The role of luminal factors in the recovery of gastric function and behavioral changes after chronicHelicobacter pyloriinfection

American Journal of Physiology - Gastrointestinal and Liver Physiology - Tập 295 Số 4 - Trang G664-G670 - 2008
Elena F. Verdú1, Přemysl Berčík, Xian Xi Huang, Jun Lü, Nafia Al-Mutawaly, Hiromi Sakai, Thomas A. Tompkins, Kenneth Croitoru, Eihun Tsuchida, Mary H. Perdue, Stephen M. Collins
1McMaster Univ. HSC 3N49C, 1200 Main St. West, Hamilton, Ontario, Canada. [email protected]

Tóm tắt

The role of chronic infections, such as Helicobacter pylori ( Hp), to produce sustained changes in host physiology remains controversial. In this study, we investigate whether the antigenic or bacterial content of the gut, after Hp eradication, influences the changes in gut function induced by chronic Hp infection. Mice were infected with Hp for 4 mo and then treated with antibiotics or placebo for 2 wk. Gastric emptying was measured using videofluoroscopy, feeding behavior using a 24-h feeding system, and intestinal permeability using an isolated jejunal segment arterially perfused with an artificial oxygen carrier, hemoglobin vesicles. Immune responses were assessed by CD3+cell counts and anti- Hp antibodies using ELISA. To determine the role of luminal factors in host physiology posteradication, groups of mice received the probiotics containing Lactobacillus rhamnosus R0011 and L. helveticus R0052 or placebo for 2 wk or crude Hp antigen weekly for 2 mo. Chronic Hp infection was associated with delayed gastric emptying, increased intestinal permeability, and increased gastric CD3+cell counts. Hp-induced altered feeding patterns did not reverse after eradication. Probiotics accelerated the recovery of paracellular permeability and delayed gastric emptying, improved the CD3+cell counts, and normalized altered feeding patterns posteradication. Hp antigen resulted in increased anti- Hp antibodies and increased CD3+cell counts in the stomach and delayed recovery of gastric function. Our results suggest that the bacterial content of the gut, as well as the presence of relevant antigens, influences the rate of recovery of host pathophysiology induced by chronic Hp infection. These changes do not seem to occur in association with modulation of intestinal permeability.

Từ khóa


Tài liệu tham khảo

10.1016/0016-5085(94)90698-X

10.1053/gast.2002.36024

Bercik P, Wang L, Kean I, Verdu EF, Tougas G, Collins SM.Persistence of sensory and eating disturbances afterH. pylorieradication in mice.Gastroenterology122: W1074, 2002. (Abstract)

10.1056/NEJM199812243392602

Di Leo V, D'Inca R, Bettini MB, Podswiadek M, Punzi L, Mastropaolo G, Sturniolo GC.Effect ofHelicobacter pyloriand eradication therapy on gastrointestinal permeability. Implications for patients with seronegative spondyloarthritis.J Rheumatol32: 295–300, 2005.

10.1128/IAI.73.12.7844-7852.2005

Graham DY, Klein PD.Campylobacter pyloridis gastritis: the past, the present and speculations about the future (Review).Am J Gastroenterol82: 283–286, 1987.

10.1016/S0140-6736(96)90341-4

Jiang B, Jordana M, Xing Z, Smaill F, Snider DP, Borojevic R, Steele-Norwood D, Hunt RH, Croitoru K.Replication-defective adenovirus infection reducesHelicobacter feliscolonization in the mouse in a gamma interferon- and interleukin-12-dependent manner.Infect Immun67: 4539–4544, 1999.

10.1023/B:DDAS.0000037794.02040.c2

Malfertheiner P, Fischbach W, Layer P, Moessner J, Stolte M, Leodolter A, Demleitner K, Fuchs WA.ELAN study proves symptomatic benefit ofHelicobacter pylorieradication in functional dyspepsia.Gastroenterology118: A440, 2000. (Abstract)

10.1053/j.gastro.2006.05.053

Marshall BJ, Warren JR.Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.Lancet16: 1311–1315, 1984.

10.1097/00042737-199912000-00006

Matysiak-Budnik T, Terpend K, Alain S, Sanson le Pors MJ, Desjeux JF, Mégraud F, Heyman M.Helicobacter pylorialters exogenous antigen absorption and processing in a digestive tract epithelial cell line model.Infect Immun66: 5785–5791, 1998.

10.1056/NEJM199812243392601

10.1053/j.gastro.2005.04.012

Sakai H, Sou K, Horinouchi H, Kobayashi K, Tsuchida E.Hemoglobin vesicles as artificial oxygen carriers: present situation and future visions.J Intern Med263: 4–15, 2008.

10.1136/gut.47.6.804

10.1053/gast.2002.33663

10.1136/bmj.318.7187.833

10.1056/NEJM199910073411502

10.1053/j.gastro.2006.02.007

10.1136/gut.2003.021154

10.1136/gut.2005.080739

Thông tin
Thông tin xuất bản

American Journal of Physiology - Gastrointestinal and Liver Physiology

Tập 295 Số 4

G664-G670

Thông tin tác giả