The quantitative significance of <i>Syntrophaceae</i> and syntrophic partnerships in methanogenic degradation of crude oil alkanes

Wiley - Tập 13 Số 11 - Trang 2957-2975 - 2011
Neil Gray1, Angela Sherry1, Russell J. Grant1, Arlene K. Rowan1, Casey R. J. Hubert1, Cameron M. Callbeck2,3, Carolyn M. Aitken1, D. M. Jones1, Jennifer J. Adams4, Steve Larter3, Ian M. Head1
1School of Civil Engineering & Geosciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK
2Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, UK.
3School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK
4Departments of Geoscience

Tóm tắt

SummaryLibraries of 16S rRNA genes cloned from methanogenic oil degrading microcosms amended with North Sea crude oil and inoculated with estuarine sediment indicated that bacteria from the genera Smithella (Deltaproteobacteria, Syntrophaceace) and Marinobacter sp. (Gammaproteobacteria) were enriched during degradation. Growth yields and doubling times (36 days for both Smithella and Marinobacter) were determined using qPCR and quantitative data on alkanes, which were the predominant hydrocarbons degraded. The growth yield of the Smithella sp. [0.020 g(cell‐C)/g(alkane‐C)], assuming it utilized all alkanes removed was consistent with yields of bacteria that degrade hydrocarbons and other organic compounds in methanogenic consortia. Over 450 days of incubation predominance and exponential growth of Smithella was coincident with alkane removal and exponential accumulation of methane. This growth is consistent with Smithella's occurrence in near surface anoxic hydrocarbon degrading systems and their complete oxidation of crude oil alkanes to acetate and/or hydrogen in syntrophic partnership with methanogens in such systems. The calculated growth yield of the Marinobacter sp., assuming it grew on alkanes, was [0.0005 g(cell‐C)/g(alkane‐C)] suggesting that it played a minor role in alkane degradation. The dominant methanogens were hydrogenotrophs (Methanocalculus spp. from the Methanomicrobiales). Enrichment of hydrogen‐oxidizing methanogens relative to acetoclastic methanogens was consistent with syntrophic acetate oxidation measured in methanogenic crude oil degrading enrichment cultures. qPCR of the Methanomicrobiales indicated growth characteristics consistent with measured rates of methane production and growth in partnership with Smithella.

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Wiley

Tập 13 Số 11

2957-2975

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