total fatty acids of 55.21 %, while combination 17-B could get the highest lipid yield of 33.47 g/L with 42.10 % DHA in total fatty acids. This was the first report about the enhancement of DHA production by osmotic regulation and this work provided two novel osmotic control processes for high lipid yield and high DHA percentage in total fatty acids.

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