The novel bZIP transcription factor Fpo1 negatively regulates perithecial development by modulating carbon metabolism in the ascomycete fungus Fusarium graminearum

Wiley - Tập 22 Số 7 - Trang 2596-2612 - 2020
Jiyoung Shin1, Duc‐Cuong Bui1, Sieun Kim1, So Yun Jung1, Hye Jin Nam1, Jae Yun Lim1, Gyung Ja Choi2, Yin‐Won Lee1, Jung‐Eun Kim3, Hokyoung Son1,3
1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea
2Therapeutic & Biotechnology Division Center for Eco‐friendly New Materials, Korea Research Institute of Chemical Technology Daejeon 34114 Republic of Korea
3Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

Tóm tắt

Summary

Fungal sexual reproduction requires complex cellular differentiation processes of hyphal cells. The plant pathogenic fungus Fusarium graminearum produces fruiting bodies called perithecia via sexual reproduction, and perithecia forcibly discharge ascospores into the air for disease initiation and propagation. Lipid metabolism and accumulation are closely related to perithecium formation, yet the molecular mechanisms that regulate these processes are largely unknown. Here, we report that a novel fungal specific bZIP transcription factor, F. graminearum perithecium overproducing 1 (Fpo1), plays a role as a global transcriptional repressor during perithecium production and maturation in F. graminearum. Deletion of FPO1 resulted in reduced vegetative growth, asexual sporulation and virulence and overproduced perithecium, which reached maturity earlier, compared with the wild type. Intriguingly, the hyphae of the fpo1 mutant accumulated excess lipids during perithecium production. Using a combination of molecular biological, transcriptomic and biochemical approaches, we demonstrate that repression of FPO1 after sexual induction leads to reprogramming of carbon metabolism, particularly fatty acid production, which affects sexual reproduction of this fungus. This is the first report of a perithecium‐overproducing F. graminearum mutant, and the findings provide comprehensive insight into the role of modulation of carbon metabolism in the sexual reproduction of fungi.

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Wiley

Tập 22 Số 7

2596-2612

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