The role of calcium in the acrosome reaction: An analysis using ionophore A23187

Wiley - Tập 198 Số 3 - Trang 383-392 - 1976
Prudence Talbot1, Robert G. Summers2, Bonnie L. Hylander1, Ellen M. Keough1, L. E. Franklin1
1Department of Biology, University of Houston, Houston, Texas 77004.
2Department of Zoology, University of Maine, Orono, Maine 04473.

Tóm tắt

AbstractThe role of Ca+2 in the acrosome reaction of echinoid and mammalian sperm was investigated using the Ca+2 transporting ionophore A23187. The ionophore induced morphologically normal acrosome reactions in both types of sperm (as assessed by electron microscopic observation of echinoid sperm and phase contrast microscopic observation of mammalian sperm). In echinoids, these reactions were immediate. In the guinea pig and hamster, ionophore significantly decreased the capacitation interval; early reactions were accompanied by activation of motility. Ionophore induced reactions were affected by sperm, ionophore and Ca+2 concentrations. Since both ionophore induced and natural reactions require extracellular Ca+2, it is suggested that an influx of Ca+2 represents the initial step of the acrosome reaction. Under natural conditions, the permeability change which results in Ca+2 influx may be induced in echinoid sperm by egg jelly and may occur in mammalian sperm during capacitation.Ionophore A23187 should prove an experimentally useful drug for further study of the acrosome reaction since its effect on cells is understood, it induces synchronous reactions in a high percentage of sperm, and it conveniently reduces the capacitation interval in mammalian sperm.

Từ khóa


Tài liệu tham khảo

10.1530/jrf.0.0440155

10.1007/978-1-4684-2892-6_1

10.1530/jrf.0.0340547

Cavanaugh G. M., 1956, Formulae and methods V. of the Marine Biological Laboratory Chemical Room. Fifth ed, 87

10.1016/0006-291X(74)90181-8

10.1016/0012-1606(76)90182-2

10.2307/1538830

Colwin L. H., 1967, Fertilization. Comparative Morphology, Biochemistry and Immunology, 295

10.2307/1538405

10.2307/1538583

10.1016/S0074-7696(08)62576-0

Dan J. C., 1967, Fertilization. Comparative Morphology, Biochemistry and Immunology, 237

10.1016/0014-4827(72)90567-8

10.1095/biolreprod14.4.405

10.1016/0014-4827(74)90814-3

10.1016/S0021-9258(19)44681-4

10.1002/jez.1401930323

10.1083/jcb.66.1.86

Sokal R. R., 1969, Biometry: The Principles and Practice of Statistics in Biological Research

10.1073/pnas.71.5.1915

10.1038/252041a0

10.1007/BF00220142

10.1002/jez.1401960312

10.1530/jrf.0.0360429

10.1530/jrf.0.0230193

10.1016/0014-4827(74)90199-2

Thông tin
Thông tin xuất bản

Wiley

Tập 198 Số 3

383-392

Thông tin tác giả