The new ID‐heparin/PF4 antibody test for rapid detection of heparin‐induced antibodies in comparison with functional and antigenic assays

British Journal of Haematology - Tập 116 Số 4 - Trang 887-891 - 2002
Petra Eichler1, Ricarda Raschke2, Norbert Lübenow2, Oliver Meyer3, Peter Schwind4, Andreas Greinacher2
1Institute for Immunology and Transfusion Medicine, Ernst-Moritz-Arndt-University, Greifswald, Germany
2Institute for Immunology and Transfusion Medicine, Ernst‐Moritz‐Arndt‐University, Greifswald,
3Blood Bank, Charite Campus Virchow-Clinic, Humboldt-University, Berlin, Germany, and
4DiaMed SA, Cressier sur Morat, Switzerland

Tóm tắt

Summary. Heparin‐induced thrombocytopenia (HIT) is an immune‐mediated complication of heparin treatment. Several in vitro assays are available to detect the causative HIT antibodies: functional assays, usually requiring freshly prepared platelets and immunological tests based on the enzyme‐linked immunosorbent assay (ELISA) principle. We compared a new, simple and rapid test based on the ID‐microtyping particle agglutination system with 14C‐serotonin release assay, heparin‐induced platelet activation (HIPA) test and two ELISAs. Sera from 100 confirmed HIT patients, 20 serologically negative suspected HIT patients and 20 healthy blood donors were used. The specificity and sensitivity of the new test was similar to the functional assays. Compared with the ELISAs, specificity was better at the cost of reduced sensitivity. As in all other immunological tests, HIT antibodies against less typical antigens, such as interleukin (IL)‐8 or neutrophil‐activating peptide (NAP) 2 could not be detected. Thus, although the ID‐Heparin/PF4 antibody test seems to be a quick, reliable and robust test to determine the presence of HIT antibodies, it should still be combined with a functional assay if possible. Evaluation of the test in a prospective setting as well as interlaboratory variation should be assessed as a next step.

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Thông tin xuất bản

British Journal of Haematology

Tập 116 Số 4

887-891

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