The networks of human gut microbe–metabolite associations are different between health and irritable bowel syndrome

ISME Journal - Tập 9 Số 8 - Trang 1899-1903 - 2015
Vijay Shankar1, Daniel Homer1, Laura Rigsbee1, Harry J. Khamis2, Sonia Michail3, Michael L. Raymer4, Nicholas V. Reo1, Oleg Paliy1
1Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH, USA
2Department of Mathematics and Statistics, Wright State University, Dayton, OH, USA
3Department of Pediatrics, Wright State University , Dayton, OH, USA
4Department of Computer Sciences; Wright State University; Dayton, OH USA

Tóm tắt

Abstract The goal of this study was to determine if fecal metabolite and microbiota profiles can serve as biomarkers of human intestinal diseases, and to uncover possible gut microbe–metabolite associations. We employed proton nuclear magnetic resonance to measure fecal metabolites of healthy children and those diagnosed with diarrhea-predominant irritable bowel syndrome (IBS-D). Metabolite levels were associated with fecal microbial abundances. Using several ordination techniques, healthy and irritable bowel syndrome (IBS) samples could be distinguished based on the metabolite profiles of fecal samples, and such partitioning was congruent with the microbiota-based sample separation. Measurements of individual metabolites indicated that the intestinal environment in IBS-D was characterized by increased proteolysis, incomplete anaerobic fermentation and possible change in methane production. By correlating metabolite levels with abundances of microbial genera, a number of statistically significant metabolite–genus associations were detected in stools of healthy children. No such associations were evident for IBS children. This finding complemented the previously observed reduction in the number of microbe–microbe associations in the distal gut of the same cohort of IBS-D children.

Từ khóa


Tài liệu tham khảo

Bergey, 1994, Bergey’s Manual of Determinative Bacteriology

Bernalier-Donadille, 2010, Fermentative metabolism by the human gut microbiota, Gastroenterol Clin Biol, 34, S16, 10.1016/S0399-8320(10)70016-6

Chatterjee, 2007, The degree of breath methane production in IBS correlates with the severity of constipation, Am J Gastroenterol, 102, 837, 10.1111/j.1572-0241.2007.01072.x

David, 2014, Diet rapidly and reproducibly alters the human gut microbiome, Nature, 505, 559, 10.1038/nature12820

Duboc, 2012, Increase in fecal primary bile acids and dysbiosis in patients with diarrhea-predominant irritable bowel syndrome, Neurogastroenterol Motil, 24, 513, 10.1111/j.1365-2982.2012.01893.x

Duncan, 2002, Acetate utilization and butyryl coenzyme A (CoA):acetate-CoA transferase in butyrate-producing bacteria from the human large intestine, Appl Environ Microbiol, 68, 5186, 10.1128/AEM.68.10.5186-5190.2002

Ghoshal, 2011, Slow transit constipation associated with excess methane production and its improvement following rifaximin therapy: a case report, J Neurogastroenterol Motil, 17, 185, 10.5056/jnm.2011.17.2.185

Gorkiewicz, 2013, Alterations in the colonic microbiota in response to osmotic diarrhea, PLoS One, 8, e55817, 10.1371/journal.pone.0055817

Guilloteau, 2010, From the gut to the peripheral tissues: the multiple effects of butyrate, Nutr Res Rev, 23, 366, 10.1017/S0954422410000247

Holdeman, 1974, New genus, Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human feces, Int J Syst Evol Microbiol, 24, 260

Iakiviak, 2011, Functional analyses of multiple lichenin-degrading enzymes from the rumen bacterium Ruminococcus albus 8, Appl Environ Microbiol, 77, 7541, 10.1128/AEM.06088-11

Karasov, 2009, Metabolic teamwork between gut microbes and hosts, Microbe, 4, 323

Martens, 2011, Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts, PLoS Biol, 9, e1001221, 10.1371/journal.pbio.1001221

Quigley, 2012, A global perspective on irritable bowel syndrome: a consensus statement of the World Gastroenterology Organisation Summit Task Force on irritable bowel syndrome, J Clin Gastroenterol, 46, 356, 10.1097/MCG.0b013e318247157c

Rigsbee, 2012, Quantitative profiling of gut microbiota of children with diarrhea-predominant irritable bowel syndrome, Am J Gastroenterol, 107, 1740, 10.1038/ajg.2012.287

Rohlf, 1990, Extensions of the procrustes method for the optimal superimposition of landmarks, Syst Zool, 39, 40, 10.2307/2992207

Sekirov, 2010, Gut microbiota in health and disease, Physiol Rev, 90, 859, 10.1152/physrev.00045.2009

Shankar, 2013, Do gut microbial communities differ in pediatric IBS and health?, Gut Microbes, 4, 347, 10.4161/gmic.24827

Steck, 2011, Bacterial proteases in IBD and IBS, Gut, 61, 1610, 10.1136/gutjnl-2011-300775

Takahashi, 2000, Pathways for amino acid metabolism by Prevotella intermedia and Prevotella nigrescens, Oral Microbiol Immunol, 15, 96, 10.1034/j.1399-302x.2000.150205.x

Watanabe, 2012, Characterization of Phascolarctobacterium succinatutens sp. nov., an asaccharolytic, succinate-utilizing bacterium isolated from human feces, Appl Environ Microbiol, 78, 511, 10.1128/AEM.06035-11

Thông tin
Thông tin xuất bản

ISME Journal

Tập 9 Số 8

1899-1903

Thông tin tác giả